3,200+ step-by-step lab protocols and SOPs for cell culture, staining, PCR, protein work and microbiology, with reagents, conditions and evaluation criteria.
A large number of non-viral systems have been used for nucleic acid delivery to cultured cells in vitro or to specific cells in vivo. They vary considerably in
Polylysine and its copolymers have gained wide application as non-viral gene vectors. Although polylysine itself is toxic to cells, the covalent attachment of p
It mainly describes the methods used to recover infectious influenza viruses from cDNA clones. These techniques are widely used in the integration and expressio
In proteomics research, a key step in all enrichment techniques is the subcellular fractionation, which is particularly important for the analysis of intracellu
A technique for preparing transgenic mice by prokaryotic microinjection is described. Prokaryotic microinjection, which involves the injection of DNA into haplo
This chapter describes the use of lentiviral vectors to transfer genes and small interfering RNA (siR N A ) expression cassettes to preimplantation crystalline
Efficient delivery of ΦC31 integrase and donor plasmids to recipient tissues or cells of interest remains the most challenging element of this integrative syst
Non-viral vector plasmid DNA can be delivered to the human brain via the vascular route across the blood-brain barrier by intravenous injection of DNA encapsula
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