3,200+ step-by-step lab protocols and SOPs for cell culture, staining, PCR, protein work and microbiology, with reagents, conditions and evaluation criteria.
Non-viral vector plasmid DNA can be delivered to the human brain via the vascular route across the blood-brain barrier by intravenous injection of DNA encapsula
A technique for preparing transgenic mice by prokaryotic microinjection is described. Prokaryotic microinjection, which involves the injection of DNA into haplo
Proteins are diverse in nature, which makes it challenging to express and purify proteins using parallel methods. Parallel methods can especially be applied whe
Membrane proteins provide the appropriate molecular mechanisms that allow the controlled entry of useful molecules into the cell and permit the export of intrac
Describes the methods used to isolate the oxygen-sensitive iron-sulfur protein FNR in the laboratory.
Authors: Burgess et al., Translator: Chen Wei, this experi
The S D S -- P A G E methods presented in this book will fulfill the needs of most applications in this field.
Authors: Burgess et al, Translator: Chen Wei, Thi
Summarizes the application areas of IMAC, including protein hierarchical separation and proteomics, protein fixation and detection, as well as some special appl
The size or apparent atomic mass of proteins is perhaps the most common way of distinguishing the nature of a molecule. As a basis for many separation methods,
Hydrophobic interaction chromatography (HIC) is a valuable tool in protein purification technology. HIC is used for protein purification on a wide range of scal
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