≥98% for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
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Storage & shipping
Protected from light,Store at -80°C Ships Dry ice packs + Cold packs Check lot-specific COA for exact specifications.
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Quality documents
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
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Literature proof
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Visão geral
ACH-000143 is a potent and orally active melatonin receptor agonist, with EC 50 values of 0.06 nM and 0.32 nM for MT1 and MT2 , respectively
In Vivo
ACH-000143 reduces liver triglycerides and steatosis in diet-Induced obese rats . ACH-000143 is devoid of hERG binding, genotoxicity, and behavioral alterations at doses up to 100 mg/kg p.o., supporting further investigation of this compound as a drug candidate . ACH-000143 significantly reduces plasma glucose at 10 mg/kg (−16.4%, p < 0.05) and 30 mg/kg (−16.9%, p < 0.01) . MCE has not independently confirmed the accuracy of these methods. They are for reference only. Animal Model: High-fat diet rats . Dosage: 10 and 30 mg/kg. Administration: Orally, once daily for two months. Result: Significantly reduced the weekly BW gain on weeks.
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Revisões
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Application Protocols
Item-specific, validated application protocols are not provided in the Product Data for ACH-000143.
General screening workflow (literature/general)
Stock preparation: Dissolve in anhydrous DMSO to 10–50 mM; verify complete dissolution (visual inspection, light scattering) and sterile-filter if compatible (0.2 μm PTFE).
Plating: Use acoustic or tip-based transfer to assay plates; maintain uniform DMSO across wells. Include blank DMSO controls and known positive/negative controls relevant to the assay technology.
Concentration–response: Generate 8–12 point serial dilutions (e.g., 1:2 or 1:3) to estimate potency metrics (IC50/EC50) and Hill slope; confirm activity by orthogonal assay.
Data integrity: Monitor for aggregation (detergent controls), redox cycling, fluorescence interference (spectral scans), and metal chelation artifacts as appropriate.
Note
For any specific application supplied by the end-user or as released by the manufacturer, defer to the detailed protocol and SDS/CoA for ACH-000143.
Biological Roles
Item-specific biological functions, targets, or pathway roles are not provided in the Product Data for ACH-000143.
General context (literature/general)
Small-molecule library members serve as chemical probes or starting points for discovering modulators of enzymes, receptors, transporters, or signaling pathways. Their biological roles are established empirically through screening and follow-up assays.
Without a disclosed structure or known target, no assertions can be made regarding mechanism, on/off-target profiles, permeability, or metabolic liabilities for this item.
Practical recommendations
Use unbiased profiling (e.g., broad panel biochemical screens, phenotypic assays) followed by orthogonal confirmation to define any biological role for ACH-000143.
Employ standard liability assays (cytotoxicity counterscreens, redox interference, aggregation, and assay-technology interference tests) to avoid false positives.
Compliance note
For research use only (Product Data). No medical, diagnostic, or therapeutic applications are implied.
Buffer Applications
Not typically applicable. ACH-000143 is a small-molecule screening compound rather than a buffering agent.
General guidance (literature/general)
When preparing assay media containing small-molecule stocks, choose buffers compatible with the biological system (e.g., PBS, HEPES, Tris) and maintain DMSO at the lowest possible final percentage to prevent buffer–compound precipitation or assay interference.
Adjust pH after compound addition only if the compound’s ionization is known and sensitive to pH; no pKa data are available for this item.
Item-specific buffer usage details (pH range, capacity, recipes): Not specified for this item; refer to CoA/Spec Sheet if provided.
Green Alternatives
This section addresses solvent and process choices surrounding the handling of small-molecule screening compounds in a greener laboratory context. No structure-specific hazards are provided for ACH-000143, so guidance is general.
Preferred solvents for weighing/dissolution: Water where feasible; otherwise, bio-based or improved EHS solvents such as ethanol (renewable), ethyl acetate, or 2-MeTHF for non-aqueous work. DMSO remains widely used for stocks due to assay compatibility; use minimal volumes and recover via solvent consolidation programs.
Energy efficiency: Store only essential aliquots at −80 °C; keep working aliquots at −20 °C if stability supports it (verify experimentally) to reduce ultra-cold burden.
Waste reduction: Prepare small-volume, on-demand stocks to minimize disposal of expired materials; segregate halogenated vs non-halogenated waste for optimal treatment.
Illustrative comparison (general)
DMSO vs ethanol for initial dissolution
DMSO: High solvency, excellent assay compatibility; not renewable, but low volatility reduces emissions.
Ethanol: Renewable, lower toxicity; may not dissolve highly lipophilic compounds and can impact assays at modest percentages.
Note
Any switch to greener solvents or milder storage must be confirmed by stability and activity testing for ACH-000143, as item-specific data are not provided.
Pharmaceutical Uses
Not applicable as a marketed excipient or active pharmaceutical ingredient based on the Product Data provided.
General context (literature/general)
Screening compounds may inform discovery programs but are not formulated for clinical use. They generally lack pharmacopeial monographs, GMP documentation, or validated impurity/stability profiles required for manufacturing.
Item-specific regulatory status
For research use only (Product Data). No claims regarding therapeutic use, dosage forms, or clinical applications.
Physical Properties
Item-specific (Product Data)
Appearance: Not specified for this item; refer to CoA/Spec Sheet.
Molecular Weight: Not specified for this item; refer to CoA/Spec Sheet.
Typical physicochemical parameters (literature/general guidance; not item-specific)
Melting point (MP), boiling point (BP), density, logP, pKa, and refractive index are commonly reported for discrete small molecules; however, no such values are provided for ACH-000143 in the Product Data.
Solubility guidance (general): Small-molecule library members are often soluble in high-purity DMSO at 10–50 mM stock concentrations; aqueous solubility, if any, depends on ionizable groups and logP but is not reported here.
What is known vs unknown for this item
BP/MP: Not specified for this item; refer to CoA/Spec Sheet.
Density: Not specified for this item; refer to CoA/Spec Sheet.
Solubility: Not specified for this item; refer to CoA/Spec Sheet (typical screening stocks use anhydrous DMSO).
logP/logD: Not specified for this item; refer to CoA/Spec Sheet.
pKa: Not specified for this item; refer to CoA/Spec Sheet.
UV-Vis characteristics: Not specified for this item; refer to CoA/Spec Sheet.
Practical note (general)
In absence of a structure, pre-screen small-scale dissolutions in DMSO and, if needed, co-solvents (e.g., DMF, ethanol) and verify clarity/absence of precipitate prior to assay plating.
Quality and Grades
Item-specific quality information
Grade/Purity: Not specified for this item; refer to CoA/Spec Sheet.
Appearance: Not specified for this item; refer to CoA/Spec Sheet.
General guidance on grades for small-molecule screening compounds (literature/general)
Screening/Library grade: Typically supplied at high chemical purity suitable for in vitro discovery workflows (e.g., biochemical and cell-based assays). Exact purity thresholds, residual solvent limits, and spectral identity criteria vary by supplier and are specified on the CoA.
Analytical verification: Identity commonly supported by NMR and/or LC–MS. HPLC purity reporting (area%) is typical for screening compounds; enantiomeric excess (ee) may be provided for chiral items when relevant.
Stabilizers: If present, will be declared on the label/CoA. No stabilizer information is provided for this item.
What to check on receipt (practical tips)
Review CoA for: HPLC purity, identity confirmation (LC–MS/NMR), water content (if applicable), residual solvents, and storage recommendations.
Inspect container integrity after dry-ice shipment. Equilibrate to room temperature in a desiccator before opening to minimize condensation. Re-cap promptly to limit moisture/oxygen exposure.
Regulatory/traceability note
For research use only (Product Data). Not for human or veterinary use.
Reaction and Applications
Item-specific reactivity/application details are not supplied in the Product Data for ACH-000143.
General research applications for small-molecule library members (literature/general guidance)
High-throughput screening (HTS) and fragment/lead discovery: Compounds are typically evaluated across biochemical targets, cell-based phenotypic assays, and counter-screens to establish activity and selectivity profiles.
Mechanistic probes: Upon activity detection, orthogonal assays (e.g., SPR, DSF, ITC) are employed to assess binding; structure–activity relationship (SAR) is developed with analog series.
Chemical biology: Hit compounds may be derivatized with reporter tags (biotin, fluorophores) for target engagement assays, provided functional handles exist (unknown here).
Practical notes
Plate handling: Dispense DMSO stocks using low-retention tips; maintain final assay DMSO ≤0.5–1% v/v unless protocol supports higher.
Stability checks: Run forced-degradation snapshots (light, pH, oxidants) at micro-scale to confirm assay stability in relevant media.
Control selection: Include pan-assay interference compound (PAINS) filters and redox/aggregation controls (e.g., detergents, counterscreens) during primary screening.
Limitations
Without structural information, specific reaction chemistries (e.g., cross-coupling handles, nucleophilicity/electrophilicity) cannot be detailed for this item.
Reaction Conditions
Item-specific reaction conditions are not available due to the absence of structural information in the Product Data.
General guidance (literature/general)
Solvent systems: DMSO, DMF, MeCN, and mixed aqueous/organic media are common in small-molecule manipulations; selection depends on functional groups and desired chemistry.
Temperature: Many polar aprotic-mediated reactions proceed at 20–80 °C; sensitive transformations may require 0–10 °C or photochemical conditions.
Catalysts and bases: Palladium or nickel for cross-coupling; tertiary amines or inorganic bases for substitutions; photoredox catalysts for radical functionalization—applicability depends entirely on structure.
Time and monitoring: TLC, LC–MS, and HPLC are standard for tracking conversions; quench and workup conditions should be tailored to stability (unknown here).
Assay usage conditions (screening; general)
DMSO stock concentrations: commonly 10–50 mM; final assay concentration depends on screening design (e.g., single-point 10 μM with follow-up titration).
Light/oxidation sensitivity: Store and handle protected from light (Product Data). Consider inert atmosphere and antioxidant screens during method development if instability is suspected.
Safety and Handling
Safety summary for this item (from Product Data)
GHS Classification: Not specified for this item; refer to SDS.
Signal Word: Not specified for this item; refer to SDS.
H-Statements: Not specified for this item; refer to SDS.
Pictograms: Not specified for this item; refer to SDS.
Storage and shipping (Product Data)
Storage: Store at −80 °C, protected from light.
Shipped In: Dry ice packs + cold packs.
General laboratory precautions (literature/general guidance)
Handle unknown small molecules in a chemical fume hood with appropriate PPE: lab coat, safety glasses, and suitable gloves (e.g., nitrile). Avoid inhalation, ingestion, and skin contact.
Minimize repeated freeze–thaw of DMSO stocks to reduce degradation and contamination.
Incompatibilities cannot be defined without structure; as a conservative practice, segregate from strong oxidizers and acids/bases until specific data are available.
First-aid overview (general)
Skin/eye contact: Rinse with water for several minutes; remove contaminated clothing; seek medical attention per SDS guidance.
Inhalation: Move to fresh air; seek medical attention if symptoms occur.
Ingestion: Rinse mouth; do not induce vomiting; seek medical attention.
Authoritative reference
Always consult the Safety Data Sheet (SDS) for ACH-000143 for definitive hazard classification, response measures, and disposal guidance.
Solvent Selection
Item-specific solvent information
Solubility/miscibility: Not specified for this item; refer to CoA/Spec Sheet.
General solvent strategy for small-molecule library compounds (literature/general)
Primary stock solvent: Anhydrous DMSO is standard for discovery screening stocks (e.g., 10–50 mM). Verify complete dissolution by gentle vortex and short sonication if needed.
Alternative solvents: If DMSO solubility is inadequate, trial DMF, ethanol, isopropanol, or mixtures with aqueous buffers containing minimal co-solvent (≤1–2% v/v) for assays.
Aqueous compatibility: Depends on ionizable groups (unknown here). Titrate pH with matched volatile buffers (e.g., HEPES, PBS) and add surfactant (0.01–0.05% Tween-20) sparingly if precipitation occurs in biological media.
Comparison notes (general)
DMSO vs DMF: DMSO is preferred for biological compatibility; DMF can enhance solubility but is less favored in cell assays.
Alcohols: Ethanol/isopropanol can assist solubilization but may affect assay readouts at >0.5–1% v/v.
Practical tip
Prepare a small solubility matrix (DMSO, DMF, EtOH) at micro-scale to identify a clear, stable stock. Filter (0.2 μm PTFE) if particulates are observed, provided no adsorption losses occur.
Storage and Reconstitution
Item-specific (Product Data)
Storage Conditions: Store at −80 °C and protect from light.
Shipping: Dry ice packs + cold packs.
Item-specific values not provided
Recommended diluent/solvent: Not specified for this item; refer to CoA/Spec Sheet.
Concentration of stock solutions: Not specified for this item; refer to CoA/Spec Sheet.
General guidance for small-molecule screening compounds (literature/general)
Reconstitution: Prepare stocks in anhydrous DMSO (common working range 10–50 mM). If solubility is limited, consider DMF or ethanol, or co-solvent mixtures validated for your assay.
Aliquoting: Dispense single-use aliquots (e.g., 10–50 μL) into low-binding vials or plates to minimize freeze–thaw. Flush headspace with inert gas if oxidation-sensitive.
Light protection: Use amber vials or foil wrap during handling and storage, consistent with Product Data.
Stability checks: Periodically confirm identity/purity by LC–MS or HPLC after storage, especially if stored >6–12 months or subjected to multiple temperature cycles.
Thawing: Warm sealed containers to ambient temperature before opening to avoid moisture condensation; promptly re-seal after use and return to −80 °C.
Waste and disposal
Dispose of unused solutions and materials according to institutional chemical waste procedures and SDS guidance.
Structure and Identity
Brief overview: ACH-000143 is listed as a small-molecule library member. Structural identifiers are not provided in the Product Data.
Item-specific (Product Data)
SKU: A650590
Product Name: ACH-000143
CAS: 2225836-30-4
PubChem CID: 139451790
InChIKey: 35570 (as provided; note: this does not match standard InChIKey formatting)
SMILES: Not specified for this item; refer to CoA/Spec Sheet.
Molecular Formula: Not specified for this item; refer to CoA/Spec Sheet.
Molecular Weight: Not specified for this item; refer to CoA/Spec Sheet.
Structural features
Not specified in Product Data. Without a validated SMILES/InChI or structure, functional groups, ring systems, and stereochemistry cannot be summarized for this listing.
2D structure description (general note)
Not available from Product Data. Please consult the Certificate of Analysis (CoA), Spec Sheet, or the supplier’s structure disclosure (if applicable) for a definitive 2D representation.
Synthetic Utility
Item-specific synthetic transformations cannot be detailed because no structure or functional group information is provided for ACH-000143.
General considerations for small-molecule library members (literature/general)
If structural information becomes available, typical synthetic utilities may include:
Diversification via cross-coupling (e.g., Suzuki–Miyaura, Buchwald–Hartwig) if aryl halides/boronates/amines are present.
Late-stage functionalization (C–H activation, photoredox) to access SAR analogs.
Introduction of reporter handles (alkynes/azides) for click chemistry in target engagement assays.
Protecting-group strategies, chemo-/regioselectivity, and stereocontrol are driven by the specific functional group set; none can be asserted here without structural data.
Practical tip
Retain a small portion of the original material for spectral re-verification (LC–MS/NMR) if synthetic elaboration is attempted, and archive spectra with each round of derivatization.
Target Specificity
Not applicable. ACH-000143 is not an antibody, protein, or targeted biological reagent in this listing.
Item-specific target data
No target, enzyme, receptor, or pathway specificity is provided in the Product Data.
General note (literature/general)
Any putative target specificity for a screening compound must be established empirically via biochemical assays, binding studies, and orthogonal validation; none is provided here.
Perguntas frequentes
What is the purity of this product?
This product is supplied at ≥98% purity (chemical assay). Lot-specific values are stated on the Certificate of Analysis.
How should this product be stored?
Store at ?80 °C, protected from light. The material is light-sensitive — keep it in its original opaque or amber container.
How is this product shipped?
This product ships frozen with dry ice and cold packs. Unpack on arrival and transfer it to the storage condition stated above; handle dry ice with insulated gloves in a ventilated area.
What are the CAS number, molecular formula and molecular weight?
The CAS Number is 2225836-30-4, the molecular formula is C13H16ClN3O3, and the molecular weight is 297.74 g/mol. InChIKey BVSLRGAPRGQWNC-UHFFFAOYSA-N.
What documentation is provided?
Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.
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