Brilliant Green Agar (Powder)

Directions per Liter:

Dissolve 58grams per liter of distilled/ deionized (DDI) water, heating with stirring until completely solubilized.

Dispense into appropriate containers, loosen caps and autoclave for 15 minutes at 121ºC (15psi).

Component	g/liter
Casein Digest Peptone	5
Lactose	10
Yeast Extract	3
Brilliant Green	0.0125
Peptic Digest of Animal Tissue	5
Sodium Chloride	5
Sucrose	10
Phenol Red	0.8
Agar	20
	Total: 58g/Liter

Storage and Stability:

Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.