3,200+ step-by-step lab protocols and SOPs for cell culture, staining, PCR, protein work and microbiology, with reagents, conditions and evaluation criteria.
Dextrose Tryptone Broth is a non-selective medium which can support the growth of a variety of mesophilic and thermophilic microorganisms. It contains the pH indicator bromcresol purple. Inoculated filter membranes are incubated on this medium at 55°C to detect and enumerate thermophilic "flat ...
Dextrose Tryptone Agar is recommended for the determination of mesophilic and thermophilic bacteria in sweetening agents used in frozen desserts. Bromcresol purple is used as a pH indicator. When isolating thermophilic bacteria use 55°C as a plating temperature.
Dextrose agar is used for the culture of microorganisms with or without added blood. Supplementation with 5% blood provides additional growth factors for fastidious microorganisms. When pH is lowered to 3.5 medium can be used to obtain total counts of yeasts in food.
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