MH60.BSF2 is an IL-6-dependent cell line, and the amount of MH60.BSF2 cell proliferation was positively compared with the biological activity of IL-6.
Operation method
IL-6 biological activity assay
Materials and Instruments
Induction of IL-6 FCS-RPMI-1640 culture medium Glutamine HEPES Penicillin Streptomycin PBSA buffer Centrifuge Incubator Shaker
Move
1. Induction of IL-6: The induction process is basically the same as IL-2. The cells used to induce IL-6 production can be PBMC. the inducer is PHA.
2. Determination of biological activity of IL-6: MH60.BSF2 cell proliferation assay was used to determine the process IL-2. The concentration of MH60.BSF2 cells applied was 2×105/ml.
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