TOMM40 Antibody - Anticorpo primario, specific to TOMM40, Rabbit IgG

Cat. No.: Ab131963

GRADE & PURITY Carrier Free, ExactAb™, Validated, 0.5 mg/mL

Application

Flow Cytometry, IF/ICC, WB

Host species

Coniglio

Reactive species

Umano

Isotype

Rabbit IgG

Conjugation

Unconjugated

Panoramica

Rabbit anti Human TOMM40 Antibody, Polyclonal, could be used for WB, ICC, IF, Flow and so on.
Application:
WB: 1/2000
Flow: 1/25
ICC/IF: 1/25
Protein Function
Channel-forming protein essential for import of protein precursors into mitochondria.

Specifications

Product Name

TOMM40 Antibody - Anticorpo primario, specific to TOMM40, Rabbit IgG

Sinonimi

p38.5 | Protein Haymaker | Translocase of outer membrane 40 kDa subunit homolog | C19orf1 antibody | D19S1177E antibody | Haymaker protein antibody | Mitochondrial import receptor subunit TOM40 homolog antibody | Mitochondrial outer membrane protein antib

Specifiche e purezza

ExactAb™, Validated, Carrier Free, 0.5 mg/mL

Specie ospite

Coniglio

Specificità

TOMM40

Immunogeno

A KLH conjugated synthetic peptide from human TOMM40 (AA 22-56)

Controllo positivo

WB: HEK293, HeLa, A431, Hep G2 and SK-OV-3 cell lysates. ICC/IF: U2OS cells. Flow: HeLa cells.

Coniugazione

Unconjugated

Grado

Carrier Free, ExactAb™, Validated

Isotipo

Rabbit IgG

SDS-PAGE

150 kDa

Metodo di purificazione

Protein A purified + Peptide affinity purified

Concentrazione

0.5 mg/mL

Product Line

Prodotti biologici

Stoccaggio e spedizione

Forma

Liquid

Condizioni di conservazione di stoccaggio

Store at -20°C,Avoid repeated freezing and thawing

Spedito in

Ice chest + Ice pads

Stabilità e conservazione

Store at 4℃ short term (2 weeks). Store at -20℃ long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Immagini

Western Blot: TOMM40 Antibody (Ab131963)

TOMM40 Antibody (Ab131963) - Western Blot
All lanes: TOMM40 Antibody (Ab131963) at 1/2000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution

Predicted band size: 38 kDa
Observed band size: 38, 39 kDa
Exposure time: 12.9 s

IF/ICC: TOMM40 Antibody (Ab131963)

TOMM40 Antibody (Ab131963) - IF/ICC
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized U2OS (human osteosarcoma cell line) cells labeling TOMM40 with TOMM40 Antibody (Ab131963) at 1/25 dilution, followed by Dylight® 488-conjugated goat anti-rabbit IgG secondary antibody at 1/200 dilution (green). Immunofluorescence image showing mitochondrion staining on U2OS cell line. Cytoplasmic actin is detected with Dylight® 554 Phalloidin at 1/100 dilution (red). The nuclear counter stain is DAPI (blue).

Flow Cytometry: TOMM40 Antibody (Ab131963)

TOMM40 Antibody (Ab131963) - Flow Cytometry
Overlay histogram showing HeLa cells stained with TOMM40 Antibody (Ab131963) (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the TOMM40 Antibody (Ab131963) (1/25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG1 (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.

Applicazione

Applicazione

Dilution info

Flow Cytometry

1/25

WB

1/2000

IF/ICC

1:25

Obiettivi associati (umani)

TOMM40 Tbio Mitochondrial import receptor subunit TOM40 homolog (0 Activities)

Discover Target: TOMM40

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Meccanismi d'azione

Domande frequenti

What does ExactAb? mean?

ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

What does Carrier Free mean?

Carrier Free indicates that the material has been processed and tested for specialty industrial processing. Specifications focus on the impurities that affect performance in that application — for example, metal content for electronics, particle size for batteries, refractive index for optics, or moisture content for moisture-sensitive processes.

How should this product be stored?

Store at ?20 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.

How is this product shipped?

This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (TOMM40 Antibody); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.