GRADE & PURITYCarrier Free?Carrier-free — supplied without added carrier protein/stabilizer. Use when carriers (e.g. BSA) would interfere with conjugation or sensitive assays.Validated?Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated.ExactAb™?ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications.Azide Free?Azide-free — without sodium azide preservative. Use in conjugations, cell work, or assays where azide is toxic or inhibitory.High Performance?High-performance grade with optimized purity and performance characteristics. Use for sensitive analyses where ordinary grades fall short.See COA
Carrier Free, ExactAb™, Azide Free, Validated, High Performance, See COA Azide Free,Carrier Free,ExactAb™,High Performance,Validated for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
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Storage & shipping
Store at -20°C,Avoid repeated freezing and thawing Ships Ice chest + Ice pads Check lot-specific COA for exact specifications.
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Quality documents
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
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Literature proof
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Panoramica
Rabbit anti Human Prohibitin Antibody, Polyclonal, could be used for WB, IHC and so on. Application:
WB: 0.01-2 µg/mL IHC: 5-30 µg/mL
IF/ICC: 2.5 µg/mL
Protein Function: Prohibitin inhibits DNA synthesis. It has a role in regulating proliferation. As yet it is unclear if the protein or the mRNA exhibits this effect. May play a role in regulating mitochondrial respiration activity and in aging.
Specifications
Product Name
Prohibitin Antibody - Anticorpo primario, specific to Prohibitin, Rabbit IgG
Sinonimi
Epididymis luminal protein 215 antibody | Epididymis secretory sperm binding protein Li 54e antibody | HEL 215 antibody | HEL S 54e antibody | PHB antibody | PHB_HUMAN antibody | PHB1 antibody | Prohibitin antibody | PHB | PHB1 | Prohibitin | HEL-215 | HE
Specifiche e purezza
Carrier Free, ExactAb™, Azide Free, Validated, High Performance, See COA
Specie ospite
Coniglio
Specificità
Prohibitin
Immunogeno
Recombinant PHB expressed in E.coli (AA 177-272).
Controllo positivo
WB: HeLa, MCF7, Ramos, A431, HEK293, Hep G2, Jurkat, A549, NIH/3T3 and C6 cell lysates. IHC: Human breast cancer, rectum, thyroid cancer, liver, liver cancer tissues; Mouse cardiac muscle tissue. IF/ICC: HeLa cells.
Coniugazione
Unconjugated
Grado
Azide Free, Carrier Free, ExactAb™, High Performance, Validated
Isotipo
Rabbit IgG
SDS-PAGE
150 kDa
Metodo di purificazione
Antigen affinity purified + Protein A purified
Concentrazione
See COA
Stoccaggio e spedizione
Forma
Liquid
Condizioni di conservazione di stoccaggio
Store at -20°C,Avoid repeated freezing and thawing
Spedito in
Ice chest + Ice pads
Stabilità e conservazione
Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.
Immagini
Prohibitin Antibody (Ab123227) - Western Blot All lanes: Prohibitin Antibody (Ab123227) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 30 kDa Observed band size: 28 kDa Exposure time: 14.8 s
Prohibitin Antibody (Ab123227) - IHC DAB staining on IHC Samples: Human Breast cancer Tissue Primary Ab: 10 µg/mL Prohibitin Antibody (Ab123227) Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
Prohibitin Antibody (Ab123227) - IHC DAB staining on IHC Samples: Human Rectum Tissue Primary Ab: 10 µg/mL Prohibitin Antibody (Ab123227) Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
Prohibitin Antibody (Ab123227) - IHC DAB staining on IHC Samples: Human Thyroid cancer Tissue Primary Ab: 10 µg/mL Prohibitin Antibody (Ab123227) Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
Prohibitin Antibody (Ab123227) - IHC DAB staining on IHC Samples: Human Liver Tissue Primary Ab: 10 µg/mL Prohibitin Antibody (Ab123227) Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
Prohibitin Antibody (Ab123227) - IHC DAB staining on IHC Samples: Human Liver cancer Tissue Primary Ab: 10 µg/mL Prohibitin Antibody (Ab123227) Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
Prohibitin Antibody (Ab123227) - IHC DAB staining on IHC Sample: Mouse Cardiac Muscle Tissue Primary Ab: 30 µg/mL Prohibitin Antibody (Ab123227) Control: Used PBS instead of primary antibody Second Ab: 2 μg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
Prohibitin Antibody (Ab123227) - IF/ICC IF analysis of Prohibitin Antibody (Ab123227) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 5% BSA for 1 hour at room temperature. Cells were stained with Prohibitin Antibody (Ab123227) at 1/200 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Rabbit IgG H&L (AF647) (Ab181958) at a dilution of 1/1000 for 1 hour at room temperature in the dark (red). Cells were counterstained with DAPI (blue).
Applicazione
Applicazione
Dilution info
IHC
5-30 µg/mL
WB
0.01-2 µg/mL
IF/ICC
1/200
Documentazione
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
Determine the necessary mass, volume, or concentration for preparing a solution.
Dilution Calculator
Determine the dilution needed to prepare a stock solution.
Reconstitution Calculator
Recensioni
Recensioni dei clienti
Domande frequenti
What does ExactAb? mean?
ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.
What does Carrier Free mean?
Carrier Free indicates that the material has been processed and tested for specialty industrial processing. Specifications focus on the impurities that affect performance in that application — for example, metal content for electronics, particle size for batteries, refractive index for optics, or moisture content for moisture-sensitive processes.
How should this product be stored?
Store at ?20 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.
How is this product shipped?
This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.
What documentation is provided?
Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.
Which species reactivity has this antibody been validated against?
Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (Prohibitin Antibody); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.
Need help choosing the grade?
Our grade selection guide covers purity, stabilizer status, and application suitability for all variants in our catalog.
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Products are supplied to verified businesses, institutions, and qualified professionals for research and development use only. Not for use in humans, animals, diagnosis, or therapy.
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