BI8622 - 10mM in DMSO , CAS No.1875036-74-0

CAS: 1875036-74-0 Cat. No.: B655041 Fórmula: C₂₅H₂₆N₆O Peso molecular: 426.51 PubChem CID: 138377590

GRADE & PURITY 10mM in DMSO

Storage

Store at -80°C

Shipped In

Dry ice packs + Cold packs

Visão geral

BI8622 is a specific inhibitor of the ubiquitin ligase HUWE1 with an IC 50 of 3.1 μM

In Vitro

BI8622 induces HUWE1 ectopically expresses to abolish ubiquitination of MCL1 with an IC 50 value of 6.8 μM in HeLa cells. BI8622 suppresses colony formation of Ls174T cells with estimated IC 50 value of 8.4 μM. BI8622 (10 μM; 1-4 days) treatment retards passage of Ls174T cells through all phases of the cell cycle, with the effect being strongest for G1. BI8622 (0-50 μM; 16 hours) retards the degradation of MCL1 in response to UV irradiation by inhibiting HUWE1 in HeLa cells. BI8622 inhibits MYC-dependent transactivation in colorectal cancer cells. MCE has not independently confirmed the accuracy of these methods. They are for reference only. Cell Cycle AnalysisCell Line: Ls174T cells Concentration: 0 μM, 5 μM,10 μM, 15 μM, 20 μM Incubation Time: 0-4 days Result: Retarded passage of Ls174T cells through all phases of the cell cycle, with the effect being strongest for G1. Western Blot AnalysisCell Line: HeLa cells Concentration: 0 μM, 10 μM, 20 μM Incubation Time: 16 hours Result: Retarded the degradation of MCL1 in response to UV irradiation by inhibiting HUWE1 in HeLa cells.

IC50& Target:IC50: 3.1 μM (HUWE1)

Specifications

Especificações e pureza

10mM in DMSO

Mecanismos bioquímicos e fisiológicos

BI8622 is a specific inhibitor of the ubiquitin ligase HUWE1 with an IC 50 of 3.1 μM.

Condições de armazenamento de armazenamento

Store at -80°C

Enviado em

Dry ice packs + Cold packs

Este produto requer transporte de cadeia fria. Serviços terrestres e outros serviços econômicos não estão disponíveis.

Tipo de ação

INHIBITOR

Nomes e identificadores

• PubChem CID: 138377590
• Peso molecular: 426.51