technology that targets genes
Gene pooping is an experimental tool for targeted alteration of genetic information of a species based on embryonic stem cells (ESCs) and homologous recombinati
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Laboratory Protocols & SOPs Library
3,200+ step-by-step lab protocols and SOPs for cell culture, staining, PCR, protein work and microbiology, with reagents, conditions and evaluation criteria.
3228 articles
Preparation of pseudotyped lentiviral vectors resistant to complement inactivation
This experimental protocol describes several methods for generating complement-stabilized lentiviral vectors either by using fusion packaging proteins containin
Lentiviral transduction of hematopoietic cells
Most hematopoietic cells, including hematopoietic stem cells and terminally differentiated cells such as primary T cells and macrophages, are undifferentiated o
Design method for adeno-associated heterotrimeric viral vectors
A A V coat peptide display library, error-prone P C R and D N A recombination . A A V serotypes with different histophilicity can be generated by natural mutati
Production and use of lentiviral vectors based on feline immunodeficiency virus
Lentiviral vectors based on feline immunodeficiency virus (F IV ) are very useful for mediating integrated transgenesis into non-dividing human cells. This chap
Construction of first-generation adenoviral vectors
The possibility of immune responses against proteins encoded by viral vector backbones limits the application of transgene vectors for in vivo expression. Never
Labeling experiments with fluorescence in situ hybridization probes for genomic target sequences
Fluorescence in situ hybridization (FISH) requires nucleic acid probes, including deoxyribonucleic acid (DNA), ribonucleic acid (RNA), or nucleic acid analogs t
Polylysine copolymers for gene delivery
Polylysine and its copolymers have gained wide application as non-viral gene vectors. Although polylysine itself is toxic to cells, the covalent attachment of p
Cyclodextrin-containing polycations for nucleic acid delivery
A large number of non-viral systems have been used for nucleic acid delivery to cultured cells in vitro or to specific cells in vivo. They vary considerably in