3,200+ step-by-step lab protocols and SOPs for cell culture, staining, PCR, protein work and microbiology, with reagents, conditions and evaluation criteria.
Allele-specific PCR, also known as mismatch PCR, amplification refractory mutation system (ARMS), and mismatch amplification mutation assay (MAMA), is an altern
In situ PCR (in situ PCR) is a PCR reaction performed in tissue cells, which combines the advantages of in situ hybridization with cellular localization ability
Reverse transcription-polymerase chain reaction (RT-PCR) has improved the sensitivity of RNA detection by several orders of magnitude, making it possible to ana
PCR-denaturing gradient gel electrophoresis is the process of amplifying the target gene through PCR by adding a nucleotide chain of 30-50 GC bases to the 5' en
Simple allel-discriminating PCR, which is based on the principle of amplifi-cation refractory mutation system (ARMS), in which primers are designed to introduce
L-DNA-labeled allele-specific PCR combines allele-specific PCR with L-DNA-labeled PCR.L-DNA-labeled PCR amplifies DNA with a sequence-determined L-DNA tag.L-DNA
Protein gel electrophoresis is commonly used to (1) analyze molecular biology, genetics, and biochemistry (2) preparative techniques (3) partially purify molecu
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