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Laboratory Protocols & SOPs Library

3,200+ step-by-step lab protocols and SOPs for cell culture, staining, PCR, protein work and microbiology, with reagents, conditions and evaluation criteria.

3228 articles
Laboratory Protocols & SOPs Library

Allele-specific PCR

Allele-specific PCR, also known as mismatch PCR, amplification refractory mutation system (ARMS), and mismatch amplification mutation assay (MAMA), is an altern

2025-06-10 03:34:04 Updated
Laboratory Protocols & SOPs Library

In situ PCR

In situ PCR (in situ PCR) is a PCR reaction performed in tissue cells, which combines the advantages of in situ hybridization with cellular localization ability

2025-06-10 03:34:04 Updated
Laboratory Protocols & SOPs Library

PCR-Denaturing Gradient Gel Electrophoresis

PCR-denaturing gradient gel electrophoresis is the process of amplifying the target gene through PCR by adding a nucleotide chain of 30-50 GC bases to the 5' en

2025-06-10 03:34:04 Updated
Laboratory Protocols & SOPs Library

Simple allele discrimination PCR

Simple allel-discriminating PCR, which is based on the principle of amplifi-cation refractory mutation system (ARMS), in which primers are designed to introduce

2025-06-10 03:34:04 Updated
Laboratory Protocols & SOPs Library

Allele-specific PCR with L-DNA labeling

L-DNA-labeled allele-specific PCR combines allele-specific PCR with L-DNA-labeled PCR.L-DNA-labeled PCR amplifies DNA with a sequence-determined L-DNA tag.L-DNA

2025-06-10 03:34:04 Updated
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