affinity chromatography
The affinity molecules with special structure are made into solid-phase adsorbent and placed in the chromatography column, when the protein mixture to be separa
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Characterization of decoy proteins
The first step in capturing interacting proteins is to construct a decoy protein, which is a fusion of LexA to the target protein. A series of control experimen
Immunoscreening assay
A technique for selecting a target from a population based on the principle of antigen-antibody interaction. For example, antibodies are used to detect proteins
Metallothionein Measurement Experiment
Metallothionein is a short cysteine-rich peptide produced by microorganisms and plants as a metal-binding protein with a high affinity for a wide range of heavy
Metabolic labeling experiments of recombinant proteins
Since host protein synthesis is essentially terminated when recombinant proteins are expressed, in vivo metabolic labeling is a sensitive method for detecting r
Separation of nucleotides by chromatography on ion exchange columns
In this experiment, yeast RNA was used as the material, and the RNA was hydrolyzed into mononucleotide by alkali, then separated by ion exchange column chromato
Electrotransfer experiments on proteins
Many membranes can be used as solid-phase supports for protein electrotransfer, such as diazotized cellulose membranes (DPT, DBM), DEAE-cellulose membranes, nyl
Molecular weight determination of proteins by gel filtration chromatography experiment
Gel filtration is also known as exclusion chromatography, molecular sieve chromatography and gel chromatography. The purpose of this experiment is to understand
Experiments on the preparation of yeast sucrase
Sucrase (inver tase) (β-D-fructofuranoside fructohydrolase) (EC. 3 . 2 . 1 . 26 ) catalyzes the hydrolysis of α-fructofuranoside bonds in non-reducing sugars