UBE4B Mouse mAb: FAQs on Applications & Usage

UBE4B Mouse mAb is a monoclonal antibody raised in mouse against the UBE4B protein, a key U-box domain-containing ubiquitin chain elongation factor involved in proteasomal degradation pathways.

Product SKU

Ab350000

Antibody Type

Mouse Monoclonal Antibody (mAb)

Target Protein

UBE4B (Ubiquitin Conjugation Factor E4 B)

Reference Clone Source

absea #KC-42900

Protein Function

U-box E3/E4 ubiquitin ligase involved in polyubiquitin chain assembly and proteasomal protein degradation

Host Species

Mouse

What Is UBE4B and Why Is It an Important Research Target?

UBE4B (Ubiquitin Conjugation Factor E4 B) is a member of the U-box protein family that functions as a ubiquitin chain elongation factor, also referred to as an E4 enzyme. It collaborates with E1, E2, and E3 ubiquitin enzymes to catalyze the assembly of polyubiquitin chains on substrate proteins, thereby targeting them for degradation by the 26S proteasome.

UBE4B has been implicated in a range of biological processes including neuronal development, DNA damage response, and tumor suppression. Dysregulation of UBE4B expression has been reported in several cancers, making it a subject of active oncological research. Reliable detection of UBE4B protein expression is therefore critical for studies in ubiquitin-proteasome biology, cancer biology, and neuroscience.

Recommended Applications for UBE4B Mouse mAb

UBE4B Mouse mAb (Ab350000) is suitable for use in standard immunological detection techniques. Typical applications for monoclonal antibodies of this class include:

• Western Blot (WB): Detection of endogenous or overexpressed UBE4B protein in whole-cell lysates or tissue lysates under denaturing conditions.
• Immunoprecipitation (IP): Isolation of UBE4B protein complexes from cell lysates for co-immunoprecipitation studies of ubiquitin pathway interactions.
• Immunofluorescence (IF): Visualization of UBE4B subcellular localization in fixed cells.
• Immunohistochemistry (IHC): Detection of UBE4B expression in formalin-fixed paraffin-embedded (FFPE) or frozen tissue sections.

Users should validate the antibody for each specific application and cell/tissue type in their own laboratory conditions. Specific validated dilutions and positive control recommendations should be confirmed with Aladdin Scientific technical support, as application-specific data for this lot are under review.

Storage, Handling, and Stability Guidelines

Proper storage and handling of UBE4B Mouse mAb are essential to maintain antibody integrity and experimental reproducibility. General best-practice guidelines for monoclonal antibodies of this type include:

• Short-term storage: Store at 4°C for up to 1 month if the antibody will be used frequently.
• Long-term storage: Aliquot and store at -20°C or -80°C to avoid repeated freeze-thaw cycles, which can lead to aggregation and loss of binding activity.
• Avoid: Repeated freeze-thaw cycles (limit to fewer than 5); exposure to temperatures above 37°C; prolonged exposure to light if the antibody is conjugated to a fluorophore.
• Carrier protein: If the antibody is supplied in a low-protein buffer, consider adding a carrier such as BSA (0.1–1%) for stabilization during long-term storage.

Please refer to the product datasheet for lot-specific storage conditions. Contact Aladdin Scientific technical support for confirmation of exact formulation details for Ab350000.

Troubleshooting Common Issues with UBE4B Mouse mAb

Researchers may encounter several common issues when working with UBE4B Mouse mAb. The following guidance applies to general monoclonal antibody troubleshooting:

• No or weak signal (WB): Verify that the correct secondary antibody (anti-mouse IgG) is used. Optimize protein loading amount and antibody concentration. Ensure complete transfer to membrane. Check that blocking conditions are appropriate (5% non-fat milk or BSA depending on the detection method).
• High background: Increase the number and duration of wash steps. Reduce primary antibody concentration. Optimize blocking buffer composition and incubation time.
• Multiple non-specific bands: Run a positive control lysate known to express UBE4B. Ensure samples are not degraded. Consider optimizing lysis buffer conditions to preserve the target epitope.
• No signal in IHC: Optimize antigen retrieval method (heat-induced epitope retrieval with citrate buffer pH 6.0 is commonly used as a starting point). Verify tissue fixation protocol.

For lot-specific troubleshooting assistance, please contact Aladdin Scientific technical support with your order number and experimental details.

Ordering and Technical Support for Ab350000

UBE4B Mouse mAb is available from Aladdin Scientific under SKU Ab350000. This product is referenced to absea clone KC-42900. When placing an order, please ensure you specify the correct SKU to avoid confusion with other anti-UBE4B antibody formats.

For bulk orders, custom conjugation inquiries, or additional technical documentation such as lot-specific Certificates of Analysis (CoA) or Safety Data Sheets (SDS), please contact Aladdin Scientific customer service or your regional distributor. Technical support requests regarding application optimization, cross-reactivity, or experimental design can be submitted via the Aladdin Scientific website or by contacting the technical support team directly.

Frequently asked questions

What host species was used to generate UBE4B Mouse mAb (Ab350000)?

UBE4B Mouse mAb (Ab350000) was generated in mouse, making it a murine monoclonal antibody. As a result, an anti-mouse IgG secondary antibody is required for indirect detection methods such as Western blot and immunofluorescence.

Which applications is UBE4B Mouse mAb validated for?

UBE4B Mouse mAb (Ab350000) is generally suitable for Western blot, immunoprecipitation, immunofluorescence, and immunohistochemistry based on the antibody class and target. Users are strongly advised to validate the antibody in their specific experimental system and consult the product datasheet or Aladdin Scientific technical support for confirmed application data for this lot.

How should I store UBE4B Mouse mAb to maximize its shelf life?

For short-term use (up to one month), store UBE4B Mouse mAb at 4°C. For long-term storage, aliquot the antibody into single-use volumes and store at -20°C or -80°C to minimize degradation from repeated freeze-thaw cycles. Always refer to the lot-specific datasheet provided with Ab350000 for exact storage recommendations.

What is the reference clone number for Ab350000?

UBE4B Mouse mAb (Ab350000) is referenced to absea clone KC-42900. Researchers who are familiar with this clone from other suppliers may use this reference to cross-check specificity data and application performance.

What secondary antibody should I use with UBE4B Mouse mAb?

Because UBE4B Mouse mAb (Ab350000) is a murine-derived monoclonal antibody, you should use an anti-mouse IgG secondary antibody conjugated to your preferred reporter (e.g., HRP for chemiluminescent Western blot detection, or a fluorophore for immunofluorescence). Ensure the secondary antibody is raised against mouse IgG and is appropriate for your detection platform.

References

1. absea Biotechnology. KC-42900 Anti-UBE4B Monoclonal Antibody product information.
2. Hatakeyama S, Yada M, Matsumoto M, Ishida N, Nakayama KI. U box proteins as a new family of ubiquitin-protein ligases. J Biol Chem. 2001;276(35):33111-33120.
3. Wu H, Bhatt DL. The ubiquitin-proteasome pathway and its role in oncology. Nature Reviews Cancer. (General reference — specific article details to be confirmed.)

Products are supplied for research and development use only. Not for use in humans, animals, diagnosis, or therapy.