BioReagent BioReagent for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
Room temperature Ships Normal Check lot-specific COA for exact specifications.
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Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
The purpose of fixation is to preserve the original morphological structure of cells and tissues. Fixatives prevent autolysis of tissues and cells triggered by endogenous lysosomal enzymes and suppress the growth of bacteria and fungi. Through coagulation and adduct formation, fixatives alter the internal structure of proteins to inactivate enzymes and induce physical changes to cell nuclei and extracellular components. Fixatives are mainly classified into aldehyde, mercury, alcohol, oxidant and picrate categories, among which formalin (aldehyde type) and ethanol (alcohol type) are widely adopted.
Davidson's Fixative, also named Hartmann's Fixative, is composed of 15% ethanol, 5% acetic acid, formaldehyde and deionized water. It contains no dyes, mercury or other heavy metals. As a rapid fixative, it delivers excellent nuclear preservation, generates minimal formalin pigment, causes low tissue shrinkage and retains superior morphological details. It is applicable to routine histopathological examinations, especially for tumors, bone marrow, gynecological specimens, fatty breast tissues and biopsies. It can also be used for overnight fixation of testicular biopsies and fatty lymph nodes. Moreover, Davidson's Fixative is the preferred fixative for whole-eye specimens, providing optimal tissue preservation and preventing retinal detachment. It is widely used for rapid dehydration, routine fixation and fast fixation for frozen sections, and can serve as a preservative for tissue samples. This reagent is for research use only and not intended for clinical diagnosis.
Operating Procedures (For Reference Only)
1.Immerse fresh tissues in Davidson's Fixative and fix at room temperature for 1–2 days. Fixation of small samples shall not exceed 24 hours. For specimens intended for subsequent immunohistochemistry, fixation time shall be within 24 hours; for routine histopathological tests, fixation shall not exceed 48 hours.
2.Take tissue specimens out of Davidson's Fixative and rinse briefly with tap water.
3.After fixation, store samples in 10% formalin or 70% ethanol.
Precautions
1.This fixative contains formaldehyde which is harmful to human health. Operate cautiously in a well-ventilated area and avoid inhalation of vapors.
2.Fixation time varies with tissue thickness. Proper trimming facilitates penetration of fixative. The optimal thickness of routine biopsy tissues is 2–4 mm and shall not exceed 6 mm.
3.Use sufficient volume of fixative; the volume ratio of fixative to tissue block shall be greater than 10:1. Replace the fixative 1–3 times during fixation if the volume is insufficient.
4.Temperature has a remarkable impact on fixation. Higher temperature accelerates fixation, yet excessive temperature must be avoided.
5.Fix fresh tissues immediately after dissection.
6.For hard specimens such as eyeballs, store in 70% ethanol for 1 day after fixation, then transfer to 90% ethanol for another day, followed by absolute ethanol for 1 day to improve sectioning performance.
7.All fixation operations must be carried out in a fume hood with lab coat and disposable gloves worn for personal safety.
8.Use the reagent promptly after opening to avoid compromised experimental results.
Comprehensive hazard, handling, storage, and regulatory compliance document.
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| Lot Number | Certificate Type | Data | Item |
|---|---|---|---|
 ZJ26F0939292 | Certificate of Analysis | Sep 17, 2026 | D1526176 |
| ZJ26F0939291 | Certificate of Analysis | Sep 17, 2026 | D1526176 |
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