I only have 2.5×10^5 cells in my cell sample, do I only need to use 1.25 μL of flow-through antibody?
Frequently Asked Questions: Products, Experiments and Troubleshooting
I only have 2.5×10^5 cells in my cell sample, do I only need to use 1.25 μL of flow-through antibody?
·Published ·Updated
I only have 2.5×10^5 cells in my cell sample, do I only need to use 1.25 μL of flow-through antibody?
The amount of antibody used is mainly related to the volume of fluid and the number of cells. If the amount of cells is small, the whole system can be halved, that is, 50
μl
cell suspension + 2.5
μl
flow-through antibody. However, if there are very few target cells in the sample, a reduction in the amount of cells may have an effect on the results, resulting in only a small signal being detected.
Da — when not otherwise indicated, molecular weight units are daltons. Mw — weight-average molecular weight. Mn — number-average molecular weight.
Products are supplied for research and development use only. Not for use in humans, animals, diagnosis, or therapy.
Cite this article
Aladdin Scientific. "I only have 2.5×10^5 cells in my cell sample, do I only need to use 1.25 μL of flow-through antibody?" Aladdin Knowledge Base, updated 09/06/2025. https://www.aladdinsci.com/eu_pt/faqs/flow-through-antibody-en.html
Was this article helpful?YesNo41 out 79 found this helpful
Usamos cookies para garantir o funcionamento adequado do site e, quando permitido, para melhorar a sua experiência. Você pode gerenciar suas preferências a qualquer momento nas Configurações. Saiba mais em nossa Política de Cookies.
Deseja que lhe enviemos uma mensagem quando tivermos descontos disponíveis?
Lembrar-me mais tarde
Obrigado! Por favor, verifique a sua caixa de entrada de e-mail para confirmar.
Products are supplied to verified businesses, institutions, and qualified professionals for research and development use only. Not for use in humans, animals, diagnosis, or therapy.
