Amies Broth with Charcoal (Amies Transport Medium) (Powder)

Directions per Liter:

Dissolve 18.05 grams in 800-900ml of ddH2O stirring gently with heating until completely solubilized. Adjust pH of the medium to the desired level. Add additional water to bring the solution to 1L. Dispense into appropriate containers, loosen caps and autoclave for 15 minutes at 121ºC (15psi).

Component	g/liter
Calcium Chloride	0.1
Charcoal	10
Magnesium Chloride	0.1
Potassium Chloride	0.2
Monopotassium Phosphate	2.5
Sodium Chloride	3
Disodium Phosphate	1.15
Sodium Thioglycollate	1
	Total: 18.05g/Liter

Storage and Stability:

Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.