EB Solution (1 mg/mL) - BioReagent, Biological Stain, for NA electrophoresis, 1 mg/ml in DMF , CAS No.1239-45-8

CAS: 1239-45-8 Cat. No.: E1526367 Fórmula: C₂₁H₂₀BrN₃ Peso molecular: 394.32 Número CE: 214-984-6

GRAU & PUREZA BioReagent, Biological Stain, for NA electrophoresis, 1 mg/ml in DMF

Synonyms

1 mg/ml Ethidium Bromide Aqueous Solution

Storage

Protected from light,Room temperature

Shipped In

Normal

Application

Cell Staining, Nucleic Acid Electrophoresis

Visão geral

Ethidium Bromide (molecular formula: C₂₁H₂₀BrN₃, molecular weight: 394.31) is a highly sensitive fluorescent dye used for visualizing DNA in agarose and polyacrylamide gels. It emits an orange-red fluorescence signal when excited by a 302 nm UV transilluminator. EB is toxic; all EB-containing solutions must be decontaminated before disposal to prevent environmental pollution. EB Solution (1mg/mL) is prepared with sterile double-distilled water and is widely applied in electrophoresis of DNA and RNA samples.

Required Materials:

1. Electrophoresis running buffer (e.g., TAE, TBE)

2. Microwave oven

3. UV analyzer or gel documentation imaging system

Precautions:

1. Gel thickness should not exceed 0.5 cm; excessive thickness will reduce detection sensitivity.

2. Avoid prolonged UV exposure when recovering DNA fragments via gel electrophoresis.

3. EB is toxic and irritates the skin and eyes. Handle with extreme caution.

4. For personal safety, wear a lab coat, disposable gloves and a face mask during operation.

5. This product is for scientific research use by qualified professionals only. It must not be used for clinical diagnosis or treatment, food or pharmaceutical production, nor stored in residential premises.

Especificações

Sinónimos

1 mg/ml Ethidium Bromide Aqueous Solution

Especificações e pureza

BioReagent, Biological Stain, for NA electrophoresis, 1 mg/ml in DMF

Estabilidade e armazenamento

Store at room temperature long term (12 months). Store in the dark.

Condições de armazenamento de armazenamento

Protected from light,Room temperature

Enviado em

Normal

Grau

Biological Stain, BioReagent, for NA electrophoresis

Product Line

Produtos biológicos

Nomes e identificadores

SMILES isoméricas

CC[N+]1=C2C=C(C=CC2=C3C=CC(=CC3=C1C4=CC=CC=C4)N)N.[Br-]

WGK Alemanha

3

RTECS

SF7950000

Peso molecular

394.32

Reaxy-Rn

3642536

Reaxys-RN_link_address

https://www.reaxys.com/reaxys/secured/hopinto.do?context=S&query=IDE.XRN=3642536&ln=

Propriedades químicas e físicas

• Sensibilidade: Light-sensitive
• Ponto de fusão (°C): 260-262°C