Experiments for the determination of the isoelectric point of proteins
The isoelectric point of a protein is one of the important properties of proteins. Determination of isoelectric point of proteins is important for understanding
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Experiments for precise molecular weight determination of proteins
The exact molecular weight of a protein is the sum of all the atomic weights contained in the molecule. Currently, there is one main method used to determine th
Determination of total protein content based on protein elemental composition
Determination of total protein content based on the elemental composition of proteins is a method of direct analysis based on the characteristics of the element
Pentose phosphate pathway
The pentose phosphate pathway is an oxidative catabolism of glucose that produces NADPH and synthesizes ribose phosphate, which provides the raw material for nu
X-ray crystallography
The ability of protein molecules to crystallize has been reported for more than 170 years, and the discovery of X-rays by Rntgen WC in 1895 and X-ray diffractio
GST pull-down analysis
Protein-protein interactions play an important role in a variety of life processes such as biocatalysis, transporter, signaling, immunity, cellular regulation,
Determination of total protein content based on protein light absorption properties
Determination of total protein content based on protein light absorption properties is based on the light absorption properties of different chemical bonds of p
CUT&RUN-seq protocol
Targets and Release Using Nuclease (CUT&RUN) experimental technology is an emerging chromatin analysis method for studying protein-DNA interactions. It uses antibodies to specifically label target proteins, and then uses nucleases bound to antibodies (such as pAG-MNase) to cut DNA fragments that ...
Acid phosphatase activity assay
Acid phosphatase (ACP) is widely found in various cells, and is often abnormally expressed in some cancer cells. Sodium p-nitrophenol phosphate is used as the substrate of ACP, which can be hydrolyzed under alkaline conditions to produce yellow p-nitrophenol, and ACP can be quantitatively ...