Cellulase Activity Assay Kit (Anthrone, Micro Method)

Cat. No.: C1527443

GRADE & PURITY BioReagent

Overview

Cellulase (CL, EC 3.2.1.4) is widely distributed in bacteria, fungi and animals. It can catalyze the degradation of cellulose and represents a class of enzyme preparations with extensive applications in pharmaceuticals, food, textile, environmental protection, renewable resource utilization and other fields. This kit uses the anthrone colorimetric method to determine CL activity: CL catalyzes the hydrolysis of sodium carboxymethyl cellulose (CMC-Na) to produce reducing sugars. Under the action of concentrated sulfuric acid, reducing sugars are dehydrated to form furan compounds, which react with anthrone reagent to yield a blue-green product with a characteristic absorption peak at 620 nm. The enzymatic activity of CL can be quantitatively calculated by measuring the increasing rate of absorbance at 620 nm.

Storage and Shipping

Storage

Store at 2-8°C

Shipped In

Wet ice