Single-cell mRNA differential display assay
Several methods exist to show changes in the expression levels of unknown genes in tissue samples in response to physiological stimuli. However, the morphologic
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Radiolabeling of minus cDNA probes by random oligonucleotide extension method
The synthesis of cDNA in this protocol was carried out in four saturating concentrations of dNTP and one trace of radiolabeled dNTP. After deduction of hybridiz
Dephosphorylation of DNA fragments by alkaline phosphatase
The source of this experiment is "Guide to Molecular Cloning Experiments, Third Edition", translated by Huang Peitang et al.
Differential display PCR
The source of this experiment is "Guide to Molecular Cloning Experiments, Third Edition", translated by Huang Peitang et al.
Synthesis of non-fixed-length single-stranded DNA probes from M13 phage templates
During synthesis, low concentrations of radiolabeled dNTP limit the length of probes to 200-300 nucleotides. However, newly synthesized DNA can be made 100-1000
In vitro transcriptional synthesis of single-stranded RNA probes
Not only is it easier to prepare specific single-stranded RNA probes than DNA probes, but they also generally produce stronger signals in hybridization reaction
Synthesis of cDNA probes from mRNA using random oligonucleotide primers
In this protocol, poly(A)+ RNA is used as a template to synthesize cDNA probes by random priming reaction, and these probes can be used for differential screeni
Synthesis of fixed-length single-stranded DNA probes from M13 phage templates
The source of this experiment is "Guide to Molecular Cloning Experiments, Third Edition", translated by Huang Peitang et al.
Micronucleus assay for detecting DNA damage at the chromosome level
The study of DNA damage at the chromosomal level is an important part of genotoxicology because chromosomal mutations are an important part of the cancer proces