ten × Poly (a) polymerase buffer is a matching reaction buffer for poly (a) polymerase and ATP. one × The formula of poly (a) polymer buffer is: 50mm Tris HCl, 250mm NaCl, 1mm DTT, 10mm MgCl2 (pH 8.0 at 25 ℃).
This product is produced with raw and auxiliary materials of medicinal specifications, and all kinds of pollution in the production process are strictly controlled. Product production and quality management procedures in line with GMP specifications ensure that the production process and all raw and auxiliary materials can be traced.
Quality requirements
| project |
standard |
| appearance |
Clear liquid |
| Visible foreign matter |
Compliance with regulations |
| PH value |
7.5-8.5 |
| Endonuclease residues |
Degradation of substrate shall not exceed 10% |
| Exonuclease residues |
Degradation of substrate shall not exceed 10% |
| RNase residue |
Δ CT (Ct sample -Ct negative) not more than 0.5 |
| Bacterial endotoxin content |
≤1EU/ml |
| Mycoplasma detection |
negative |
| Heavy metal residues |
≤10ppm |
|
Follow the following specifications
1. ISO 9001:2015, certified facility。
2. GMP appendix - cell therapy products State Drug Administration.
3. general introduction to human gene therapy - Chinese Pharmacopoeia 2020, National Pharmacopoeia Committee.
4. USP chapter <1043>, adjuvant materials for cell, gene, and tissue engineered products.
5. USP chapter <92>, growth factors and cytokines used in cell therapy manufacturing.
6. Ph. Eur. General chapter 5.2.12, raw materials of biological origin for the production of cell-based and gene therapy medical products.
Product features
For RNA3 ´ end marking.
Poly (a) tails were added to RNA for cloning or affinity purification.
Improve the translation efficiency of RNA in eukaryotic cells.
Storage temperature
-20±5 ℃。
Matters needing attention
1. when using, please mix it upside down gently to avoid foaming, and use it after slight centrifugation. For the preparation and sub packaging of reaction solution, please use new (non RNase contaminated) gun heads, microtubes, etc. to ensure that all reagents are free of RNase contamination.
2. to ensure effective reaction, please operate on ice.
3. for your safety and health, please wear experimental clothes and disposable gloves.
Common reaction system (20μl)
Component
| Dosage
|
RNA | 1-10μg |
10×Poly(A) Polymerase Bufferr, GMP Grade | 2μl |
ATP(10mM) | 1μl |
Poly(A) Polymerase (5U/μl) | 1μl |
Rnase Free Water | 至 20μl |
C2306083