Laboratory Protocols & SOPs Library

A Broth (Powder)

· Published 27/07/2023 · Updated 09/06/2025

Directions per Liter:

Dissolve 16.5 grams per liter of distilled/deionized (DDI) water, heating with stirring until completely solubilized, adjusting pH as necessary. Dispense into appropriate containers, loosen caps and autoclave for 15 minutes at 121ºC (15psi).

Component	g/liter
Ammonium Sulfate	1
Potassium Phosphate Dibasic	10.5
Potassium Phosphate Mono	4.5
Sodium Citrate	0.5
	Total: 16.5g/Liter
	500g makes 32 liters

Storage and Stability:

Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept refrigerated and used within a short period of time.

Categories: Laboratory Protocols & SOPs Library

Da — when not otherwise indicated, molecular weight units are daltons. Mw — weight-average molecular weight. Mn — number-average molecular weight.

Products are supplied for research and development use only. Not for use in humans, animals, diagnosis, or therapy.

Cite this article

Aladdin Scientific. "A Broth (Powder)" Aladdin Knowledge Base, updated 09/06/2025. https://www.aladdinsci.com/eu_pt/faqs/a-broth-powder-en.html

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