Purification of total RNA using the Marligen Total RNA Rapid Purification System
The Rapid Total RNA System by Marligen Biosciences extracts RNA from a variety of samples with very consistent yield and quality. This experiment was derived fr
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Preparation of RNA from plant tissues using Trizol
The following protocol is a modified version of the Trizol protocol for RNA isolation from plant tissues in which a high-salt isopropyl fermentation precipitati
Magnetic particle-based purification of PCR products
MagneSil is a paramagnetic silica particle that acts as a mobile solid-phase material for capturing double-stranded DNA fragments. The target complexes adhering
Purification of PCR fragments from agarose gels
The Wizard SV Gel and PCR Purification System is a method for the direct purification of PCR fragments from agarose gels or from PCR amplification reaction mixt
RNA purification experiments from plant tissues using ConcertPlant reagents
Outlined below is the protocol that comes with ConcertPlant Reagent (Invitrogen). The protocol is not phenol-based, but requires the addition of chloroform. Thi
RNA purification experiments with Trizol
The production of phenol-based reagents for RNA isolation is based on Chomczynski protocols. These protocols are most useful when obtaining RNA from RNAase-rich
Clean purification experiments for stained ends
Automated fluorescence sequencing using ABIPRISMRigDye end chemistry is one of the methods for efficient DN eight sequence determination. Sequence gradients are
RNA preparation from paraffin-embedded fixed tissues
This protocol was published by Godfrey et al. as an improvement on Fisher's protocol. Previously, protocols for purifying RNA from fixed tissues have been publi
Silica membrane-based purification of PCR products
The Wizard SV96 PCR Purification System provides a membrane-based method for high-yield PCR purification from 96-well plates. This experiment was derived from P