Protein expression experiments in mammalian cells
Content from Compact Molecular Biology Laboratory Guide (5th Edition)
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Experiments on protein purification by lectin affinity chromatography
Lectins are proteins that reversibly bind carbohydrates. Because most lectins have at least two carbohydrate binding sites per molecule, they can precipitate gl
Phosphorylation Site Analysis Experiment - Determination of Phosphorylated Amino Acid Types
Source : Proteomics: From Sequence to Function.
Lysis experiments of cultured animal cells, yeast and bacteria for immunoblotting
Source: Laboratory Guide to Proteins and Proteomics
Far Western analysis of protein interaction experiments
When analyzing protein interactions using Far Western blot, the target protein is immobilized on a solid support membrane and then probed with a non-antibody pr
Experiments on lysis of cultured cells by depressurization of nitrogen compartments
Source: Laboratory Guide to Proteins and Proteomics
Experiments on the preparation of yeast extracts
Source:Laboratory Guide to Proteins and Proteomics
Biopharmaceutical Glycosylation Site Assay
N-glycosylation site studies are one of the most important prerequisites for understanding the function of sugar chains
Affinity purification assay of GST fusion proteins
This experiment describes the application of proteins fused to glutathione-S-transferase (GST fusion proteins) to affinity purify other proteins, also known as