Enterostatin Human: Key Facts, Uses & FAQs

Enterostatin human is a five-amino-acid peptide (Val-Pro-Asp-Pro-Arg) derived from the N-terminal activation peptide of procolipase that selectively suppresses dietary fat intake and serves as a key research tool in appetite and obesity studies.

Product SKU

E118905

Peptide Sequence

Val-Pro-Asp-Pro-Arg (VPDPR)

Molecular Origin

N-terminal pentapeptide cleaved from human procolipase upon tryptic activation

Primary Bioactivity

Selective suppression of high-fat diet intake; implicated in peripheral and central satiety signaling

Species Relevance

Human; sequence is also conserved across several mammalian species

Recommended Storage

-20 °C upon receipt; avoid repeated freeze-thaw cycles

Typical Research Applications

Obesity research, appetite regulation studies, procolipase biology, in vitro receptor binding assays

What Is Enterostatin Human?

Enterostatin human is a pentapeptide with the sequence Val-Pro-Asp-Pro-Arg (VPDPR). It is generated in the gastrointestinal tract when pancreatic procolipase is cleaved by trypsin during fat digestion. The resulting five-residue N-terminal fragment — enterostatin — is released into the intestinal lumen and enters circulation, where it exerts signaling effects related to fat intake regulation.

As a research reagent (SKU E118905), Enterostatin human is supplied as a synthetic peptide intended for laboratory use in studies of appetite control, energy homeostasis, and obesity mechanisms. Its short, defined sequence makes it amenable to in vitro assays, receptor binding studies, and in vivo animal model experiments.

Biological Role and Research Significance

The principal characterized bioactivity of enterostatin is the selective suppression of fat intake. Unlike general appetite suppressants, enterostatin appears to specifically reduce the consumption of high-fat foods without proportionally affecting carbohydrate or protein intake. This selectivity makes it a valuable probe for dissecting the molecular pathways that regulate macronutrient-specific appetite.

Evidence from animal model studies suggests that enterostatin acts through both peripheral (gastrointestinal) and central (brain) mechanisms. Peripheral administration has been shown to reduce fat intake, while central administration implicates serotonergic and opioid pathways in the hypothalamus. Researchers studying the neurobiology of obesity and dietary fat preference employ enterostatin to interrogate these circuits.

Additionally, enterostatin research has implications for understanding post-ingestive feedback signals — the hormonal and peptidergic messages sent from the gut to the brain that help determine meal size and macronutrient selection.

Handling, Reconstitution, and Storage Guidelines

Storage: Upon receipt, Enterostatin human (E118905) should be stored at −20 °C. Lyophilized peptide is stable under these conditions. Avoid exposure to moisture and repeated freeze-thaw cycles, which can degrade peptide integrity over time.

Reconstitution: It is recommended to reconstitute the lyophilized powder in sterile water or an aqueous buffer (e.g., phosphate-buffered saline, pH 7.4) at a concentration appropriate for your assay. For in vivo studies, consult established literature for vehicle and dosing considerations. Allow the vial to equilibrate to room temperature before opening to minimize condensation.

Handling precautions: Use standard laboratory PPE. Although enterostatin is a naturally occurring human peptide with no known acute hazard at research quantities, handle all reagents with appropriate care. Prepare single-use aliquots after initial reconstitution to avoid freeze-thaw degradation.

Note: Specific purity, molecular weight, and lot-specific certificate-of-analysis data should be confirmed in the product documentation provided with SKU E118905.

Common Research Applications

Researchers utilize Enterostatin human (E118905) across several experimental contexts:

• In vivo feeding behavior studies: Peripheral (intraperitoneal, intravenous) or central (intracerebroventricular) administration in rodent models to assess effects on fat intake, meal pattern, and body weight.
• Receptor and binding assays: Identification and characterization of putative enterostatin receptors or binding proteins in intestinal and neural tissue preparations.
• Cell signaling studies: Investigation of downstream intracellular pathways activated by enterostatin, including serotonergic and opioid receptor cross-talk.
• Obesity and metabolic disease research: Evaluation of enterostatin as a potential endogenous anti-obesity signal and its relationship to dietary fat preference and adiposity.
• Procolipase biology: Studies examining the coordinated regulation of procolipase activation and enterostatin release in the context of pancreatic exocrine function and fat digestion.

Quality, Specifications, and Ordering Information

Enterostatin human is available from Aladdin Scientific under SKU E118905. The product is a synthetic human pentapeptide (VPDPR) manufactured for research use. Key specification details — including exact molecular weight, purity grade, counterion, and available pack sizes — are provided in the product datasheet and certificate of analysis accompanying each lot.

For ordering inquiries, bulk pricing, or technical support regarding reconstitution and assay design, please contact Aladdin Scientific customer support or your regional sales representative. When placing an order, reference SKU E118905 to ensure correct product selection.

This product is intended for research use only and is not suitable for diagnostic, therapeutic, or food/drug applications.

Frequently asked questions

What is the amino acid sequence of Enterostatin human?

Enterostatin human consists of five amino acids: Val-Pro-Asp-Pro-Arg (VPDPR). This pentapeptide corresponds to the N-terminal activation peptide released when trypsin cleaves human procolipase during pancreatic fat digestion.

How should I reconstitute and store Enterostatin human (E118905)?

Reconstitute the lyophilized powder in sterile water or aqueous buffer such as PBS (pH 7.4) to your desired working concentration. Store the lyophilized material at −20 °C, and after reconstitution, prepare single-use aliquots to avoid repeated freeze-thaw cycles that may reduce peptide activity and integrity.

What makes enterostatin selective for fat intake rather than overall appetite?

Research indicates that enterostatin selectively reduces high-fat food consumption without proportionally suppressing intake of carbohydrates or proteins, suggesting it acts on macronutrient-specific signaling pathways. The exact molecular basis of this selectivity — potentially involving specific receptor subtypes and serotonergic or opioid pathways — remains an active area of investigation, making Enterostatin human a valuable research tool for these studies.

Is Enterostatin human suitable for in vivo animal studies?

Yes, enterostatin has been used in rodent model studies via peripheral (e.g., intraperitoneal) and central (e.g., intracerebroventricular) routes of administration to study fat intake and appetite regulation. Researchers should consult published literature for appropriate dosing, vehicle selection, and experimental design, and confirm that the purity and formulation of SKU E118905 meet their specific in vivo study requirements.

What is the relationship between procolipase and enterostatin?

Procolipase is the zymogen precursor of colipase, a cofactor required for pancreatic lipase activity during fat digestion. When procolipase is activated by trypsin in the small intestine, the N-terminal pentapeptide VPDPR is cleaved off — this fragment is enterostatin. Thus, enterostatin generation is directly coupled to the activation of the fat-digestion machinery, positioning it as a physiological signal linking fat consumption to satiety feedback.

References

1. Erlanson-Albertsson C, York D. Enterostatin — a peptide regulating fat intake. Obesity Research. 1997;5(4):360-372.
2. Erlanson-Albertsson C. The role of enterostatin in the regulation of food intake and energy expenditure. Recent advances in research. Annals of the New York Academy of Sciences. 1989;575:362-373.
3. Mei J, Erlanson-Albertsson C. Role of enterostatin in the regulation of fat intake in rats. American Journal of Physiology - Regulatory, Integrative and Comparative Physiology. 1996;271(3):R704-R708.

Products are supplied for research and development use only. Not for use in humans, animals, diagnosis, or therapy.