D-Luciferin potassium - for Cell culture, ≥99.9%(HPLC), ee ≥99.9%; Endotoxins ≤0.1 EU/mg, used for Imaging analysis of luciferase-labeled genes and luciferase-fusion genes expressed in vivo/in vitro in living cells, tissues, or organisms; widely used in r

CAS: 115144-35-9 Cat. No.: D1375455 Fórmula: C₁₁H₇KN₂O₃S₂ Peso molecular: 318.41 Beilstein Registry Number: 5416262

GRADE & PURITY for Cell culture, ≥99.9%(HPLC), ee ≥99.9%; Endotoxins ≤0.1 EU/mg

Synonyms

D-Luciferin | Potassium Salt | D-Luciferin potassium | D-(-)-Luciferin potassium | Firefly luciferin potassium | Beetle Luciferin potassium | 4,5-Dihydro-2-(6-hydroxy-2-benzothiazolyl)-4-thiazolecarboxylic acid potassium salt | D-Luciferin potassium salt

Storage

Protected from light,Store at -20°C,Desiccated

Shipped In

Ice chest + Ice pads

Application

ATP Assay, Bioluminescence, Cell Viability Assay, In Vivo Imaging, Reporter Gene Assay

Visão geral

Tested and Certified for in vivo imaging. 

Luciferin is a common bioluminescent reporter used for in vivo imaging of the expression of luciferase. This water soluble substrate for the firefly luciferase enzyme utilizes ATP and Mg2+ as cofactors to emit a characteristic yellow-green emission in the presence of oxygen, which shifts to red light in vivo at 37°C. Through the utilization of ATP, the reaction can be further used to indicate the presence of energy or life in order to function as a life-death stain.

Luciferin is a common reagent used throughout the biotechnology field and specifically for in vivo imaging. Luciferase labeled tumor cells, stem cells or infectious diseases are often inoculated into research animals such as rats or mice for investigation. The injection of luciferin allows for the real-time, noninvasive monitoring of disease progression and/or drug efficacy in these model systems through Bioluminescence Imaging (BLI).

Luciferin is also commonly used for in vitro research, including luciferase and ATP assays, gene reporter assays, high throughput sequencing and various contamination assays.

Specifications

Sinónimos

D-Luciferin | Potassium Salt | D-Luciferin potassium | D-(-)-Luciferin potassium | Firefly luciferin potassium | Beetle Luciferin potassium | 4,5-Dihydro-2-(6-hydroxy-2-benzothiazolyl)-4-thiazolecarboxylic acid potassium salt | D-Luciferin potassium salt

Especificações e pureza

for Cell culture, ≥99.9%(HPLC), ee ≥99.9%; Endotoxins ≤0.1 EU/mg

Aplicação

Imaging analysis of luciferase-labeled genes and luciferase-fusion genes expressed in vivo/in vitro in living cells, tissues, or organisms; widely used in reporter gene analysis, immunoassay, and ATP fluorescence hygiene monitoring analysis

Estabilidade e armazenamento

Store at -20℃ long term (2 years). Store in the dark. Desiccated.

Condições de armazenamento de armazenamento

Protected from light,Store at -20°C,Desiccated

Enviado em

Ice chest + Ice pads

Este produto requer transporte de cadeia fria. Serviços terrestres e outros serviços econômicos não estão disponíveis.

Grau

for Cell culture

Pureza

≥99.9%(HPLC)

Nomes e identificadores

Sorrisos canónicos

C1C(N=C(S1)C2=NC3=C(S2)C=C(C=C3)O)C(=O)[O-].[K+]

IUPAC Name

potassium;(4S)-2-(6-hydroxy-1,3-benzothiazol-2-yl)-4,5-dihydro-1,3-thiazole-4-carboxylate

InChIKey

UMBKGTQQGYPQBE-OGFXRTJISA-M

INCHI

1S/C11H8N2O3S2.K/c14-5-1-2-6-8(3-5)18-10(12-6)9-13-7(4-17-9)11(15)16;/h1-3,7,14H,4H2,(H,15,16);/q;+1/p-1/t7-;/m1./s1

WGK Alemanha

3

Peso molecular

318.41

Beilstein

5416262

Reaxy-Rn

41031268

Reaxys-RN_link_address

https://www.reaxys.com/reaxys/secured/hopinto.do?context=S&query=IDE.XRN=41031268&ln=

Propriedades químicas e físicas

• Solubilidade: No less than 40mg/mL in PBS or pure water