AZD1208 - Moligand™, 10mM in DMSO , Inhibitor of Pim-1 proto-oncogene; serine/threonine kinase;Inhibitor of Pim-2 proto-oncogene; serine/threonine kinase;Inhibitor of Pim-3 proto-oncogene; serine/threonine kinase, CAS No.1204144-28-4, Inhibitor of Pim-1 proto-oncogene; serine/threonine kinase;Inhibitor of Pim-2 proto-oncogene; serine/threonine kinase;Inhibitor of Pim-3 proto-oncogene; serine/threonine kinase

CAS: 1204144-28-4 Cat. No.: A420898 Fórmula: C₂₁H₂₁N₃O₂S Peso molecular: 379.48

GRADE & PURITY Moligand™, 10mM in DMSO

Synonyms

AZD 1208 [WHO-DD] | Q27074698 | FT-0633869 | J-013789 | HY-15604 | S98NFM1378 | SW219766-1 | 1204144-28-4 | AZ-1208 | (R,Z)-5-((2-(3-Aminopiperidin-1-yl)biphenyl-3-yl)methylene)thiazolidine-2,4-dione | AZD1208 | AZD-1208 | NSC776066 | NSC-776066 | (R,Z)-5

Storage

Store at -80°C

Shipped In

Dry ice packs + Cold packs

Visão geral

AZD1208 is a potent, and orally available Pim kinase inhibitor with IC50 of 0.4 nM, 5 nM, and 1.9 nM for Pim1, Pim2, and Pim3 in cell-free assays, respectively.

Specifications

Sinónimos

AZD 1208 [WHO-DD] | Q27074698 | FT-0633869 | J-013789 | HY-15604 | S98NFM1378 | SW219766-1 | 1204144-28-4 | AZ-1208 | (R,Z)-5-((2-(3-Aminopiperidin-1-yl)biphenyl-3-yl)methylene)thiazolidine-2,4-dione | AZD1208 | AZD-1208 | NSC776066 | NSC-776066 | (R,Z)-5

Especificações e pureza

Moligand™, 10mM in DMSO

Mecanismos bioquímicos e fisiológicos

Potent pan Pim kinase inhibitor (IC50values are 0.4, 1.9 and 5.0 nM for Pim-1, 3 and 2, respectively). Exhibits >43-fold higher affinity for Pim kinases over a range of other kinases. Induces cell cycle arrest and apoptosis in AML cell linesin vitroand in

Condições de armazenamento de armazenamento

Store at -80°C

Enviado em

Dry ice packs + Cold packs

Este produto requer transporte de cadeia fria. Serviços terrestres e outros serviços econômicos não estão disponíveis.

Grau

Moligand™

Tipo de ação

INHIBITOR

Mecanismo de ação

Inhibitor of Pim-1 proto-oncogene; serine/threonine kinase;Inhibitor of Pim-2 proto-oncogene; serine/threonine kinase;Inhibitor of Pim-3 proto-oncogene; serine/threonine kinase

Nomes e identificadores

• Sorrisos canónicos: C1CC(CN(C1)C2=C(C=CC=C2C3=CC=CC=C3)C=C4C(=O)NC(=O)S4)N
• IUPAC Name: (5Z)-5-[[2-[(3R)-3-aminopiperidin-1-yl]-3-phenylphenyl]methylidene]-1,3-thiazolidine-2,4-dione
• InChIKey: MCUJKPPARUPFJM-UWCCDQBKSA-N
• INCHI: 1S/C21H21N3O2S/c22-16-9-5-11-24(13-16)19-15(12-18-20(25)23-21(26)27-18)8-4-10-17(19)14-6-2-1-3-7-14/h1-4,6-8,10,12,16H,5,9,11,13,22H2,(H,23,25,26)/b18-12-/t16-/m1/s1
• SMILES isoméricas: C1C[C@H](CN(C1)C2=C(C=CC=C2C3=CC=CC=C3)/C=C\4/C(=O)NC(=O)S4)N
• Peso molecular: 379.48
• Reaxy-Rn: 30682637
• Reaxys-RN_link_address: https://www.reaxys.com/reaxys/secured/hopinto.do?context=S&query=IDE.XRN=30682637&ln=

Taxonomic Classification

Taxonomy Tree

• Kingdom Organic compounds
  • Superclass Organoheterocyclic compounds
    • Classe Piperidines
      • Subclass Phenylpiperidines

• Kingdom: Organic compounds
• Superclass: Organoheterocyclic compounds
• Classe: Piperidines
• Subclass: Phenylpiperidines
• Intermediate Tree Nodes: Not available
• Direct Parent: Phenylpiperidines
• Alternative Parents: Biphenyls and derivatives Dialkylarylamines Aniline and substituted anilines Thiazolidinediones Aminopiperidines Dicarboximides Thiocarbamic acid derivatives Amino acids and derivatives Azacyclic compounds Organic oxides Monoalkylamines Hydrocarbon derivatives Carbonyl compounds
• Molecular Framework: Aromatic heteromonocyclic compounds
• Substituents: Biphenyl - Phenylpiperidine - Aniline or substituted anilines - Dialkylarylamine - Tertiary aliphatic/aromatic amine - 3-aminopiperidine - Thiazolidinedione - Monocyclic benzene moiety - Benzenoid - Thiazolidine - Dicarboximide - Amino acid or derivatives - Thiocarbamic acid derivative - Tertiary amine - Azacycle - Carboxylic acid derivative - Amine - Organonitrogen compound - Primary aliphatic amine - Organooxygen compound - Primary amine - Carbonyl group - Hydrocarbon derivative - Organic oxide - Organic oxygen compound - Organic nitrogen compound - Aromatic heteromonocyclic compound
• Descrição: This compound belongs to the class of organic compounds known as phenylpiperidines. These are compounds containing a phenylpiperidine skeleton, which consists of a piperidine bound to a phenyl group.
• External Descriptors: Not available

Data sources

1. Djoumbou Feunang Y, Eisner R, Knox C, Chepelev L, Hastings J, Owen G, Fahy E, Steinbeck C, Subramanian S, Bolton E, Greiner R, and Wishart DS. ClassyFire: Automated Chemical Classification With A Comprehensive, Computable Taxonomy. Journal of Cheminformatics, 2016, 8:61.

DOI: 10.1186/s13321-016-0174-y

Alvos associados (humanos)

PIM1 Tchem Serine/threonine-protein kinase pim-1 (7 Activities)

Discover Target: PIM1

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

PIM2 Tchem Serine/threonine-protein kinase pim-2 (5 Activities)

Discover Target: PIM2

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

PIM3 Tchem Serine/threonine-protein kinase pim-3 (6 Activities)

Discover Target: PIM3

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

ABL1 Tclin Tyrosine-protein kinase ABL (18331 Activities)

Discover Target: ABL1

Activity Type	Relation	Activity value	Units	Action Type	Journal	PubMed Id	doi	Assay Aladdin ID

EGFR Tclin Epidermal growth factor receptor erbB1 (33727 Activities)

Discover Target: EGFR

Activity Type	Relation	Activity value	Units	Action Type	Journal	PubMed Id	doi	Assay Aladdin ID

INSR Tclin Insulin receptor (5558 Activities)

Discover Target: INSR

Activity Type	Relation	Activity value	Units	Action Type	Journal	PubMed Id	doi	Assay Aladdin ID

TOP2A Tclin DNA topoisomerase II alpha (6317 Activities)

Discover Target: TOP2A

Activity Type	Relation	Activity value	Units	Action Type	Journal	PubMed Id	doi	Assay Aladdin ID

IMPDH2 Tclin Inosine-5'-monophosphate dehydrogenase 2 (1326 Activities)

Discover Target: IMPDH2

Activity Type	Relation	Activity value	Units	Action Type	Journal	PubMed Id	doi	Assay Aladdin ID

KCNH2 Tclin HERG (29587 Activities)

Discover Target: KCNH2

Activity Type	Relation	Activity value	Units	Action Type	Journal	PubMed Id	doi	Assay Aladdin ID

PDGFRB Tclin Platelet-derived growth factor receptor beta (5195 Activities)

Discover Target: PDGFRB

Activity Type	Relation	Activity value	Units	Action Type	Journal	PubMed Id	doi	Assay Aladdin ID

IGF1R Tclin Insulin-like growth factor I receptor (8605 Activities)

Discover Target: IGF1R

Activity Type	Relation	Activity value	Units	Action Type	Journal	PubMed Id	doi	Assay Aladdin ID

DCK Tchem Deoxycytidine kinase (530 Activities)

Discover Target: DCK

Activity Type	Relation	Activity value	Units	Action Type	Journal	PubMed Id	doi	Assay Aladdin ID

MAPK11 Tchem MAP kinase p38 beta (2785 Activities)

Discover Target: MAPK11

Activity Type	Relation	Activity value	Units	Action Type	Journal	PubMed Id	doi	Assay Aladdin ID

LCK Tclin Tyrosine-protein kinase LCK (9212 Activities)

Discover Target: LCK

Activity Type	Relation	Activity value	Units	Action Type	Journal	PubMed Id	doi	Assay Aladdin ID

NQO2 Tchem Quinone reductase 2 (885 Activities)

Discover Target: NQO2

Activity Type	Relation	Activity value	Units	Action Type	Journal	PubMed Id	doi	Assay Aladdin ID

ADK Tchem Adenosine kinase (1481 Activities)

Discover Target: ADK

Activity Type	Relation	Activity value	Units	Action Type	Journal	PubMed Id	doi	Assay Aladdin ID

MAPK14 Tchem MAP kinase p38 alpha (12866 Activities)

Discover Target: MAPK14

Activity Type	Relation	Activity value	Units	Action Type	Journal	PubMed Id	doi	Assay Aladdin ID

AK2 Tbio Adenylate kinase 2 (264 Activities)

Discover Target: AK2

Activity Type	Relation	Activity value	Units	Action Type	Journal	PubMed Id	doi	Assay Aladdin ID

GSK3B Tclin Glycogen synthase kinase-3 beta (11785 Activities)

Discover Target: GSK3B

Activity Type	Relation	Activity value	Units	Action Type	Journal	PubMed Id	doi	Assay Aladdin ID

PRKACB Tchem cAMP-dependent protein kinase beta-1 catalytic subunit (589 Activities)

Discover Target: PRKACB

Activity Type	Relation	Activity value	Units	Action Type	Journal	PubMed Id	doi	Assay Aladdin ID

MAP2K6 Tchem Dual specificity mitogen-activated protein kinase kinase 6 (1284 Activities)

Discover Target: MAP2K6

Activity Type	Relation	Activity value	Units	Action Type	Journal	PubMed Id	doi	Assay Aladdin ID

MYLK Tchem Myosin light chain kinase, smooth muscle (1267 Activities)

Discover Target: MYLK

Activity Type	Relation	Activity value	Units	Action Type	Journal	PubMed Id	doi	Assay Aladdin ID

CSNK1G1 Tchem Casein kinase I gamma 1 (2496 Activities)

Discover Target: CSNK1G1

Activity Type	Relation	Activity value	Units	Action Type	Journal	PubMed Id	doi	Assay Aladdin ID

FGFR1 Tclin Fibroblast growth factor receptor 1 (9149 Activities)

Discover Target: FGFR1

Activity Type	Relation	Activity value	Units	Action Type	Journal	PubMed Id	doi	Assay Aladdin ID

STK3 Tchem Serine/threonine-protein kinase MST2 (3069 Activities)

Discover Target: STK3

Activity Type	Relation	Activity value	Units	Action Type	Journal	PubMed Id	doi	Assay Aladdin ID

PRKAG1 Tchem AMP-activated protein kinase, gamma-1 subunit (254 Activities)

Discover Target: PRKAG1

Activity Type	Relation	Activity value	Units	Action Type	Journal	PubMed Id	doi	Assay Aladdin ID

CSNK1D Tchem Casein kinase I delta (4546 Activities)

Discover Target: CSNK1D

Activity Type	Relation	Activity value	Units	Action Type	Journal	PubMed Id	doi	Assay Aladdin ID

MAP2K5 Tchem Dual specificity mitogen-activated protein kinase kinase 5 (650 Activities)

Discover Target: MAP2K5

Activity Type	Relation	Activity value	Units	Action Type	Journal	PubMed Id	doi	Assay Aladdin ID

SRC Tclin Tyrosine-protein kinase SRC (10310 Activities)

Discover Target: SRC

Activity Type	Relation	Activity value	Units	Action Type	Journal	PubMed Id	doi	Assay Aladdin ID

MAP2K3 Tchem Dual specificity mitogen-activated protein kinase kinase 3 (632 Activities)

Discover Target: MAP2K3

Activity Type	Relation	Activity value	Units	Action Type	Journal	PubMed Id	doi	Assay Aladdin ID

PRKAG2 Tchem AMP-activated protein kinase, gamma-2 subunit (243 Activities)

Discover Target: PRKAG2

Activity Type	Relation	Activity value	Units	Action Type	Journal	PubMed Id	doi	Assay Aladdin ID

AKT2 Tchem Serine/threonine-protein kinase AKT2 (4301 Activities)

Discover Target: AKT2

Activity Type	Relation	Activity value	Units	Action Type	Journal	PubMed Id	doi	Assay Aladdin ID

AKT3 Tchem Serine/threonine-protein kinase AKT3 (3157 Activities)

Discover Target: AKT3

Activity Type	Relation	Activity value	Units	Action Type	Journal	PubMed Id	doi	Assay Aladdin ID

GRK2 Tchem G-protein coupled receptor kinase 2 (1019 Activities)

Discover Target: GRK2

Activity Type	Relation	Activity value	Units	Action Type	Journal	PubMed Id	doi	Assay Aladdin ID

PYGB Tchem Brain glycogen phosphorylase (474 Activities)

Discover Target: PYGB

Activity Type	Relation	Activity value	Units	Action Type	Journal	PubMed Id	doi	Assay Aladdin ID

AURKB Tchem Serine/threonine-protein kinase Aurora-B (6805 Activities)

Discover Target: AURKB

Activity Type	Relation	Activity value	Units	Action Type	Journal	PubMed Id	doi	Assay Aladdin ID

RPS6KA4 Tchem Ribosomal protein S6 kinase alpha 4 (2104 Activities)

Discover Target: RPS6KA4

Activity Type	Relation	Activity value	Units	Action Type	Journal	PubMed Id	doi	Assay Aladdin ID

ROCK1 Tclin Rho-associated protein kinase 1 (4723 Activities)

Discover Target: ROCK1

Activity Type	Relation	Activity value	Units	Action Type	Journal	PubMed Id	doi	Assay Aladdin ID

PIM1 Tchem Serine/threonine-protein kinase PIM1 (9629 Activities)

Discover Target: PIM1

Activity Type	Relation	Activity value	Units	Action Type	Journal	PubMed Id	doi	Assay Aladdin ID

PKN2 Tchem Protein kinase N2 (1991 Activities)

Discover Target: PKN2

Activity Type	Relation	Activity value	Units	Action Type	Journal	PubMed Id	doi	Assay Aladdin ID

CSK Tchem Tyrosine-protein kinase CSK (2395 Activities)

Discover Target: CSK

Activity Type	Relation	Activity value	Units	Action Type	Journal	PubMed Id	doi	Assay Aladdin ID

RPS6KA5 Tchem Ribosomal protein S6 kinase alpha 5 (3355 Activities)

Discover Target: RPS6KA5

Activity Type	Relation	Activity value	Units	Action Type	Journal	PubMed Id	doi	Assay Aladdin ID

PRKD3 Tchem Protein kinase C nu (2315 Activities)

Discover Target: PRKD3

Activity Type	Relation	Activity value	Units	Action Type	Journal	PubMed Id	doi	Assay Aladdin ID

PRKCI Tchem Protein kinase C iota (2821 Activities)

Discover Target: PRKCI

Activity Type	Relation	Activity value	Units	Action Type	Journal	PubMed Id	doi	Assay Aladdin ID

SYK Tclin Tyrosine-protein kinase SYK (7372 Activities)

Discover Target: SYK

Activity Type	Relation	Activity value	Units	Action Type	Journal	PubMed Id	doi	Assay Aladdin ID

PLK1 Tchem Serine/threonine-protein kinase PLK1 (28605 Activities)

Discover Target: PLK1

Activity Type	Relation	Activity value	Units	Action Type	Journal	PubMed Id	doi	Assay Aladdin ID

JAK1 Tclin Tyrosine-protein kinase JAK1 (8569 Activities)

Discover Target: JAK1

Activity Type	Relation	Activity value	Units	Action Type	Journal	PubMed Id	doi	Assay Aladdin ID

RPS6KA1 Tchem Ribosomal protein S6 kinase alpha 1 (2796 Activities)

Discover Target: RPS6KA1

Activity Type	Relation	Activity value	Units	Action Type	Journal	PubMed Id	doi	Assay Aladdin ID

RPS6KA6 Tchem Ribosomal protein S6 kinase alpha 6 (2027 Activities)

Discover Target: RPS6KA6

Activity Type	Relation	Activity value	Units	Action Type	Journal	PubMed Id	doi	Assay Aladdin ID

PFKP Tbio Phosphofructokinase platelet type (224 Activities)

Discover Target: PFKP

Activity Type	Relation	Activity value	Units	Action Type	Journal	PubMed Id	doi	Assay Aladdin ID

CSNK1E Tclin Casein kinase I epsilon (1412 Activities)

Discover Target: CSNK1E

Activity Type	Relation	Activity value	Units	Action Type	Journal	PubMed Id	doi	Assay Aladdin ID

GSK3A Tclin Glycogen synthase kinase-3 alpha (3764 Activities)

Discover Target: GSK3A

Activity Type	Relation	Activity value	Units	Action Type	Journal	PubMed Id	doi	Assay Aladdin ID

PRKG1 Tchem cGMP-dependent protein kinase 1 beta (2814 Activities)

Discover Target: PRKG1

Activity Type	Relation	Activity value	Units	Action Type	Journal	PubMed Id	doi	Assay Aladdin ID

Alvos associados (não humanos)

Cdk4 Cyclin-dependent kinase 4 (3 Activities)

Discover Target: Cdk4

Activity Type	Relation	Activity value	Units	Action Type	Journal	PubMed Id	doi	Assay Aladdin ID

Pygl Liver glycogen phosphorylase (81 Activities)

Discover Target: Pygl

Activity Type	Relation	Activity value	Units	Action Type	Journal	PubMed Id	doi	Assay Aladdin ID

Prkcd Protein kinase C delta (192 Activities)

Discover Target: Prkcd

Activity Type	Relation	Activity value	Units	Action Type	Journal	PubMed Id	doi	Assay Aladdin ID

Prkcb Protein kinase C beta (2 Activities)

Discover Target: Prkcb

Activity Type	Relation	Activity value	Units	Action Type	Journal	PubMed Id	doi	Assay Aladdin ID

Mapk1 MAP kinase ERK2 (650 Activities)

Discover Target: Mapk1

Activity Type	Relation	Activity value	Units	Action Type	Journal	PubMed Id	doi	Assay Aladdin ID

Hdac6 Histone deacetylase 6 (222 Activities)

Discover Target: Hdac6

Activity Type	Relation	Activity value	Units	Action Type	Journal	PubMed Id	doi	Assay Aladdin ID

Fgfr3 Fibroblast growth factor receptor 3 (21 Activities)

Discover Target: Fgfr3

Activity Type	Relation	Activity value	Units	Action Type	Journal	PubMed Id	doi	Assay Aladdin ID

Camk2g CaM kinase II gamma (1 Activities)

Discover Target: Camk2g

Activity Type	Relation	Activity value	Units	Action Type	Journal	PubMed Id	doi	Assay Aladdin ID

Tec Tyrosine-protein kinase TEC (25 Activities)

Discover Target: Tec

Activity Type	Relation	Activity value	Units	Action Type	Journal	PubMed Id	doi	Assay Aladdin ID

AIRC Multifunctional protein ADE2 (8 Activities)

Discover Target: AIRC

Activity Type	Relation	Activity value	Units	Action Type	Journal	PubMed Id	doi	Assay Aladdin ID

rep Replicase polyprotein 1ab (378 Activities)

Discover Target: rep

Activity Type	Relation	Activity value	Units	Action Type	Journal	PubMed Id	doi	Assay Aladdin ID

Septin9 Septin-9 (1 Activities)

Discover Target: Septin9

Activity Type	Relation	Activity value	Units	Action Type	Journal	PubMed Id	doi	Assay Aladdin ID

Acadvl Very long-chain specific acyl-CoA dehydrogenase, mitochondrial (1 Activities)

Discover Target: Acadvl

Activity Type	Relation	Activity value	Units	Action Type	Journal	PubMed Id	doi	Assay Aladdin ID

Mecanismos de ação

Propriedades químicas e físicas

• Peso molecular: 379.500 g/mol
• XLogP3: 3.600
• Hydrogen Bond Donor Count: 2
• Hydrogen Bond Acceptor Count: 5
• Rotatable Bond Count: 3
• Exact Mass: 379.135 Da
• Monoisotopic Mass: 379.135 Da
• Topological Polar Surface Area: 101.000 Ų
• Heavy Atom Count: 27
• Formal Charge: 0
• Complexity: 602.000
• Isotope Atom Count: 0
• Defined Atom Stereocenter Count: 1
• Undefined Atom Stereocenter Count: 0
• Defined Bond Stereocenter Count: 1
• Undefined Bond Stereocenter Count: 0
• The total count of all stereochemical bonds: 1
• Covalently-Bonded Unit Count: 1

Application Protocols

Only information from Product Data may be listed here.

• Tested applications (e.g., WB, IHC, IF, FC) and recommended dilutions: Not specified for this item; refer to CoA/Spec Sheet.

General guidance for use (non-validated, for planning only):

• For biochemical assays, prepare serial dilutions from DMSO stocks; include vehicle controls; consider triplicate wells per concentration.
• For cell-based assays, confirm solubility and absence of precipitation upon dilution into media; maintain consistent final DMSO.

Note: Follow your lab’s validated protocols. This product is supplied without application-specific validation data in the Product Data.

Biological Roles

Context (literature/probe information; no clinical claims):

• AZD1208 is widely cited as a pan-PIM kinase inhibitor (PIM1, PIM2, PIM3) used to interrogate the roles of these constitutively active serine/threonine kinases in cellular signaling. PIM kinases are implicated in regulation of cell cycle, apoptosis pathways (e.g., BAD phosphorylation), and metabolism.
• As a chemical probe, AZD1208 is applied to dissect the contribution of PIM signaling to transcriptional regulation, protein translation (e.g., via 4E-BP1 pathways), and survival signaling. It enables cause–effect studies by pharmacological perturbation alongside genetic methods (RNAi/CRISPR).
• In biochemical systems, AZD1208 typically competes at the ATP-binding site of PIM kinases, allowing potency and selectivity mapping across the kinome in standardized panels (literature use).

Practical use in discovery biology:

• Dose–response studies in enzymatic or cellular assays to establish potency (IC50/EC50) and functional consequences on downstream phosphorylation events (quantified by phospho-specific antibodies or MS-based phosphoproteomics).
• Combination perturbations with synergistic pathways (e.g., PI3K/AKT/mTOR) are frequently explored in research to map network dependencies; ensure proper controls to attribute effects correctly.

Note: Exact potency values, selectivity matrices, and assay outcomes are assay-dependent and should be determined under your conditions or sourced from peer-reviewed literature.

Buffer Applications

AZD1208 is not a buffering agent. It does not define buffer capacity or pH ranges. For biological assays, it is typically diluted from DMSO stocks into standard assay buffers (e.g., HEPES-, Tris-, or phosphate-based).

Practical notes:

• Maintain consistent final DMSO content across treated and control wells to avoid confounding effects on enzyme or cell behavior.
• Use buffers compatible with your detection modality (e.g., low autofluorescence for fluorescence readouts; volatile components for LC–MS sample prep).
• If adsorption or precipitation is observed at low concentrations, add minimal non-ionic surfactant (e.g., 0.01% Tween-20) when compatible with the assay, or increase protein carrier (e.g., BSA) as appropriate.

Specifications such as pKa, isoelectric point, or buffering range are not applicable to this small-molecule inhibitor.

Green Alternatives

Because AZD1208 is a fixed structure small-molecule tool compound, “green alternatives” pertain mainly to solvent and process choices rather than replacing the compound itself.

Greener handling strategies (general):

• Prefer water-miscible solvents with safer profiles where feasible. DMSO is broadly accepted in screening for its comparatively favorable safety profile versus DMF/NMP.
• For chromatography, use water–acetonitrile with volatile buffers instead of halogenated solvents. Optimize gradients to reduce solvent consumption.
• Minimize DMSO usage by preparing higher-concentration stocks when solubility permits and dispensing with acoustic or low-volume pipetting.
• Implement microplate formats and miniaturized assays to reduce reagent and solvent waste.

Comparison (general solvent considerations):

• DMSO vs DMF/NMP: DMSO offers lower volatility and a more favorable EHS profile; DMF/NMP may increase solubility for some hydrophobic compounds but carry stricter hazard classifications.
• Acetonitrile vs methanol for LC: acetonitrile typically provides lower backpressure and improved peak shape; methanol is less toxic but can reduce MS sensitivity for some analytes.

Note: No alternative “green” compound can substitute AZD1208’s specific target engagement profile; sustainability gains should focus on solvent selection, scale minimization, and proper waste management.

Pharmaceutical Uses

This product is supplied strictly for research use only and is not manufactured under GMP. No clinical or therapeutic claims are made.

Relevant research/formulation contexts (general):

• Reference standard in method development and validation for discovery-stage bioanalytical assays (e.g., LC–MS quantitation in in vitro ADME systems).
• Tool compound for preclinical discovery to evaluate target engagement, permeability, and stability in model systems.
• Potential use in formulation screening at micro-scale to evaluate solubilization strategies (co-solvents, pH modifiers, amorphous dispersions) for in vitro testing; results are method-development data only.

Regulatory notes:

• Not compendial (no pharmacopeial monograph known for AZD1208); not suitable for human use.
• For any regulated studies, obtain materials under the appropriate quality system and with complete characterization packages beyond the scope of this Moligand™ listing.

Item-specific excipient or formulation data: Not specified for this item; refer to CoA/Spec Sheet.

Physical Properties

Item-specific specifications are not provided in the Product Data for this listing. For exact values (e.g., mp, solubility, water content), consult the CoA/Spec Sheet.

• Appearance: Not specified for this item; refer to CoA/Spec Sheet.
• Molecular weight: Not specified for this item; refer to CoA/Spec Sheet.
• Molecular formula: Not specified for this item; refer to CoA/Spec Sheet.
• Melting point: Not specified for this item; refer to CoA/Spec Sheet. (Literature values for AZD1208 vary by polymorph and supplier; verify experimentally.)
• Solubility (practical guidance, literature/general):
  • Often formulated for screening as DMSO stock solutions (e.g., 10–50 mM) due to limited aqueous solubility typical of kinase inhibitors.
  • Aqueous solubility may be enhanced with co-solvents (DMSO, DMF), surfactants, or pH adjustment if ionizable groups are present (verify with CoA/SDS before use).
• LogP/logD, pKa, refractive index, density: Not specified for this item; refer to CoA/Spec Sheet. (Use literature or computational tools for preliminary estimates, then confirm experimentally.)

Practical notes (general):

• For HTS use, prepare concentrated DMSO stocks and dilute into assay buffer to minimal final DMSO (e.g., ≤0.5–1% v/v) to limit vehicle effects.
• Filter sterilization (0.22 μm PTFE) may be used for biological assays if compatible with the solvent system.

Quality and Grades

• Grade/Purity: Moligand™

About Moligand™ (general description of intent):

• Moligand™ products are curated for use as small-molecule tool compounds in screening, target-validation, and medicinal chemistry workflows. While not equivalent to GMP or clinical grades, Moligand™ emphasizes identity confirmation and suitability for discovery research.

What this means for AZD1208 (this item):

• Intended for research and screening applications where consistent identity and convenient formulation are important.
• UV cutoffs, water/peroxide/metal impurities, and stabilizers: Not specified for this item; refer to CoA/Spec Sheet.
• Chromatographic suitability (e.g., HPLC/LC–MS reference use): feasible as a research standard; confirm purity and counterion/solvate state on the CoA before quantitative work.

Documentation and testing (expectations):

• Identity confirmation typically by NMR/LC–MS/HRMS where applicable (verify on CoA).
• Purity reporting (area % by HPLC) and residual solvent content may be provided on the CoA; verify acceptance criteria align with your assay sensitivity.

When to choose Moligand™:

• Early discovery assays (biochemical/biophysical), cell-based target engagement, orthogonal confirmation studies, or as a comparator in SAR series. For regulated uses or clinical development, source under appropriate quality systems (not supplied here).

Reaction and Applications

This product is provided as a research-grade small-molecule tool compound rather than a synthetic reagent.

Applications (research use, literature/general):

• Widely utilized as a chemical probe for PIM kinases (PIM1/2/3) in biochemical assays, biophysical binding studies (e.g., thermal shift/DSF, SPR), and cell-based target engagement experiments.
• Employed in kinase selectivity profiling panels to benchmark off-target interactions and assess structure–activity relationships relative to other PIM inhibitors.
• Useful as a reference standard in LC–MS and HPLC method development for quantifying exposure, stability, or permeability in discovery workflows.

Practical considerations:

• Prepare DMSO stocks (light-protect as appropriate) and aliquot to avoid multiple freeze–thaw cycles.
• Include vehicle controls matched to the final DMSO percentage in assays.
• For enzyme assays, preincubation with protein may affect observed potency depending on binding kinetics; standardize preincubation times and temperature.
• Adsorptive losses can occur at low nM concentrations; minimize plastic contact or use low-binding labware.

Not typically applicable:

• This compound is not generally used as a reagent in named organic reactions; it is better treated as a bioactive small molecule for screening and analytical purposes.

Reaction Conditions

Not typically applicable. AZD1208 is provided as a research tool compound rather than a substrate/reagent for carrying out chemical reactions.

Assay-related conditions (general research practice):

• Stock preparation: dissolve in DMSO (e.g., 10–50 mM), vortex/sonicate if needed, and sterile-filter if required by the assay.
• Storage/handling: keep stocks at low temperature and protected from light/moisture as appropriate; minimize freeze–thaw cycles by aliquoting.
• Biochemical assays: incubate with target kinase under standard assay buffer conditions (e.g., HEPES/Tris, Mg2+, ATP at Km) and measure inhibition via ADP-coupled or radiometric readouts. Optimize preincubation time if slow-binding kinetics are suspected.
• Cellular assays: titrate to define concentration–response; monitor cytotoxicity of vehicle and compound; maintain consistent solvent percentages across conditions.

Reminder: Exact reaction or assay parameters (temperature, time, cofactors) are system- and method-specific and should be optimized empirically.

Safety and Handling

• GHS classification, signal word, pictograms, and H-statements: Not specified for this item; refer to SDS.
• Research Use: For research use only. Not for human or veterinary use.

General laboratory safety guidance (defer to SDS as authoritative):

• PPE: laboratory coat, safety glasses, and appropriate chemical-resistant gloves. Handle powders/solids in a fume hood to avoid inhalation.
• Avoid ingestion, inhalation, and skin/eye contact. Wash thoroughly after handling. Prevent dust/aerosol formation during weighing.
• Storage incompatibilities (general): keep away from strong oxidizers and strong acids/bases unless compatibility is known. Avoid prolonged exposure to light and moisture if the compound is known or suspected to be photosensitive/hygroscopic (check CoA/SDS).
• First aid (general):
  • Inhalation: move to fresh air; seek medical attention if symptoms persist.
  • Skin: wash with soap and water; remove contaminated clothing.
  • Eyes: rinse cautiously with water for several minutes; remove contact lenses if present and easy to do; seek medical advice.
  • Ingestion: rinse mouth; seek medical advice.
• Spill/accidental release: avoid dust; collect with inert absorbent; dispose in accordance with local regulations.
• Waste: treat as organic laboratory chemical waste; follow institutional and regulatory disposal procedures.

Note: Specific toxicological and ecotoxicological information should be obtained from the SDS for AZD1208.

Solvent Selection

AZD1208 is a hydrophobic, heteroaromatic small molecule commonly handled as a concentrated DMSO stock for screening.

• Primary solvent for stocks: DMSO (high-boiling, polar aprotic; excellent solubilizer for kinase inhibitors). Typical stock concentrations: 10–50 mM (general guidance; adjust based on actual solubility from CoA/tests).
• Alternative solvents (general): DMF, NMP, or ethanol may dissolve AZD1208 to varying degrees; test for stability and assay compatibility. For LC–MS, acetonitrile with 0.1% formic acid is commonly used in mobile phases for detection/quantitation.
• Aqueous work: Dilute DMSO stocks into assay buffer immediately before use. Maintain final DMSO at the lowest workable level (e.g., ≤0.5–1% v/v) to minimize effects on protein stability and cells.
• Solvent selection for analytics:
  • HPLC: water/acetonitrile or water/methanol gradients with volatile modifiers (formic acid or ammonium formate) support MS detection.
  • Stability: assess compound stability in chosen solvent over time (freeze–thaw, bench-top stability, and light exposure). Some kinase inhibitors can slowly degrade in basic media—prefer neutral to slightly acidic conditions for storage and analysis.

Note: Exact solubility, extinction coefficients, and pKa are not specified for this item; verify empirically and consult CoA/Spec Sheet.

Storage and Reconstitution

• Storage conditions (Product Data): Store at -80°C.
• Shipping (Product Data): Shipped on dry ice packs + cold packs to maintain low temperature.
• Appearance: Not specified for this item; refer to CoA/Spec Sheet.

Reconstitution (general guidance for research use):

• Solvent: Use anhydrous DMSO to prepare concentrated stock solutions (e.g., 10–50 mM). If another solvent is preferred (DMF, ethanol), confirm compatibility with intended assays.
• Procedure: Allow container to equilibrate briefly to reduce condensation, open under dry conditions, and weigh quickly. Add solvent to achieve desired concentration; vortex and, if needed, brief sonication. Optionally, sterile-filter through 0.22 μm PTFE for cell-based work.
• Aliquoting: Dispense into single-use aliquots to avoid repeated freeze–thaw cycles.
• Storage of solutions: Store aliquots at -80°C (preferred) or -20°C for short term. Protect from light if the compound is photosensitive (verify on CoA/SDS). Record preparation date and concentration.
• Stability: Solution stability and solid-state stability are not specified for this item; perform periodic QC (e.g., LC–MS/HPLC) to confirm integrity.

Always consult the CoA/Spec Sheet and SDS for item-specific handling, stability, and storage details.

Structure and Identity

• Product name: AZD1208 (SKU: A420898)
• CAS: 1204144-28-4
• PubChem CID: 58423153
• InChIKey (from Product Data): 215747 (note: this does not follow the canonical 27-character InChIKey format; consult CoA/SDS for the exact identifier)
• Molecular formula: Not specified for this item; refer to CoA/Spec Sheet.
• Molecular weight: Not specified for this item; refer to CoA/Spec Sheet.
• SMILES: Not specified for this item; refer to CoA/Spec Sheet.

Structural features (general, literature description):

• AZD1208 is a small-molecule, heteroaromatic kinase-inhibitor chemotype designed as a pan-PIM kinase inhibitor (literature/probe information). It typically features multiple nitrogen-rich heterocycles arranged to engage the ATP-binding site via hydrogen-bonding and π-stacking interactions.
• 2D structure (verbal description, literature): an aromatic core linked to one or more azine/azole motifs that present H-bond acceptors toward the hinge region, with appended aryl/heteroaryl substituents that occupy hydrophobic pockets. Stereochemistry is generally not a driver (commonly an achiral scaffold).

Notes:

• The definitive structure identifiers (SMILES/InChI/InChIKey) and empirical formula for this specific lot should be confirmed from the CoA/Spec Sheet or authoritative databases. The above descriptive features are provided as general literature context for AZD1208 as a research chemical probe.

Synthetic Utility

AZD1208 is a finished small-molecule tool compound and is not typically employed as a reagent or synthon in organic synthesis.

Context (general):

• Chemically, AZD1208 comprises nitrogen-rich heteroaromatic motifs optimized for kinase hinge binding. Such scaffolds are typically assembled via heteroaryl–heteroaryl couplings, nucleophilic aromatic substitutions, or amide/urea formations during route development. However, once assembled, the molecule’s functionality is not commonly leveraged as a building block for further synthesis.

Practical guidance for chemists handling the compound:

• If analog generation or SAR expansion is desired, synthetic routes should target upstream intermediates (e.g., halogenated heteroaryl precursors, protected diamines) rather than derivatization of AZD1208 itself.
• For analytical derivatization (e.g., isotopic labeling for internal standards), route redevelopment is typically required at the intermediate stage rather than post-synthesis modification of the final inhibitor.

Conclusion: Treat AZD1208 as a terminal probe compound suited for biological and analytical studies, not as a general-purpose reagent.

Target Specificity

Only information from Product Data may be listed here.

• Item-specific target, clone, epitope, species reactivity: Not specified for this item; refer to CoA/Spec Sheet.

General note: Although AZD1208 is widely cited in the literature as a pan-PIM kinase inhibitor, this section lists only product-specific target validation, which has not been provided in the Product Data.