Alizarin Red S Staining Solution (0.1%) - BioReagent,Biological Stain,for microscopy,0.1% , CAS No.130-22-3

CAS: 130-22-3 Cat. No.: A774864 Fórmula: C₁₄H₇NaO₇S Peso molecular: 342.26 Beilstein Registry Number: 4117090 Número CE: 204-981-8

GRADE & PURITY BioReagent, Biological Stain, for Microscopy, 0.1%

Synonyms

ARS Stain

Storage

Protected from light,Room temperature

Shipped In

Normal

Visão geral

Calcium is abundant in the human body, forming the skeleton that serves as the supporting framework. It also plays important roles in secretion, transport, muscle contraction, and nerve conduction. Calcium exists in the body in two forms: one is ionic calcium, present in the bloodstream and referred to as blood calcium; the other is bound calcium, which combines with proteins, carbonates, or phosphates and deposits in tissues. Aside from bones and teeth, calcium normally permeates all tissues and cells and generally does not appear as a solid within tissues. However, under certain conditions, calcium may precipitate as a solid and deposit in tissues, leading to pathological calcification. The deposited calcium salts are primarily calcium phosphate, followed by calcium carbonate. Calcium salts are usually monorefringent, but calcium oxalate is birefringent. When stained with HE, calcium typically appears purplish-blue. Many dyes can form chelates with calcium, including Alizarin Red S, purpurin, nuclear fast red, and others.

Alizarin Red S staining is a commonly used staining method widely employed in the study of osteoblast differentiation, bone cells, or tissue pathophysiology. Alizarin Red S (abbreviated as ARS), also known as sodium alizarinsulfonate or alizarin carmine, has a CAS number of 130-22-3, molecular formula C₁₄H₇NaO₇S, and molecular weight 342.25. Alizarin Red S is an anthraquinone derivative that can chelate with various metal ions to form stable orange-red or deep red complexes. It serves as an excellent metal indicator, a chromogenic agent for spectrophotometric determination of metal ions, an acid-base indicator, and is used in polarographic determination of metal ions. In histology and histopathology, because Alizarin Red S can chelate with calcium salts in calcium carbonate or calcium phosphate to form an orange-red complex, it is often used to stain or localize calcium deposits in tissues.

Instructions for Use

1. Fix tissue in 10% neutral formalin or ethanol, followed by routine dehydration and embedding.

2. Section to 95% ethanol.

3. Place slides upright and allow to dry completely.

4. Immerse sections in a staining jar containing Alizarin Red S staining solution for 5–20 minutes (see Note 1).

5. Rinse quickly with distilled water.

6. (Optional) Counterstain with a counterstaining solution, then rinse with distilled water three times.

7. Routine dehydration and clearing, mount with neutral balsam.

Staining Results

Calcium deposits	Orange‑red
Background	Varies depending on the counterstain used

Notes

1. The staining time with Alizarin Red S should be determined based on the calcium salt content. Observe under a microscope and remove the slide for washing when calcium salts appear a deep orange‑red. Over‑staining may cause diffusion; typically, 1–2 minutes is sufficient.

2. After staining with Alizarin Red S, calcium deposits are birefringent.

3. The Alizarin Red S method is particularly useful for identifying and detecting small amounts of calcium, such as examining abnormal calcification in the kidneys (hypercalciuria).

4. For your safety and health, please wear a lab coat and disposable gloves during operation.

Specifications

Sinónimos

ARS Stain

Especificações e pureza

BioReagent,Biological Stain,for microscopy,0.1%

Estabilidade e armazenamento

Store at room temperature long term (12 months). Store in the dark.

Condições de armazenamento de armazenamento

Protected from light,Room temperature

Enviado em

Normal

Grau

Biological Stain, BioReagent, for Microscopy

Nomes e identificadores

• SMILES isoméricas: C1=CC=C2C(=C1)C(=O)C3=CC(=C(C(=C3C2=O)O)O)S(=O)(=O)[O-].[Na+]
• WGK Alemanha: 3
• RTECS: CB1095300
• Peso molecular: 342.26
• Beilstein: 4117090
• Reaxy-Rn: 4117090
• Reaxys-RN_link_address: https://www.reaxys.com/reaxys/secured/hopinto.do?context=S&query=IDE.XRN=4117090&ln=

Propriedades químicas e físicas

• Sensibilidade: Light-sensitive

Perguntas frequentes

What does Biological Stain mean?

Biological Stain indicates that the product has been processed and tested for biological and biochemical applications. Specifications may include endotoxin limits, microbial controls, sterility, low DNase/RNase/protease activity, and verified biological activity where relevant.

What does BioReagent mean?

BioReagent indicates that the product has been processed and tested for biological and biochemical applications. Specifications may include endotoxin limits, microbial controls, sterility, low DNase/RNase/protease activity, and verified biological activity where relevant.

How should this product be stored?

Store at room temperature, protected from light. The material is light-sensitive — keep it in its original opaque or amber container.

How is this product shipped?

This product ships under standard ambient conditions. No temperature-controlled packaging is required.

What are the CAS number, molecular formula and molecular weight?

The CAS Number is 130-22-3, the molecular formula is C14H7NaO7S, and the molecular weight is 342.26 g/mol.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.