Dissolve 10.5 grams in 800-900ml of ddH2O stirring gently with heating until completely solubilized. Adjust pH of the medium to the desired level. Add additional water to bring the solution to1L. Dispense into appropriate containers, loosen caps and autoclave for15 minutes at 121ºC (15psi).
Component
g/liter
Ammonium Chloride
1
Potassium Phosphate mono
3
Sodium Chloride
0.5
Sodium Phosphate dibasic
6
Total: 10.5g/Liter
Note: Calcium chloride is not included since it is optional in the preparation of M9. If desired, add 15mg/liter.
Storage and Stability:
Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept refrigerated and used within a short period of time.
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