RNA-mediated gene silencing assay
Down-regulation of endogenous gene expression by post-transcriptional horizontal gene silencing (PTGS) is a key to characterize plant gene function. Many RNA le
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Laboratory Protocols & SOPs Library
3,200+ step-by-step lab protocols and SOPs for cell culture, staining, PCR, protein work and microbiology, with reagents, conditions and evaluation criteria.
3228 articles
Fat Extraction - Soxhlet's Fat Extraction Method Experiment
In the determination of the oil content of the sample, usually using organic solvents with a boiling point of less than 60 ℃ as a fat solvent (such as ether an
Two-dimensional difference gel electrophoresis (DIGE) experiments on tomato leaves and roots
We can analyze the differential expression of proteins in two different tissues and organs of the same plant by two-dimensional fluorescence difference gel elec
Experiments on Plant Damage from Undesirable Environments
When plant tissues are subjected to drought or other adverse conditions, the protoplasmic structure of the cells will be injured, especially the membrane system
Determination of coolant content in plant tissues
Phenolic substances in plant tissues are oxidized to Da by polyphenol oxidase, and Da can automatically polymerize into colorful substances and browning occurs.
Experiments on the effect of potassium ions on stomatal openings
The expansion and contraction of the guard cells on both sides of the stomata directly affects the opening and closing of the stomata, which significantly influ
Determination of stomatal opening and closing conditions in plants
Stomata are the main channel for gas exchange between plant leaves and the outside world, and the distribution, density, shape, size and opening and closing of
Determination of plant transpiration rate
The speed of transpiration is related to the speed of mineral salt transportation in the plant and leaf temperature, etc. The transpiration rate can be used as
Measurement of vitamin C content
Vitamin C is one of the most important vitamins in human nutrition and a deficiency can lead to scurvy. Fruits and vegetables are the main source of vitamin C f