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BHQ-1 amine is an excellent building block for labeling carbonyl-containing molecules. BHQ-1 dyes are nearly non-fluorescent with moderate extinction coefficients. These dark quenchers are most typically used in Molecular Beacon probes in which FRET has minimal contribution to their fluorescence quenching, thus dependence on donor-acceptor spectral overlap is not significant as to other FRET probes such as FRET peptides. BHQ-1 dyes can quench a variety of fluorophores, in particular, fluorescein derivatives such as FAM, HEX, TET, NED and JOE. This may reflect the BHQ-1's ability to form stable, non-fluorescent complexes with a fluorophore.
| CAS | 1308657-79-5 |
|---|---|
| Especificações e pureza | ≥99% |
| Condições de armazenamento de armazenamento | Store at -20°C |
| Enviado em | Ice chest + Ice pads |
| Product Description | BHQ-1 amine is an excellent building block for labeling carbonyl-containing molecules. BHQ-1 dyes are nearly non-fluorescent with moderate extinction coefficients. These dark quenchers are most typically used in Molecular Beacon probes in which FRET has minimal contribution to their fluorescence quenching, thus dependence on donor-acceptor spectral overlap is not significant as to other FRET probes such as FRET peptides. BHQ-1 dyes can quench a variety of fluorophores, in particular, fluorescein derivatives such as FAM, HEX, TET, NED and JOE. This may reflect the BHQ-1's ability to form stable, non-fluorescent complexes with a fluorophore. |