Laboratory Protocols & SOPs Library
- Experiments on the use of oxygen meters Summary(i) Oxygen electrode: It consists of a 10μ diameter platinum wire as the cathode, enclosed in a glass tube with a cross section exposed. Silver-silver chloride is used as the anode, which is covered with a tetrafluoroplastic casing and a polyethylene film of 15-20μ. A 0.5 NKCl solution ...
- Blood plate preparation experiment SummaryIt is suitable for all kinds of bacterial growth, and should be inoculated with blood plate for the separation of general bacterial test specimens. This experiment is from Mudanjiang Medical College, undergraduate 5-year laboratory guide for testing majors.Operation methodBlood plate ...
- Experiments on the use of autoclaves SummaryAutoclaving is the primary means of sterilizing laboratory materials in the laboratory. The main equipment is a steam autoclave, both horizontal and vertical. There are also portable small autoclaves, which are more convenient to use.The principle of autoclaving is to increase the ...
- Inoculation ring, inoculation needle preparation experiment SummaryThe inoculation ring (needle), also known as the platinum ear (needle), is a common tool used in bacteriologic testing. Its correct use is an important basic technology. This experiment is derived from the laboratory instruction of Mudanjiang Medical College undergraduate 5-year testing ...
- Electrochemical analysis experiment SummaryThe ion-selective electrode method is a quantitative analytical method based on the measurement of the electric potential of a cell. The ion-selective electrode and a reference electrode are connected and placed in the electrolyte solution to be measured, which constitutes a measuring ...
- Experiments on the preparation of iron agar medium with Kirschner's disaccharide SummaryIt is used to observe whether the bacteria can ferment glucose, lactose and decompose protein to produce hydrogen sulfide, which is convenient for the preliminary identification of bacteria. This experiment is from Mudanjiang Medical College, undergraduate 5-year laboratory guide for ...
- Chocolate plate preparation experiment SummaryChocolate plate medium is mainly used for the isolation and culture of aerobic bacteria such as Neisseria spp. and Haemophilus spp. Chocolate plate should be prepared by sheep blood or blood free. Because it contains X and V factors, Haemophilus, Neisseria, etc. grow well, all specimens ...
- Scanning Electron Microscope Sample Preparation Experiment SummaryThe resolution of the microscope depends on the wavelength of the light used, the way the electron beam acts on the sample and the imaging principle is different, the scope of application is also different, at present the most commonly used is the transmission electron microscope and ...
- Glass pipette preparation and sterilization experiments SummaryBoth glass and plastic pipettes can be used for tissue culture. The advantage of plastic pipettes is that they are single-use in a single package and do not need to be cleaned or sterilized. Glass pipettes are very inexpensive, but each time they are used, the cotton stopper must be ...