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Revisões
Avaliações dos Clientes
Application Protocols
Only product‑provided protocols can be listed here.
Tested applications, recommended transfection conditions, working concentrations, and positive control references: Not specified for this item; refer to CoA/Spec Sheet and SDS.
Notes
As application conditions for siRNA are cell‑type dependent, users should consult the product datasheet when available and follow institutional best practices for RNA handling and transfection optimization.
Biological Roles
Target context (general literature; not item‑specific)
Gene: Ddr2 (Discoidin Domain Receptor 2) encodes a receptor tyrosine kinase activated by fibrillar collagens. It plays roles in extracellular matrix (ECM) sensing, cell adhesion, and regulation of matrix remodeling.
Signaling: Upon collagen engagement, DDR2 undergoes autophosphorylation and triggers downstream pathways (e.g., MAPK cascades), modulating gene expression programs associated with ECM turnover and cell motility.
Expression: DDR2 is detected in various mesenchymal and stromal cell types in mouse tissues; exact expression levels are cell‑type and context dependent.
Relevance to siRNA knockdown studies
Perturbing Ddr2 expression enables dissection of collagen‑dependent signaling networks, evaluation of cross‑talk with integrins, and examination of transcriptional outputs that regulate ECM components (e.g., collagens, matrix metalloproteinases).
Endpoint readouts often include: mRNA quantification (qPCR), protein levels (western blot), receptor phosphorylation status, and functional assays such as migration on collagen matrices. Specific anticipated phenotypes depend on model system and are outside the scope of product‑specific claims.
Note
The above biological roles are literature‑based context for experimental design and do not constitute product performance specifications.
Buffer Applications
Not typically applicable.
This product is an siRNA reagent rather than a buffering agent. While siRNAs are handled in aqueous buffers, they do not function as buffers themselves. For guidance on suitable diluents, see the Solvent Selection and Storage & Reconstitution tabs.
Green Alternatives
Context
Green chemistry comparisons are not directly applicable to an siRNA reagent. However, strategic use of RNAi can reduce resource‑intensive steps in research.
Considerations (general; not item‑specific)
siRNA vs genetic knockout: Transient RNAi can obviate the need for generating multiple stable knockout lines for exploratory studies, potentially decreasing reagent, plastic, and energy consumption.
siRNA vs small‑molecule inhibitors: When selective inhibitors are unavailable or require hazardous solvents, RNAi offers an aqueous, target‑directed perturbation, though it entails transfection reagents and cell culture plastics.
Good laboratory practices to reduce environmental impact
Scale experiments appropriately; consolidate transfections to minimize consumables.
Use aqueous buffers, avoid unnecessary organic solvents in downstream analyses, and dispose of waste per institutional sustainability guidelines.
Pharmaceutical Uses
Not applicable.
This product is designated for research use only. It is not formulated as a drug substance or excipient, and there are no pharmacopeial specifications provided for this item.
Physical Properties
Item-specific facts (from Product Data)
Physical form/appearance: Not specified for this item; refer to CoA/Spec Sheet.
Molecular weight (per duplex/strand): Not specified for this item; refer to CoA/Spec Sheet.
Solubility: Not specified for this item; refer to CoA/Spec Sheet.
General/literature values for siRNA (not item-specific)
Typical length: 19–21 base pairs (plus 2‑nt 3′ overhangs).
Approximate molecular mass: ~13–14 kDa per 21‑mer duplex (sequence‑dependent).
Solubility: Freely soluble in RNase‑free water and common biological buffers (e.g., 1× TE, 10 mM Tris‑HCl, pH ~7.5–8.0) to high mM concentrations; best practice is to use nuclease‑free solutions.
Charge/pKa: Strongly polyanionic due to phosphate backbone; no discrete pKa relevant to bulk solubility.
UV characteristics: Strong absorbance at 260 nm (A260) with sequence‑dependent extinction coefficients; quantification typically via A260 (literature practice).
Notes
Melting temperature (Tm) of siRNA duplexes depends on length, GC content, and salt; exact values are sequence‑specific and not specified for this item.
Quality and Grades
Item-specific details (from Product Data)
Grade/purity: Not specified for this item; refer to CoA/Spec Sheet.
Format/appearance: Not specified for this item; refer to CoA/Spec Sheet.
General expectations for pre‑designed siRNA sets (literature/industry practice; not item‑specific)
Pre‑designed set concept: Multiple independent siRNA duplexes targeting distinct regions of the same gene (here: mouse Ddr2) to increase likelihood of robust knockdown and to enable pooled or deconvoluted validation.
Typical QC suite: Mass confirmation (e.g., MALDI‑TOF), analytical HPLC for purity, OD260 quantitation, and, where applicable, endotoxin testing for cell‑based work. Exact QC metrics for this item are not specified.
Purification levels: Desalted, cartridge‑purified, or HPLC‑purified are common; higher purity reduces off‑targets from truncated products. Specific purification for this item is not specified.
Stabilizers/modifications: Some siRNAs include chemical stabilizations (2′‑O‑Me, 2′‑F, PS) to enhance nuclease resistance; presence/absence for this item is not specified.
How to use this information
Consult the product’s CoA/Spec Sheet for actual purity, yield (nmol/OD260), modification status, and recommended use (pool vs individual duplexes). Use at consistent OD‑based dosing to normalize across duplexes when comparing effects.
Reaction and Applications
Applicability
This section addresses experimental applications rather than chemical reactions, as the product is an siRNA set.
Typical research applications for Ddr2‑targeting siRNA (literature; not item‑specific)
Transient gene knockdown in mouse cell lines or primary cells expressing Ddr2 to study receptor signaling, ECM interactions, and downstream transcriptional programs.
Phenotypic assays following knockdown: alterations in collagen‑dependent signaling readouts, changes in cell migration or adhesion on collagen substrates, and modulation of kinase pathway activation (e.g., ERK/JNK) downstream of DDR2. Assays and endpoints are user‑defined.
Use formats: Individual duplex testing to identify best performers; pooled duplexes to maximize knockdown robustness and minimize off‑target idiosyncrasies.
Delivery methods: Lipid‑mediated transfection, electroporation, or nanoparticle‑based delivery optimized for the chosen mouse cell model. Specific protocols for this item are not provided; see product datasheet/CoA.
Practical tips (general)
Include non‑targeting and positive control siRNAs; monitor knockdown at mRNA (qPCR) and protein (immunoblot) levels at multiple timepoints (24–96 h). Optimize dose to minimize cytotoxicity while achieving target suppression.
Reaction Conditions
Not applicable in the chemical sense.
Conventional reaction parameters (solvent, catalyst, temperature) for small‑molecule transformations do not pertain to siRNA use. For experimental considerations related to transfection or electroporation, consult validated cell biology protocols and the product’s datasheet. Specific conditions for this item are not provided in the Product Data.
Safety and Handling
Item-specific safety (from Product Data)
GHS classification, signal word, hazard statements, pictograms: Not specified for this item; refer to SDS.
Research use only; not for human or veterinary use.
General handling guidance for siRNA (literature/practice; defer to SDS for authoritative instructions)
PPE: Laboratory coat, gloves, and safety glasses. Work in a clean area to prevent RNase contamination.
Incompatibilities: Avoid RNase contamination from skin, dust, or non‑sterile plastics; use RNase‑free tubes, tips, and water. Avoid repeated freeze–thaw cycles which can degrade RNA.
Environmental/ventilation: Normal laboratory ventilation is generally adequate for aqueous siRNA handling; avoid aerosol generation when lyophilized material is present.
First‑aid (general): If contacted with eyes/skin, rinse with water. If inhaled (powder), move to fresh air. If ingested, rinse mouth. Seek medical advice as appropriate. Always consult SDS for product‑specific measures.
Spill response: For dry material, gently dampen to minimize dust and collect with RNase‑free tools. For solutions, wipe with RNase decontamination agents or 70% ethanol, dispose per institutional guidelines.
Waste: Dispose of nucleic acid waste following local regulations and biosafety policies.
Storage‑related precautions
Keep tightly capped, desiccated if lyophilized, and protected from repeated warming/cooling. Store at the specified temperature (see Storage & Reconstitution tab).
Solvent Selection
Applicability
This product is an siRNA oligonucleotide set, not a solvent. Conventional solvent selection guidance is not directly applicable.
General guidance for reconstitution/dilution of siRNA (literature; not item‑specific)
Primary diluent: RNase‑free water is commonly used for initial reconstitution to a high‑concentration stock (e.g., 20–100 µM), then diluted in RNase‑free buffers for working solutions. Specific concentrations for this item are not specified.
Compatible buffers: Low‑ionic strength, slightly basic buffers (e.g., 10 mM Tris‑HCl, pH ~7.5–8.0; TE with low EDTA) help maintain stability; avoid divalent cations and RNases.
Additives: For storage aliquots, some labs include RNase inhibitors or small amounts of sterile NaCl to match transfection mixes; practice varies and is application‑dependent.
Comparison notes
Water vs TE: Water is simplest and least interfering; TE can enhance stability during long‑term storage, but EDTA may interact with transfection reagents—validate in your system.
Storage and Reconstitution
Item-specific instructions (from Product Data)
Storage: Store at −20°C.
Shipping: Shipped in an ice chest with ice pads to maintain low temperature during transit.
Not specified for this item; refer to CoA/Spec Sheet
Reconstitution solvent, target concentration, and aliquotting recommendations
Long‑term stability claims at −20°C/−80°C and number of permissible freeze–thaw cycles
Presence of stabilizers or lyophilized buffer composition (if applicable)
General stewardship notes (for user planning; not item‑specific)
Protect from RNases: Use nuclease‑free tubes, tips, and water; wear gloves and clean work surfaces.
Minimize freeze–thaw: Prepare single‑use aliquots upon first thaw/reconstitution to preserve integrity.
Light/moisture: Store tightly capped; desiccate lyophilized material if applicable. Keep solutions chilled during handling.
Structure and Identity
Item-specific facts (from Product Data)
Product: Ddr2 Mouse Pre-designed siRNA Set A (SKU: D1471361)
Composition: Short double‑stranded RNA duplexes, typically 19–21 bp with 2‑nt 3′ overhangs; one strand (guide) is antisense to the target mRNA, the other (passenger) facilitates RISC loading.
Backbone/chemistry: Phosphodiester or modified phosphoramidate backbones with ribose sugars; may include stabilizing modifications (e.g., 2′‑O‑Me, 2′‑F, PS linkages) in some products; specific modifications for this item are not specified.
Structural features: Linear, polyanionic duplexes with Watson–Crick base pairing; no fixed 3D tertiary structure in solution beyond A‑form RNA duplex character.
2D depiction (verbal): Two antiparallel RNA strands (5′→3′ and 3′→5′), complementary bases paired along ~19–21 nucleotides, typically with 3′ overhangs (e.g., “dTdT”), phosphodiester linkages between ribonucleotides.
Notes
As an oligonucleotide mixture/set, conventional small‑molecule identifiers (SMILES/InChI) are generally not applicable.
Synthetic Utility
Not applicable.
As a biological oligonucleotide reagent, this product is not used as a building block or reagent in synthetic organic chemistry. Users interested in small‑molecule synthesis should refer to appropriate chemical reagents.
Target Specificity
Item-specific targeting (from Product Data/name)
Target gene: Ddr2 (Discoidin Domain Receptor 2)
Species: Mouse (Mus musculus)
Product format: Pre‑designed siRNA Set A (multiple duplexes targeting the same gene; exact sequences and count are not specified for this item).
Not specified for this item; refer to CoA/Spec Sheet
Individual siRNA sequences and target exons
Validated cell lines/tissues
Knockdown efficiency data and off‑target assessment
Any proprietary chemical modifications or pooling strategy
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