GRADE & PURITYRecombinant?Recombinant — produced via recombinant expression for defined sequence and consistency. Use for reproducible, animal-free proteins of known origin.ExactAb™?ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications.Validated?Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated.0.7 mg/mL
ExactAb™, Validated, Recombinant, 0.7 mg/mL ExactAb™,Recombinant,Validated for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
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Storage & shipping
Store at -20°C,Avoid repeated freezing and thawing Ships Ice chest + Ice pads Check lot-specific COA for exact specifications.
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Quality documents
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
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Literature proof
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Panoramica
Rabbit anti Human MBD2 Antibody, Recombinant, could be used for WB, IHC, IF, ICC and so on. Application: WB: 1/500-1/1000 IHC: 1/50-1/100 IF/ICC: 1/50-1/200 Protein Function Binds CpG islands in promoters where the DNA is methylated at position 5 of cytosine within CpG dinucleotides. Binds hemimethylated DNA as well. Recruits histone deacetylases and DNA methyltransferases. Acts as transcriptional repressor and plays a role in gene silencing. Functions as a scaffold protein, targeting GATAD2A and GATAD2B to chromatin to promote repression. May enhance the activation of some unmethylated cAMP-responsive promoters.
Specifications
Product Name
Recombinant MBD2 Antibody - Anticorpo primario, specific to MBD2, Rabbit IgG
A synthetic peptide derived from human MBD2 (AA 216-234)
Controllo positivo
WB: A375, MCF7, HeLa, Raji, NIH/3T3 and C6 cell lysates. IHC: Human tonsil tissue. IF/ICC: HL-60 cells
Coniugazione
Unconjugated
Grado
ExactAb™, Recombinant, Validated
Isotipo
Rabbit IgG
SDS-PAGE
150 kDa
Metodo di purificazione
Antigen affinity purified
Concentrazione
0.7 mg/mL
Stoccaggio e spedizione
Forma
Liquid
Condizioni di conservazione di stoccaggio
Store at -20°C,Avoid repeated freezing and thawing
Spedito in
Ice chest + Ice pads
Stabilità e conservazione
Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.
Immagini
Recombinant MBD2 Antibody (Ab114412) - Western Blot All lanes: Recombinant MBD2 Antibody (Ab114412) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 43 kDa Observed band size: 30, 47 kDa. Recombinant MBD2 Antibody (Ab114412) recognizes two isoforms of MBD2. The observed MW is consistent with what has been described in the literature (PMID: 17353267). Exposure time: 20.0 s
Recombinant MBD2 Antibody (Ab114412) - IF/ICC Immunocytochemistry analysis of MBD2 (green) in HL-60 using Recombinant MBD2 Antibody (Ab114412), and DAPI(blue).
Recombinant MBD2 Antibody (Ab114412) - IHC Immunohistochemistry analysis of paraffin-embedded Human tonsil using Recombinant MBD2 Antibody (Ab114412). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
Applicazione
Applicazione
Dilution info
WB
1/500-1/1000
IF/ICC
1/50-1/200
IHC
1/50 - 1/100
Documentazione
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
Determine the necessary mass, volume, or concentration for preparing a solution.
Dilution Calculator
Determine the dilution needed to prepare a stock solution.
Reconstitution Calculator
Recensioni
Recensioni dei clienti
Domande frequenti
What does ExactAb? mean?
ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.
What does Recombinant mean?
Recombinant is a defined analytical purity tier suitable for workflows requiring a specific validated property. It specifies a minimum assay percentage, controlled limits on common impurities (water, heavy metals, residue on ignition), and lot-to-lot consistency. Recombinant is one of several purity tiers — choose based on the sensitivity and regulatory requirements of your method.
How should this product be stored?
Store at ?20 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.
How is this product shipped?
This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.
What documentation is provided?
Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.
Which species reactivity has this antibody been validated against?
Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (Recombinant MBD2 Antibody); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.
Need help choosing the grade?
Our grade selection guide covers purity, stabilizer status, and application suitability for all variants in our catalog.
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Products are supplied to verified businesses, institutions, and qualified professionals for research and development use only. Not for use in humans, animals, diagnosis, or therapy.
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