Experiments on collection and preparation of biological materials for laboratory animals
The study of the toxic effects of foreign compounds. It is often necessary to determine the concentrations of compounds or their metabolites in the blood, urine
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Improvement of the Koch method of acute toxicity testing
1, study the design of acute toxicity test of chemicals, master the determination of LD50. 2. Observe the toxic reaction of phoxim.
Laboratory animal grouping, labeling, and dyeing experiments
The grouping, labeling and reasonable dyeing of experimental animals are the prerequisites for obtaining good experimental results and conclusions, and are also
Extracellular matrix (ECM) preparation experiments
Experiments in the preparation of extracellular matrix can be used to (1) study the constituents of the extracellular matrix as well as its physiological charac
Operating on an open workbench
Clean and wipe the work area, place the reagent bottles, pipette, etc., on the bench (Figure 6.5), and make preparations. If necessary. If necessary, flame ste
Experimental purification of λ phage particles by precipitation/centrifugation
If the purpose of purification of the phage pellet is to obtain outgrown DNA fragments for subsequent subcloning or to prepare phage two-arms, centrifugation ca
Electrical activity of single neurons: the membrane clamp technique
Over the last 20 years, the membrane clamp technique has become one of the main research tools in modern electrophysiology. Initially used only to measure singl
High-throughput holistic in situ hybridization for the detection of environmental disturbances in zebrafish (Danio rerio), an embryonic tissue-specific gene expression alteration experiment
Holistic in situ hybridization is a process that allows observation of cellular gene expression (mRNA) in intact organisms. By comparing differences in gene exp
Restriction enzyme digestion labeling genome scanning for mutation detection experiments
Today, there is a need for more effective methods in the detection of mutations in humans and other organisms caused by environmental toxins and radiation expos