AZ82 is a selective kinesin-like protein KIFC1 (HSET/KIFC1) inhibitor, with a K i of 43 nM and an IC 50 of 300 nM for KIFC1.
In Vitro
AZ82 is shown to specifically induce multipolar spindles in BT-549 cells, but not in cancer cells with normal centrosome number, such as HeLa. AZ82 binds specifically to KIFC1/MT complex but not to KIFC1 or MT alone. Treatment with AZ82 caused centrosome declustering in BT-549 breast cancer cells with amplified centrosomes. AZ82 inhibits both processes with an IC 50 of 0.90 ± 0.09 μM for mant-ATP binding and 1.26 ± 0.51 μM for mant-ADP releasing. MCE has not independently confirmed the accuracy of these methods. They are for reference only.
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Application Protocols
Item-specific
No validated application protocols (e.g., assay types, dilutions, conditions) are provided for this item. Refer to your internal SOPs or the primary literature when designing experiments involving AZ82.
General best practices (non-validated, for planning only)
Prepare concentrated DMSO stock (e.g., 10 mM), aliquot, and store at −80°C to minimize freeze–thaw.
Establish vehicle controls and a serial dilution scheme (e.g., 1:3 or 1:2) for concentration–response curves across a relevant range.
Confirm compound integrity by LC–MS before and after key studies to document stability.
Biological Roles
Item-specific
No target, pathway, or MoA information is provided in the Product Data for AZ82. This product is supplied strictly for research use only.
General/literature context (no therapeutic claims)
Compounds in small-molecule libraries are typically used as chemical probes or tool compounds to modulate biological pathways in vitro and in cell culture, enabling studies of signaling, transport, metabolism, or organelle function.
Researchers commonly validate biological roles by combining: structure–activity relationship (SAR) analysis, genetic perturbations (e.g., CRISPR/siRNA), and orthogonal assays (biochemical vs cellular readouts) to triangulate on-target effects.
When using any compound identified by a code name (e.g., AZ82), confirm identity and reported function via primary literature and match CAS/CID to avoid confusion with similarly named entities.
Good practices
Establish concentration–response relationships (e.g., EC50/IC50 determination) with appropriate vehicle controls.
Evaluate selectivity by screening against a panel of related targets and assess cytotoxicity independently from pathway readouts to exclude false positives.
Buffer Applications
Applicability
AZ82 is not a buffering agent and is not used to set or maintain pH. Accordingly, there are no canonical buffer recipes or buffering ranges associated with this compound.
Practical notes for assay buffers (general)
When dosing bioactive small molecules into buffers, choose systems that maintain stability and target activity (e.g., HEPES or PBS for many cell-based assays). Confirm that the buffer components do not react with or sequester the compound.
Maintain consistent organic cosolvent content (e.g., DMSO ≤0.1–0.5% v/v) across control and treated samples to avoid vehicle-induced artifacts.
Green Alternatives
Relevance to this product
AZ82 is a discrete bioactive small molecule, not a process solvent or bulk reagent. Therefore, selection of a “green alternative” typically does not apply in the way it does for solvent choice or commodity reagents.
General sustainability considerations for small-molecule screening
Solvent minimization: Use high-concentration DMSO stocks to reduce solvent volumes across screens. Consolidate plate maps to minimize dead volume and waste.
Greener solvents for formulation: Where compatible, favor ethanol or aqueous vehicles over higher-toxicity aprotic polar solvents for intermediate dilutions.
Waste management: Segregate DMSO- and DMF-containing waste and apply microscale assay formats (1536-well where feasible) to curb chemical consumption.
Packaging and logistics
Choose aliquot sizes that match experimental throughput to limit freeze–thaw and reduce spoilage waste. Coordinate consolidated shipping on dry ice to reduce logistic emissions when possible.
Pharmaceutical Uses
Applicability
No pharmacopeial status, excipient role, or formulation use is specified for this item. AZ82 is provided for research use only and is not intended for human or veterinary use.
General considerations (non-clinical)
In preclinical research, tool compounds may be formulated for in vitro or exploratory in vivo studies to probe pharmacology. Any such use requires verification of identity, purity, and stability, along with appropriate safety approvals. This catalog entry does not provide such specifications; consult the CoA/SDS and institutional guidelines.
Physical Properties
Item-specific (from Product Data)
Appearance: Not specified for this item; refer to CoA/Spec Sheet.
Measured/specification values for this catalog item
Boiling point: Not specified for this item; refer to CoA/Spec Sheet.
Melting point: Not specified for this item; refer to CoA/Spec Sheet.
Density: Not specified for this item; refer to CoA/Spec Sheet.
Refractive index: Not specified for this item; refer to CoA/Spec Sheet.
Water content, residual solvents, metal content, UV cutoff: Not specified for this item; refer to CoA/Spec Sheet.
Not provided here due to incomplete structural information. Consult primary literature or validated databases using CAS 1449578-65-7 / CID 91885438 for typical physicochemical data (e.g., cLogP, pKa, solubility class) if required for method development.
Practical notes (general)
Many library-grade small molecules are hydrophobic solids supplied for dissolution in anhydrous DMSO to prepare 10–50 mM stocks, then diluted into aqueous media with final DMSO ≤0.1–0.5% v/v for cell assays. Verify actual solubility empirically for AZ82 once the structure and purity lot data are in hand.
Quality and Grades
Item-specific
Grade/purity: Not specified for this item; refer to CoA/Spec Sheet.
How to interpret grades (general guidance)
Screening/library grade: Typically intended for biochemical/cellular assays; emphasis on identity confirmation (1H NMR/HRMS) and assay-compatible purity (e.g., ≥95% by HPLC). Exact thresholds vary by supplier and must be confirmed on the lot-specific CoA.
Analytical/trace grades: Specify narrow limits on residual solvents, water, elemental impurities, and UV background for use in quantitative analytics.
Stabilizers/salts: If the compound is supplied as a salt or with stabilizers, this affects molecular weight for dosing calculations and may influence solubility and storage. Always check the form stated in the CoA.
Recommended quality controls (user practice)
Verify identity by orthogonal methods where critical (HPLC-UV/ELSD, HRMS). For sensitive applications, consider qNMR for assay.
Document water content (Karl Fischer) and residual solvent profile if your protocol is moisture-sensitive or requires stringent background control.
For screening campaigns, standardize on a defined purity cutoff (often ≥95% by area, UV 210–254 nm) and track lot-to-lot variability.
Reaction and Applications
Applicability to chemical synthesis (honest scope)
AZ82 is supplied as a research compound for screening/biological studies. It is not typically employed as a reagent, catalyst, monomer, or solvent in preparative organic synthesis. Consequently, there are no standard “synthetic reaction” applications to document for this item.
Research applications (general, literature context; no medical claims)
Small-molecule tool compounds like AZ82 are commonly used in target validation, pathway interrogation, and phenotypic screening in cell-based or biochemical assays. Typical workflows include concentration–response profiling, mechanism-of-action studies in vitro, and orthogonal confirmation assays.
Data reliability improves with parallel use of structural analogs, inactive controls, and counterscreens to exclude off-target or assay-interference effects.
Practical guidance
Prepare a well-characterized DMSO stock (commonly 10 mM), confirm solution clarity, and aliquot to avoid freeze–thaw cycles.
Establish assay windows with positive/negative controls and verify time-dependence and reversibility where mechanistic insights are sought.
Note
For any claim of biological activity or selectivity of “AZ82,” consult the primary literature and verify that the CAS 1449578-65-7/CID 91885438 corresponds to the specific compound and mechanism you intend to study.
Reaction Conditions
Not typically applicable
AZ82 is not used to mediate chemical transformations; therefore, there are no standard reaction conditions (solvent, temperature, catalysts) to report for this item.
Related practical considerations (general)
For assay work, establish dosing conditions rather than reaction conditions: solvent system (commonly DMSO stocks), incubation time, temperature (often 25–37°C), and exposure duration appropriate to the biological system. Validate compound stability over the assay time course by re-analyzing working solutions when feasible.
Safety and Handling
Item-specific hazard data (from Product Data)
Signal word: Not specified for this item; refer to SDS.
H-statements: Not specified for this item; refer to SDS.
GHS classification/pictograms: Not specified for this item; refer to SDS.
General laboratory precautions (professional guidance; defer to SDS)
Handle as a bioactive research chemical of unknown acute toxicity: work in a chemical fume hood with appropriate PPE (lab coat, safety glasses, nitrile gloves). Avoid inhalation of dust/aerosols and skin contact.
Prevent exposure to light/air/moisture if the CoA indicates sensitivity; keep containers tightly closed. Use single-use, pre-chilled aliquots to avoid repeated warming for materials stored at −80°C.
Incompatibilities: In absence of specific data, segregate from strong oxidizers and acids/bases until stability is known. Avoid contact with reactive reducing/oxidizing agents.
First-aid overview (consult SDS for definitive instructions)
Skin/eye contact: Rinse with water for ≥15 minutes; remove contaminated clothing; seek medical attention.
Inhalation: Move to fresh air; provide oxygen if needed; seek medical attention.
Ingestion: Rinse mouth; do not induce vomiting; seek medical attention.
Spill and waste
Small spills: Absorb with inert material (e.g., vermiculite), collect in a sealable container for hazardous waste.
Disposal: Treat as hazardous organic chemical waste following institutional and regulatory guidelines.
Solvent Selection
Item-specific
No solubility profile provided for this item; refer to CoA/Spec Sheet.
General guidance for bioactive small molecules
Primary stock solvent: Anhydrous DMSO is typically preferred due to broad solvating power and compatibility with biochemical and cell-based assays.
Working solutions: Dilute DMSO stocks into assay buffers or culture media immediately before use; maintain final DMSO at ≤0.1–0.5% v/v unless your system tolerates higher levels. Include vehicle controls matched to the highest DMSO percentage.
Alternative cosolvents: DMF or ethanol (absolute) may aid solubilization for hydrophobic chemotypes. For aqueous delivery, consider adding nonionic surfactants at low levels (e.g., 0.01–0.05% Tween-20) where biologically acceptable.
Practical tips
Warm gently (ambient to 37°C) and vortex/sonicate to dissolve; avoid prolonged heating. Filter sterilize (0.22 µm) only if compatible with solvent and solute.
For kinetic/biophysical assays, pre-equilibrate solutions to the assay temperature and verify absence of precipitate by light scattering or visual inspection.
Comparison (general)
DMSO vs ethanol: DMSO offers higher solubility for diverse scaffolds; ethanol provides faster evaporation but narrower solubility scope. Choose based on assay tolerance and required stock concentration.
Storage and Reconstitution
Item-specific (from Product Data)
Storage conditions: Store at −80°C.
Shipping: Shipped on dry ice packs + cold packs.
General storage guidance for bioactive small molecules
Keep tightly sealed in an inert, dry atmosphere. If hygroscopic or air/light-sensitive (check CoA), handle under low humidity, protect from light, and purge headspace with inert gas after each opening.
Long-term storage: −80°C is appropriate for maximal stability; avoid frost-free freezers. Use small, single-use aliquots to prevent repeated thawing.
Reconstitution (general recommendations; verify with CoA)
Solvent: Dissolve in anhydrous DMSO to prepare a concentrated stock (commonly 10–50 mM). Mix by gentle vortexing and, if needed, brief sonication. Do not heat above 40°C.
Filtration: If sterility is required and the compound/solvent are compatible, filter through a 0.22 µm PTFE or PES membrane. Avoid cellulose esters with strong aprotic solvents.
Working solutions: Dilute into assay buffer or culture medium immediately before use; maintain constant final DMSO across all treatments and controls.
Stability and documentation
Record preparation date, concentration, and lot number on each aliquot. Periodically re-check concentration and purity by HPLC/LC–MS during extended studies.
Research use note
For research use only. Not for human or veterinary use.
Structure and Identity
Item-specific (from Product Data)
SKU: A655482
Product name: AZ82
CAS: 1449578-65-7
PubChem CID: 91885438
InChIKey: Not specified for this item; refer to CoA/Spec Sheet.
SMILES: Not specified for this item; refer to CoA/Spec Sheet.
Molecular formula: Not specified for this item; refer to CoA/Spec Sheet.
Molecular weight: Not specified for this item; refer to CoA/Spec Sheet.
Structural features (general/literature context, no item-specific claims)
AZ82 is referenced in the literature as a small-molecule research tool compound; specific structural motifs, ring systems, and stereochemistry should be confirmed against primary references or the CoA.
Without a provided structure, do not infer functional groups or atom connectivity. Use the CAS and CID above to retrieve authoritative identifiers if needed in your laboratory’s documentation system.
2D structure description (general guidance)
Not described here due to missing SMILES/InChI. Obtain a verified structure drawing from a trusted database (e.g., supplier CoA, PubChem entry for CID 91885438) before performing cheminformatics, inventory registration, or computational modeling.
Synthetic Utility
Applicability to synthesis
AZ82 is a finalized small-molecule entity meant for biological evaluation rather than as a building block. It does not have documented use as a reagent, protecting group, coupling partner, or ligand in standard synthetic methodologies.
General guidance
If structural analogs of AZ82 are to be prepared for SAR, plan retrosynthesis around the confirmed core scaffold obtained from literature/CoA. Typical workflows include modular introduction of substituents, late-stage diversification, and parallel synthesis to explore potency/ADME space.
Analytical control (HPLC, HRMS, NMR) and impurity profiling are critical to ensure that observed biological effects are attributable to the intended structure rather than byproducts or residual reagents.
Target Specificity
Item-specific
No target, selectivity profile, or binding data are provided in the Product Data for AZ82.
Guidance
If your study requires target-specific conclusions, consult peer-reviewed literature for compounds identified as “AZ82” under CAS 1449578-65-7 / CID 91885438, and confirm alignment with your lot’s identity. Consider profiling against related target families to quantify selectivity and off-target liabilities.
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