3,200+ step-by-step lab protocols and SOPs for cell culture, staining, PCR, protein work and microbiology, with reagents, conditions and evaluation criteria.
This new generation of sperm F I S H assays has identified multiple paternal risk factors such as age, polypharmacy, lifestyle, and environmental aspects and oc
In physics, any electric current is accompanied by the production of a magnetic field. Ion currents flowing inside the brain and nerves also generate magnetic f
This chapter deals with two main aspects: (i) the methodology for simultaneous recording of EMG and video on normally moving animals, and (ii) the timing and ne
The purpose of this chapter is first to introduce the technique to scientists who wish to use it in a practical way. Secondly, typical examples of the results o
Nuclear transplantation is based on the theory that all nuclei from the same embryo or cell line contain exactly the same genetic information as the fertilized
Positron emission tomography (PET) is an in vivo functional neuroimaging technique that provides three-dimensional quantitative images of a wide range of physio
The techniques discussed in this chapter allow recording under local anesthesia of the activity of cortical and subcortical structures such as the thalamus, bas
This chapter is intended as a practical guide for researchers who need to modify antibodies themselves. It covers common chemical and protein hydrolysis modific
This experimental protocol describes several methods for generating complement-stabilized lentiviral vectors either by using fusion packaging proteins containin
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