Animal model of benign prostatic hyperplasia

Summary

Benign prostatic hyperplasia (BPH) is a histologic diagnosis that manifests as tissue hyperplasia in the interstitium of the prostate, and clinical BPH usually presents as an anatomically or imaging-confirmed enlargement of the prostate. There are four methods of modeling BPH: estrogen androgen combination method, testosterone propionate injection method, in vitro cellular model, and transgenic method.

Operation method

Combined use of estrogen and male hormones

Principle

Modern research suggests that prostate hyperplasia in middle-aged and elderly men is related to the imbalance of estrogen/androgen ratio in the body, and the use of estrogen and male hormone combination can lead to prostate hyperplasia.

Materials and Instruments

Equipment:
A set of routine surgical instruments, B-mode ultrasound diagnostic instrument, electronic balance, vernier calipers
Reagents:
① anesthesia supplies (such as speed sleep new)
② 5α-androstane-3α ③ 17β-estradiol
③ 17β-estradiol
④ Triglycerides

Move

A. Under anesthesia and asepsis, the testes were surgically removed bilaterally.

B. After 1 week of natural recovery, each one was injected intramuscularly with 1 ml of triglyceride containing 5α-androstane-3α (25 mg) and 17β-estradiol (0.25 mg) each time, 3 times a week for 6 months.

C. Observation of prostate volume, weight, pathologic observation, and mesenchymal epithelial ratio.

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