4L Batch Stirred Suspension Culture
Cells were cultured on a small scale and then added to a culture aspiration flask pre-moderated and pre-filled with 5% CO2. The cell suspension was slowly agita
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Experiments on Tec eukaryotic expression vector construction and its effect on cell signaling
Tec (Tyrosinekinaseexpressedinhepatocelluar carcinoma) is an important non-receptor tyrosine kinase, first cloned by Mano et al, from liver cancer tissue. The h
Preparation and Sterilization of Ultra Pure Water (UPW)
This lab was derived from: Animal Cell Culture - A Guide to Basic Techniques (5th Edition)
125I-UdR release assay to determine NK cell activity
Natural killer (NK) cells are involved in the first line of the body's immune defense and are mainly used in (1) anti-tumor and anti-virus (2) immunomodulation
Preparation of medium with 1× stock solution
Check the design of the formulation: if the formulation is complete, it can be used directly after the addition of serum; if the formulation is incomplete (e.g.
Lymphocyte proliferation test
Lymphocyte proliferation test, usually under the stimulation of antigenic substances such as mitogens and other antigenic substances, proliferation, differentia
DAPI staining
Cell staining with DAPI
NK cell activity assay: LDH method
This experiment was mainly used to determine NK cell activity.
Mixed lymphocyte proliferation assay
The mixed lymphocyte proliferation assay, also known as mixed lymphocyte culture (MLC) or as mixed lymphocyte response (MLR), is commonly used in tissue matchin