9322-O16B - ≥99% , CAS No.2909427-23-0

CAS: 2909427-23-0 Cat. No.: O1440351 Fórmula: C₄₁H₇₇N₃O₄S₄ Peso molecular: 804.33

GRADE & PURITY ≥99%

Storage

Store at -20°C

Shipped In

Ice chest + Ice pads

Visão geral

9322-O16B, an ionizable cationic lipid, is used for LNP generation.

Specifications

Especificações e pureza

≥99%

Condições de armazenamento de armazenamento

Store at -20°C

Enviado em

Ice chest + Ice pads

Este produto requer transporte de cadeia fria. Serviços terrestres e outros serviços econômicos não estão disponíveis.

Pureza

≥99%

Nomes e identificadores

Peso molecular

804.33

Application Protocols

The following generic protocols are suitable for handling small-molecule library members like 9322-O16B in screening and assay workflows. Adjust according to your assay format and institutional SOPs.

1. Stock solution preparation (general)

• Warm the vial to ambient in a desiccated environment before opening. Accurately weigh the required amount.
• Dissolve in anhydrous DMSO to 10–50 mM. Vortex and, if necessary, sonicate briefly (≤5 min) or gently warm (30–37°C). Avoid prolonged heating.
• Inspect for clarity; if undissolved solids persist, incrementally increase concentration of a secondary miscible solvent (e.g., ACN) or reduce stock concentration.

2. Working solution and dosing

• Prepare intermediate dilutions in DMSO or ACN to facilitate accurate low-volume transfers.
• Dose into buffer/cell media to achieve the final assay concentration, keeping the vehicle constant across controls (typical final DMSO ≤0.5–1% for cells; ≤2–5% for biochemical assays).
• Mix thoroughly (plate shaker 500–800 rpm, 1–3 min). Verify absence of precipitation visually or by light scattering.

3. Plate handling

• Use low-bind tips and plates for hydrophobic compounds. Pre-wet tips with stock solution to minimize adsorption.
• Seal plates to limit evaporation; equilibrate to assay temperature before readout.

4. Stability checks

• For assays >4–6 h or at elevated temperature, confirm stability by re-injecting samples via LC–MS/UV. Protect from strong light if chromophore presence is suspected.

5. Documentation

• Record lot, solvent, stock concentration, and preparation date; link to CoA and any spectral data.

Biological Roles

No biological role or mechanism can be assigned to 9322-O16B from the available product information.

• Item-specific biological function: Not specified for this item; refer to CoA/Spec Sheet.

General context for library compounds (not item-specific)

• Small-molecule library members are typically used as chemical starting points to probe biochemical pathways or phenotypic outcomes in research settings. Any observed activity in assays should be treated as preliminary and confirmed with orthogonal methods and structural analogs to rule out artifacts (e.g., aggregation, redox cycling, fluorescence interference).
• Physicochemical properties (e.g., logD, pKa, solubility) strongly influence apparent biological effects, including cell permeability and nonspecific binding; because these values are unknown here, careful control experiments and concentration-response analyses are essential.

Assay cautions

• Include vehicle controls (matching DMSO percentage), counter-screens for frequent hitters/interferents, and—in cell assays—toxicity readouts to distinguish target-mediated effects from general cytotoxicity.

Research-only notice

• This product is supplied strictly for laboratory research. It is not intended for use in humans, diagnostics, or as a drug substance/excipient without further qualification.

Buffer Applications

This product is a small-molecule library member rather than a buffering reagent; no intrinsic buffering system or pH range is applicable.

• Item-specific buffer role: Not specified for this item; refer to CoA/Spec Sheet.

Practical notes for assay buffers (general guidance)

• Vehicle management: When dosing from DMSO stocks into biological buffers (e.g., PBS, HEPES, Tris), maintain a consistent final DMSO percentage across all wells/samples to avoid solvent-induced effects.
• Precipitation control: Add compound stock last with rapid mixing, or pre-dilute in an intermediate water-miscible organic solvent (e.g., ACN) before addition if allowed, to minimize local supersaturation.
• Surfactants: Low levels of non-ionic surfactants (e.g., 0.01–0.05% Tween-20 or Pluronic F-127) can mitigate aggregation for some hydrophobic compounds; validate that these additives do not interfere with the assay.
• pH and ionic strength: Optimize empirically for target biology; without pKa information for the compound, avoid extreme pH that could induce degradation.

Recommendation

• Verify solubility and stability in the exact assay buffer at working concentrations by quick LC–MS or turbidity/UV checks before large-scale runs.

Green Alternatives

With no structure provided, greener choices are best considered in the context of solvent handling, dissolution, and any follow-on synthesis or purification.

Greener dissolution and assay vehicles (general guidance)

• Prefer ethanol (EtOH) over methanol (MeOH) when feasible for intermediate dilutions; final aqueous systems are typically water-based buffers, minimizing organic content.
• Keep DMSO percentages at the minimum needed for solubility; although DMSO is not highly hazardous, reducing organic solvent load improves EHS profiles.

If synthesis or reformatting is performed (general, literature-based)

• Replace DMF/NMP with safer polar aprotics such as Cyrene or propylene carbonate where compatible with the chemistry.
• Consider 2-MeTHF or CPME as greener ethers instead of THF/diethyl ether for extractions or non-aqueous workups, balancing peroxide risk and recyclability.
• Use water or water/EtOH blends for crystallizations and washes when solubility permits.

Comparison highlights (general)

• DMSO vs DMF: DMSO often offers a better EHS profile and lower volatility; DMF has reproductive toxicity concerns—avoid unless necessary.
• Acetonitrile vs Dichloromethane: ACN has a better environmental and safety profile than chlorinated solvents for many separations.

Operational practices

• Scale solvents to the minimum volume consistent with reliable handling and assay performance.
• Implement solvent recycling where validated by QC.
• Document solvent choices and waste segmentation to support green metrics (e.g., PMI, E-factor).

Note: Specific green substitutions depend on the compound’s functional groups and stability; obtain structural data before changing established methods.

Pharmaceutical Uses

No pharmaceutical or formulation use is specified for 9322-O16B. This product is supplied strictly for research use only.

Item-specific information

• Pharmacopeial status: Not specified for this item; refer to CoA/Spec Sheet.
• Excipient/processing aid use: Not specified for this item; refer to CoA/Spec Sheet.

General context (not item-specific)

• Library compounds at the discovery stage may serve as chemical starting points for structure–activity relationship (SAR) exploration. Transition from research material to any regulated pharmaceutical application requires comprehensive characterization (identity, purity, polymorph, residual solvents), safety assessment, and manufacturing controls under GMP—none of which are asserted or implied for this item.
• If formulation feasibility studies are conducted in vitro, typical vehicles include DMSO stocks diluted into aqueous media, and for pre-formulation research, cosolvent systems or lipid-based carriers may be explored; such activities remain strictly non-clinical and for laboratory investigation only.

Compliance reminder

• Do not use in humans or for diagnostic procedures. Follow institutional and regulatory guidelines for handling, documentation, and disposal of research chemicals.

Physical Properties

The current product data do not include physicochemical specifications for this library member.

Item-specific properties

• Appearance: Not specified for this item; refer to CoA/Spec Sheet.
• Molecular weight: Not specified for this item; refer to CoA/Spec Sheet.
• Molecular formula: Not specified for this item; refer to CoA/Spec Sheet.
• Melting point: Not specified for this item; refer to CoA/Spec Sheet.
• Boiling point: Not specified for this item; refer to CoA/Spec Sheet.
• Density: Not specified for this item; refer to CoA/Spec Sheet.
• Refractive index: Not specified for this item; refer to CoA/Spec Sheet.
• pKa/logP/logD: Not specified for this item; refer to CoA/Spec Sheet.
• Aqueous/organic solubility: Not specified for this item; refer to CoA/Spec Sheet.

General guidance (literature-based, not item-specific)

• For uncharacterized small molecules, initial solubility screening commonly starts with DMSO (analytical grade) at 10–50 mM, followed by dilution into assay buffers with careful control of the final DMSO percentage (typically ≤0.5–1% v/v for cell-based readouts; ≤2–5% v/v for many biochemical assays).
• If crystallinity is observed and dissolution is incomplete in DMSO at room temperature, brief sonication or gentle warming (e.g., 30–37°C) may assist dissolution; avoid prolonged heating unless thermal stability is known.
• Hygroscopicity and polymorphism, when relevant, can affect massing accuracy; equilibrate to room temperature in a desiccated environment before opening.

Please consult the lot-specific CoA/Spec Sheet for definitive physical property values and handling notes.

Quality and Grades

Item-specific grade/purity is not listed in the product data. For definitive specifications, consult the lot-specific CoA/Spec Sheet.

What “grade” typically implies (general guidance)

• Screening/library grade: Usually intended for HTS/HCS and SAR initiation; commonly accompanied by LC–MS purity and, where practical, 1H NMR confirmation. Purity thresholds vary (e.g., ≥90–95% area by LC at a specified wavelength), but are not guaranteed unless stated on the CoA.
• Analytical/HPLC grade (solvents/reagents): Characterized by low UV absorbance, low residue, and stringent contaminants control—mentioned here for context; not applicable unless explicitly stated for this item.

Quality controls commonly applied to library compounds (general; not item-specific)

• Identity: LC–MS (ESI±), HRMS where available; optional NMR (1H/13C) depending on complexity.
• Purity: Gradient LC (UV and/or CAD/ELSD). Reported as area% at specified detection wavelengths.
• Residual solvents/water: Karl Fischer, GC headspace—typically reported on CoA when relevant.
• Salt form/stoichiometry: Clarified in documentation if present (e.g., HCl, TFA, hemisulfate). Impacts MW and dosing.

What is specific to this item

• Grade/purity: Not specified for this item; refer to CoA/Spec Sheet.
• Stabilizers/additives: Not specified for this item; refer to CoA/Spec Sheet.
• Verification: If your workflow requires a particular purity cutoff (e.g., ≥95% UV), please request and review the CoA prior to use.

Recommendations

• Perform in-house verification (quick LC–MS) upon receipt for critical programs.
• For quantitative biology, normalize dosing to free base/acid form once salt form (if any) is known.

Reaction and Applications

No structure-specific reactivity information is available for this item. Therefore, reaction planning and synthetic applications cannot be detailed for 9322-O16B.

General applications for library compounds (non-item-specific)

• Discovery screening: Utilized in high-throughput screening (HTS), high-content screening (HCS), or fragment/lead-like cascades to identify preliminary hits. Data should be confirmed with orthogonal assays and counter-screens.
• SAR development: If hits arise, analog acquisition or synthesis follows, guided by potency, selectivity, and physicochemical optimization (logD, solubility, permeability).
• Mechanism-of-action studies: Post-hit, chemoproteomics or biophysical binding assays (e.g., DSF, ITC, SPR) may be employed to elucidate target engagement, contingent on suitable functionality and solubility.

Practical considerations

• Stock preparation: Generate concentrated DMSO stocks (10–50 mM) with accurate massing, considering potential salt forms indicated on the CoA.
• Adsorption and carryover: Minimize compound loss by using low-bind plastics and consistent pipetting protocols, particularly for hydrophobic or cationic species.
• Stability checks: For multi-day assays, confirm stability of working solutions by LC–UV/MS at assay-relevant conditions (temperature, pH, light exposure).

If synthetic use is intended

• Obtain the structure and any protecting groups/salt forms from the CoA. Reaction conditions, functional group compatibility, and workup cannot be recommended without structural information.

Reaction Conditions

Reaction conditions specific to 9322-O16B cannot be recommended in the absence of structural details.

General guidance (not item-specific)

• Solvent and temperature: Choose based on functional group demands (e.g., polar aprotics for nucleophilic substitutions; alcoholic or aqueous media for ionic processes). Start with moderate temperatures (20–60°C) and adjust as stability allows.
• Catalysts: Selection (e.g., Pd, Cu, Ni for cross-couplings; Brønsted/Lewis acids for additions) depends on the presence of coupling handles or activating groups.
• Atmosphere: Use inert gas (N2/Ar) where air or moisture sensitivity is possible; rigorously dry glassware/solvents for organometallic chemistry.
• Workup: Favor greener extractions (2-MeTHF/EtOAc) and buffered aqueous washes; avoid extreme pH until hydrolytic stability is known.
• Analytical monitoring: LC–MS and UHPLC-UV provide rapid insight into conversion, byproducts, and stability windows.

Assay preparation context

• For biological testing, prepare DMSO stocks (10–50 mM), verify clarity after buffer dilution, and maintain consistent vehicle controls. Confirm short-term stability at assay temperature by re-injecting samples where feasible.

Please consult the CoA/Spec Sheet for any item-specific restrictions, sensitivities, or recommended handling conditions if they are provided with your lot.

Safety and Handling

GHS and hazard details for this specific item are not provided in the listing and may vary by lot. Always consult the product SDS for authoritative safety information.

Item-specific hazard data

• Signal word: Not specified for this item; refer to SDS.
• GHS classification: Not specified for this item; refer to SDS.
• Pictograms: Not specified for this item; refer to SDS.
• H-statements: Not specified for this item; refer to SDS.

General laboratory precautions for small-molecule solids

• PPE: Use lab coat, safety glasses, and appropriate chemically resistant gloves (e.g., nitrile). Avoid inhalation of dusts and skin/eye contact.
• Engineering controls: Handle powders in a fume hood or ventilated enclosure to minimize aerosol generation. Use antistatic measures for microgram–milligram weighing.
• Storage compatibility: Store segregated from strong oxidizers and acids/bases unless the specific structure dictates otherwise. Keep container tightly closed, dry, and protected from light until structural photolability is known.
• Spill/accidental release: Avoid dust formation. Collect with inert absorbent or dampened wipes. Dispose according to institutional chemical waste procedures.
• First aid (overview; defer to SDS): If inhaled—fresh air; seek medical advice. Skin/eye contact—rinse with water for 15 minutes; remove contaminated clothing; seek medical advice. If ingested—rinse mouth; do not induce vomiting; seek medical attention.

Special risks

• Unknown sensitization or bioactivity: Treat as potentially bioactive; minimize exposure and avoid aerosolization.
• Thermal stability/peroxide formation: No item-specific risks indicated; do not heat above ambient without confirming stability.

Waste

• Collect solid and DMSO solutions as halogenated/non-halogenated organic waste per local regulations and your EHS guidance.

Solvent Selection

Because the molecular structure is not provided, solvent choice should begin with broadly compatible, high-boiling polar aprotic vehicles and then be tailored empirically.

Initial dissolution strategy (general guidance)

• Primary vehicle: DMSO (analytical grade) due to its broad solvating power and assay compatibility at low percentages.
• Secondary options: DMF, NMP, acetonitrile, methanol, ethanol. For hydrophobic entities, include co-solvent blends (e.g., DMSO + acetonitrile) or add non-ionic surfactants at low levels for biological assays if permitted.
• Aqueous dilution: Dilute concentrated organic stock into buffer/medium with vigorous mixing to prevent precipitation; control final DMSO ≤0.5–1% v/v for cells and ≤2–5% v/v for many biochemical assays.

Useful solvent properties (literature; general, not item-specific)

• DMSO: dielectric ~47; bp 189°C; miscible with water and organics; excellent solvency but can enhance membrane permeability—use low assay percentages.
• DMF: dielectric ~37; bp 153°C; strong solvent; higher toxicity; prefer DMSO when possible.
• Acetonitrile: dielectric ~37; bp 82°C; miscible with water; lower viscosity; good for LC and serial dilutions.
• Methanol/Ethanol: protic; facilitate ionizable compounds; often preferred for greener profiles (especially ethanol).

Practical tips

• Prepare 10–50 mM DMSO stocks; sonicate briefly if needed. Filter through 0.2 µm PTFE only if chemically compatible and adsorption is acceptable.
• For plate-based screening, pre-wet tips with DMSO stocks to reduce adsorption; use low-bind labware for sticky compounds.
• If precipitation occurs post-dilution, increase temperature slightly, extend mixing, or adjust organic percentage within assay limits.

Storage and Reconstitution

Item-specific storage conditions are provided for this product; additional best practices are included below for robust handling of small-molecule library members.

Item-specific instructions

• Storage temperature: Store at -20°C.
• Shipped in: Ice chest + Ice pads.

General storage guidance (not item-specific)

• Keep the container tightly closed in a dry, inert atmosphere. Allow the vial to equilibrate to room temperature in a desiccator before opening to prevent moisture condensation.
• If the compound is moisture- or light-sensitive (unspecified here), consider storing under inert gas and protecting from light (amber vials, foil wrap).
• Avoid repeated freeze–thaw cycles. Upon first use, prepare single-use aliquots of DMSO stock (e.g., 10–50 mM) in low-bind, airtight vials or plates. Store aliquots at -20°C to -80°C as permitted by stability data.
• Record date of dissolution; many small molecules are stable for weeks to months at -20°C in dry DMSO, but confirm with stability checks relevant to your application.

Reconstitution (general)

• Solvent: Start with anhydrous DMSO. If required, use co-solvents (ACN, EtOH) or formulate with minimal surfactant compatible with the assay.
• Procedure: Vortex, brief sonication, and gentle warming (30–37°C) as needed. Filter through 0.2 µm PTFE only if validated for adsorption losses.

Disposal

• Dispose of remaining material and solutions as organic chemical waste per institutional and local regulations.

Always refer to the lot-specific CoA and SDS for definitive, item-specific handling and stability information.

Structure and Identity

Overview: This catalog entry corresponds to a small-molecule library member supplied for research screening and discovery workflows. Structural specifics are not provided in the current product data.

• SKU: O1440351
• Product Name: 9322-O16B
• CAS: 2909427-23-0
• Category: Small molecules and compound libraries (全部 / 可售 / 小分子和化合物库)
• Research use: For research use only

Item-specific identifiers

• Molecular formula: Not specified for this item; refer to CoA/Spec Sheet.
• Molecular weight: Not specified for this item; refer to CoA/Spec Sheet.
• SMILES: Not specified for this item; refer to CoA/Spec Sheet.
• InChIKey: Not specified for this item; refer to CoA/Spec Sheet.

Structural description

• 2D/functional features: Not available from the listing. If required for cheminformatics ingestion (e.g., for screening cascade design or PAINS filtering), please request the structure file (SDF/Molfile) or see the CoA if provided with shipment.

Notes for informatics integration (general guidance)

• Without SMILES/InChIKey, internal databases can register this entry by CAS (2909427-23-0) and supplier SKU as provisional identifiers pending full structure disclosure.
• If the compound is part of a plated library, the plate map and well position metadata (if supplied) should be retained to ensure traceability across HTS/assay campaigns.

Provenance and traceability

• For lot-specific structure confirmation and purity, defer to the Certificate of Analysis (CoA) and any accompanying LC–MS/NMR data shipped with the product.

Synthetic Utility

Synthetic applications for 9322-O16B cannot be described without structural information.

General considerations for using library compounds in synthesis (not item-specific)

• If repurposing a screening hit as a synthetic intermediate or scaffold, obtain the full structure, stereochemistry, and salt/solvate form from the CoA or supplier. Confirm by LC–MS and NMR before scale-up.
• Protecting group and functional group compatibility dictate route design; evaluate stability to acids/bases, nucleophiles/electrophiles, and redox conditions by small-scale probes.
• For analog generation, identify modular points (e.g., aryl halides, boronics, amines, acids, alcohols) to enable cross-coupling, amide coupling, SNAr, or reductive amination—strategy depends entirely on the actual functionality present.

Operational tips

• If solubility is limited, switch to higher-boiling polar aprotic solvents or biphasic couplings (e.g., aqueous base + organic phase with PTC) consistent with the compound’s stability.
• Monitor reactions by LC–MS to distinguish desired transformations from degradation, especially if unknown weak points (e.g., benzylic oxidation, hydrolysis-prone linkages) may exist.

Conclusion

• Please obtain and review the structural dossier (SDF, spectral data) prior to any synthetic use; without it, only generic guidance is possible.

Target Specificity

No target or selectivity information is provided for 9322-O16B.

• Item-specific target data: Not specified for this item; refer to CoA/Spec Sheet.

General notes (not item-specific)

• In discovery campaigns, initial hits require confirmation, counter-screening against related targets, and determination of off-target profiles (e.g., by broad panel screening) to establish meaningful selectivity.
• Chemotype-dependent assay interferences (e.g., redox cycling, colloidal aggregation, fluorescence quenching/auto-fluorescence) should be evaluated early to avoid false positives.

Recommendation

• If target information becomes available, construct a profiling panel including orthologs/isoforms and representative antitargets. Use orthogonal binding/functional assays to triangulate specificity.

Perguntas frequentes

What is the purity of this product?

This product is supplied at ≥99% purity (chemical assay). Lot-specific values are stated on the Certificate of Analysis.

How should this product be stored?

Store at ?20 °C. Freezer storage is required to maintain the specified shelf life.

How is this product shipped?

This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.

What are the CAS number, molecular formula and molecular weight?

The CAS Number is 2909427-23-0, the molecular formula is C41H77N3O4S4, and the molecular weight is 804.33 g/mol.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.