GRAU & PUREZACarrier Free?Carrier-free — supplied without added carrier protein/stabilizer. Use when carriers (e.g. BSA) would interfere with conjugation or sensitive assays.ExactAb™?ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications.Azide Free?Azide-free — without sodium azide preservative. Use in conjugations, cell work, or assays where azide is toxic or inhibitory.Validated?Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated.High Performance?High-performance grade with optimized purity and performance characteristics. Use for sensitive analyses where ordinary grades fall short.PBS Only?PBS-only formulation — supplied in phosphate-buffered saline with no other additives. Use when you need a clean PBS buffer free of stabilizers/carriers.≥95%(SDS-PAGE)1.0 mg/mL
Carrier Free,ExactAb™,Azide Free,Validated,High Performance,PBS Only,≥95%(SDS-PAGE),1.0 mg/mL Azide Free,Carrier Free,ExactAb™,High Performance,PBS Only,Validated para fluxos de trabalho cromatográficos e analíticos sensíveis que exigem interferência mínima da linha de base.
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Armazenamento e envio
Store at -20°C,Avoid repeated freezing and thawing Envio Ice chest + Ice pads. Consulte o COA específico do lote para obter as especificações exatas.
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Documentos de qualidade
SDS, COA, ficha técnica e folha de especificações disponíveis para download. COA específico do lote acessível por meio de pesquisa por número de lote.
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Comprovação documental
Citado em 0 publicações revisadas por pares nas áreas de cromatografia, síntese orgânica e reações de acoplamento cruzado.
Visão geral
Mouse anti Human Cathepsin D Antibody, clone AB01/1E2, could be used for WB, Flow Cytometry, IF/ICC, ELISA and so on.
Especificações
Product Name
Cathepsin D Mouse mAb - Primary antibody, specific to Human Cathepsin D
Sinónimos
CatD antibody | CATD_HUMAN antibody | Cathepsin D antibody | Cathepsin D heavy chain antibody | CD antibody | Ceroid lipofuscinosis neuronal 10 antibody | CLN10 antibody | CPSD antibody | ctsd antibody | Epididymis secretory sperm binding protein Li 130P
Acid protease active in intracellular protein breakdown. Plays a role in APP processing following cleavage and activation by ADAM30 which leads to APP degradation.
Involved in the pathogenesis of several diseases such as breast cancer and possibly Alzhei
Espécie hospedeira
Mouse
Especificidade
Human Cathepsin D
Imunogénio
Recombinant protein corresponding human Cathepsin D
Azide Free, Carrier Free, ExactAb™, High Performance, PBS Only, Validated
Tipo de corrente de luz
kappa
SDS-PAGE
150.0 KDa
Método de purificação
Protein A purified
Concentração
1.0 mg/mL
Product Line
Produtos biológicos
Armazenamento e envio
Condições de armazenamento de armazenamento
Store at -20°C,Avoid repeated freezing and thawing
Enviado em
Ice chest + Ice pads
Estabilidade e armazenamento
Store at 2-8℃ short term (1-2 weeks). Store at -20℃ long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.
Imagens
Cathepsin D Mouse mAb (Ab215111) - Western Blot All lanes: Cathepsin D Mouse Antibody (Ab215111) at 1/500 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution
Predicted band size: 45 kDa Observed band size: 29 kDa Exposure time: 60.0 s
Cathepsin D Mouse mAb (Ab215111) - Flow Cytometry Overlay histogram showing MCF-7 cells stained with Cathepsin D Mouse mAb (Ab215111) (Red) at 1.25 μg/mL for 1 hour at 4°C. The Goat Anti-Mouse IgG H&L (PE) (Ab179006) was used as the secondary antibody. Unlabeled sample (Black) was used as a negative control. Mouse IgG1-kappa, anti-E8L (Ab213557) was used as an isotype control (Blue).
Cathepsin D Mouse mAb (Ab215111) - IF IF analysis of Cathepsin D Mouse mAb (Green) in MCF-7 cells. The cells were fixed with 4% PFA for 15 minutes and permeabilized with 0.3% Triton X-100 for 15 minutes at RT. Then the cells were stained with Cathepsin D Mouse mAb (Ab215111) at 1.0 μg/mL in blocking buffer for 2 hour at RT and then incubated with Goat Anti-Mouse IgG H&L (AF647) (Ab181952) at a dilution of 1/1000 for 1 hour at room temperature in the dark. Cells were counterstained with DAPI (Blue). Images were taken on the confocal laser scanning microscope.
Aplicação
Aplicação
Dilution info
IF/ICC
1:1000
Flow Cytometry
1/200–1/5000
Documentação
📋 Ficha de dados de segurança (FDS)
Documento abrangente sobre perigos, manuseamento, armazenamento e conformidade regulamentar.
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Dilution Calculator
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Reconstitution Calculator
Revisões
Avaliações dos Clientes
Perguntas frequentes
What is the purity of this product, and how is it determined?
This product is supplied at ≥95% purity, determined by SDS-PAGE. Lot-specific values are stated on the Certificate of Analysis.
What does ExactAb? mean?
ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.
What does Carrier Free mean?
Carrier Free indicates that the material has been processed and tested for specialty industrial processing. Specifications focus on the impurities that affect performance in that application — for example, metal content for electronics, particle size for batteries, refractive index for optics, or moisture content for moisture-sensitive processes.
How should this product be stored?
Store at ?20 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.
How is this product shipped?
This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.
What documentation is provided?
Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.
Which species reactivity has this antibody been validated against?
Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (Cathepsin D Mouse mAb); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.
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