Detection of apoptosis in tumor cells by DNA degradation and fracture assay
DNA degradation fracture assay for detection of apoptosis in tumor cells can be used to (1) detect apoptosis in tumor cells and (2) detect the effect of drug tr
Knowledge Base
Laboratory Protocols & SOPs Library
3,200+ step-by-step lab protocols and SOPs for cell culture, staining, PCR, protein work and microbiology, with reagents, conditions and evaluation criteria.
3228 articles
Experiments on Tec eukaryotic expression vector construction and its effect on cell signaling
Tec (Tyrosinekinaseexpressedinhepatocelluar carcinoma) is an important non-receptor tyrosine kinase, first cloned by Mano et al, from liver cancer tissue. The h
Detection of Caspase-3 activity in apoptotic cells
The detection of Caspase-3 activity in apoptosis is mainly applied to (1) enzyme viability assay (2) apoptosis index assay.
Sample preparation experiments for DNA fluorescence staining
This experimental method was obtained from the official website of the Fourth Military Medical University
Preparation and analysis of cellular RNA content samples
This experiment is based on "Color Atlas of Practical Flow Cytometry", edited by Shukui Wang and Zhenying Zhou.
Calcein release assay to detect cytotoxic T-lymphocyte activity assay
The source of this experiment is the official website of the Fourth Military Medical University
NK cell activity assay by lactate dehydrogenase (LDH) release assay
The source of this experiment is the official website of the Fourth Military Medical University
Apoptosis induced by staurosporine
Staurosporine is a potent inhibitor of protein kinase C and most other kinases, including tyrosine protein kinases. Directly inhibits topoisomerase II activity
TGF-f3/Smad signaling pathway-related protein expression
Transforming growth factor (TGF)-13/Smads signaling pathway related protein expression is mainly used to: (1) study the expression of related proteins in the TG