Plate-laying and transfer experiments for stickies and plasmid libraries
The library should be amplified as soon as possible once it is packaged; it can greatly increase the copy number of the library, but there may be some potential
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Experimenting with connectors on protruding ends
The source of this experiment is "Guide to Molecular Cloning Experiments, Third Edition", translated by Huang Peitang et al.
Targeted cloning experiments in plasmid vectors
Most commonly used plasmids contain multiple cloning sites that can be recognized by different endonucleases. Because of the large number of cloning sites avail
In vitro transcription and translation experiments of cloned genes
DNA cloning refers to the process of molecular manipulation in which a DNA fragment containing a target gene or other meaningful DNA is ligated in vitro with a
Dephosphorylation experiments on enzyme section breaks
In the modification of DNA fragments, the dephosphorylation reaction is an important element, which is catalyzed by alkaline phosphatase, an enzyme that removes
Experiments with the Hanahan method for the preparation and transformation of susceptible Escherichia coli (efficient transformation strategies)
In the late 1970s and early 1980s, when Dong Hanahan was a graduate student at Cold Spring Harbor Laboratory and Harvard University, he made previously unheard
Phage-spreading plate assay for phage spot production
Source of content: compact guide to molecular biology 5th ed.
Multiple sequence alignment and phylogenetic tree construction experiments
Source of content: Guangdong Pharmaceutical University Laboratory Instruction Manual.
Experiments on stable transfection of mammalian cell genes
Analyzing the function of a gene often requires the formation of a mammalian cell line containing the stably integrated gene. In transfection, approximately 1/1