Laboratory Protocols & SOPs Library
- Balanced salt solution preparation experiment SummarySource of content: Tissue Culture and Molecular Cytology Techniques. (Beijing Publishing House)Operation methodExperiment on the preparation of BSSPrincipleBSS is developed from Ringer physiological saline, mainly composed of inorganic salts and glucose.The inorganic ions in BSS are not ...
- Experiments on the effect of reaction time on product formation SummaryIn this experiment, sucrose was used as the substrate to determine the time course curve of the reaction between sucrase and the substrate, i.e., under the optimum conditions of the enzyme reaction, the amount of product generated was measured at certain intervals, and then the time ...
- Assay for the determination of the activity of the enzyme glutamine-alanine transaminase SummaryThe basic principle of the determination of glutamine-alpha transaminase activity; master the method of the determination of glutamine-alpha transaminase activity. (Source: Biochemistry and Molecular Biology Laboratory Guide, Shao Xueling et al., Wuhan University Press, 2003)Operation ...
- mRNA isolation assay SummaryMost mRNAs from mammalian cells have a poly(A) tail at the 3' end, so mRNA can be isolated from large amounts of cellular RNA by oligo(dT)-cellulose affinity chromatography. mRNA templates have to be prepared by the purification step described above when constructing cDNA libraries. For ...
- Preparation of heparin by ion exchange method SummaryHeparin (Heparin) is a class of acidic mucopolysaccharides that are stable to alkali and heat, and exists in organisms as complexes bound to proteins, which are commonly separated by salting out and enzymolysis, and then removed by heating and filtration. Since heparin molecules are ...
- Experiments on the preparation of casein SummarySource: Biochemistry and Molecular Biology Laboratory Guide, Shao Xueling et al, Wuhan University Press, 2003.Operation methodisoelectric point precipitation methodPrincipleThe main protein in cow's milk is casein, with a content of about 35 g/L. Casein is a mixture of a number of ...
- Analysis of peroxidase isoenzymes in medicinal plants and comparative activity experiments SummaryIn this experiment, polyacrylamide gel electrophoresis was used to analyze and compare the peroxide isoenzymes, and their activities were determined by guaiacol method. (Source: Laboratory instruction of biochemistry and molecular biology, Shao Xueling et al, Wuhan University Press, ...
- Experimental inhibition of succinate dehydrogenase and malonate SummarySuccinate dehydrogenase is an important enzyme in the tricarboxylic acid cycle, and the determination of the presence or absence of succinate dehydrogenase activity in cells can be used to preliminarily characterize the existence of the tricarboxylic acid cycle pathway.(Source: ...
- Residue analysis experiments of carbamate insecticides SummaryUrethane is generally non-corrosive and its storage stability is excellent. Its greatest advantage is that it remains in the plant for only a short time, and most carbamates are degraded into the appropriate metabolites, which usually have the same or greater activity as the parent ...