Recombinant Human C12orf50 Protein: Properties & Research Use
Recombinant Human C12orf50 Protein: Properties & Research Use
Recombinant Human C12orf50 Protein is a recombinantly expressed human protein encoded by the C12orf50 gene (chromosome 12 open reading frame 50), produced to support basic and translational research applications requiring a well-characterized, high-purity protein reagent.
- Product Name
- Recombinant Human C12orf50 Protein
- SKU
- rp376423
- Gene
- C12orf50 (Chromosome 12 Open Reading Frame 50)
- Species Origin
- Human (Homo sapiens)
- Protein Type
- Recombinant protein
- Supplier Reference
- absea#PP-9989
What Is C12orf50?
C12orf50 (Chromosome 12 Open Reading Frame 50) is a human gene located on chromosome 12. Like many open reading frame (ORF) genes, C12orf50 encodes a protein whose precise biological function is still under active investigation. Proteins in the ORF category represent an important frontier in functional genomics research, as they may play roles in cellular signaling, metabolism, or structural organization that have yet to be fully characterized.
Recombinant production of C12orf50 protein enables researchers to investigate its biochemical properties, interaction partners, and potential roles in disease or normal physiology in a controlled, reproducible manner. Access to a purified recombinant form is essential for generating reliable experimental data in studies such as protein–protein interaction assays, antibody validation, and structural analyses.
Production and Quality Specifications
Recombinant Human C12orf50 Protein (SKU: rp376423) is produced using established recombinant expression systems designed to yield a functionally relevant protein suitable for downstream research applications. Recombinant proteins of this class are typically expressed in either prokaryotic (e.g., E. coli) or eukaryotic (e.g., HEK293, Sf9 insect cells) systems, followed by affinity chromatography purification to achieve high purity.
Quality control measures for recombinant proteins typically include SDS-PAGE analysis to verify molecular weight and purity, as well as endotoxin testing to ensure suitability for cell-based assays. Researchers should consult the product datasheet for lot-specific purity, concentration, formulation buffer, and storage recommendations to ensure optimal experimental outcomes.
Note: Specific purity percentages, molecular weight values, and formulation details for this product should be confirmed against the official product datasheet available from Aladdin Scientific (Supplier Reference: absea#PP-9989).
Research Applications
Recombinant Human C12orf50 Protein is suitable for a range of research applications commonly associated with recombinant human proteins. Potential uses include:
- Protein–protein interaction studies: Use as bait or prey in pull-down assays or co-immunoprecipitation experiments to identify binding partners and elucidate functional networks.
- Antibody production and validation: Serve as an immunogen for raising antibodies or as a positive control antigen for validating anti-C12orf50 antibodies in Western blot, ELISA, or immunofluorescence assays.
- Structural biology: Provide purified material for X-ray crystallography, cryo-electron microscopy, or NMR studies aimed at resolving the protein's three-dimensional structure.
- Functional genomics and proteomics: Contribute to large-scale efforts to annotate the function of uncharacterized human proteins, supporting gene ontology and pathway analyses.
- Biochemical assays: Enable enzyme activity assays, binding kinetics measurements, or other in vitro biochemical characterization.
Researchers working on chromosome 12-linked conditions or broad proteome annotation projects may find this protein particularly useful as a research tool.
Handling, Storage, and Reconstitution
Proper handling and storage of recombinant proteins are critical to maintaining their biological activity and structural integrity. General best practices for recombinant human proteins include:
- Storage: Store at –20°C or –80°C as recommended on the product datasheet. Avoid repeated freeze–thaw cycles, which can lead to protein aggregation and loss of activity.
- Reconstitution: If supplied in lyophilized form, reconstitute using the buffer specified in the datasheet (commonly sterile PBS or HEPES-based buffers). Allow the protein to equilibrate to room temperature before opening the vial.
- Aliquoting: Prepare single-use aliquots upon first reconstitution to minimize freeze–thaw damage and contamination risk.
- Working concentrations: Determine optimal working concentrations empirically for each application, as requirements vary between assay formats.
Always refer to the specific product datasheet supplied with your order for authoritative storage conditions, recommended buffers, and stability data for lot rp376423.
Ordering Information
Recombinant Human C12orf50 Protein is available from Aladdin Scientific under the following details:
| Parameter | Detail |
|---|---|
| SKU | rp376423 |
| Product Name | Recombinant Human C12orf50 Protein |
| Supplier Reference | absea#PP-9989 |
For pricing, availability, bulk quotations, or custom formulation inquiries, please contact the Aladdin Scientific sales team or visit the product page on the Aladdin Scientific website. Volume discounts and institutional pricing may be available upon request.
Frequently asked questions
What is C12orf50 and why is it of scientific interest?
C12orf50 (Chromosome 12 Open Reading Frame 50) is a human gene encoding a protein whose biological function is not yet fully characterized. It is of scientific interest because uncharacterized ORF proteins represent key targets in functional genomics and proteomics research, where defining their roles can reveal novel cellular mechanisms or disease associations.
What expression system is used to produce Recombinant Human C12orf50 Protein?
The specific expression system used for rp376423 should be confirmed by consulting the official product datasheet (Supplier Reference: absea#PP-9989) available from Aladdin Scientific. Recombinant human proteins are commonly produced in E. coli, HEK293, or insect cell (Sf9) systems depending on requirements for post-translational modifications and downstream applications.
How should I store Recombinant Human C12orf50 Protein to maintain activity?
As a general best practice, recombinant proteins should be stored at –20°C or –80°C and freeze–thaw cycles should be minimized by preparing single-use aliquots. Specific storage conditions, buffer composition, and shelf-life information for rp376423 are detailed in the product datasheet supplied with your order.
Can Recombinant Human C12orf50 Protein be used for antibody production?
Yes, purified recombinant proteins such as C12orf50 are commonly used as immunogens for raising polyclonal or monoclonal antibodies, and as positive control antigens for validating anti-C12orf50 antibodies in Western blot, ELISA, and other immunoassays. Researchers should verify purity and formulation suitability for immunization from the product datasheet.
Is bulk or custom-formulation ordering available for this product?
Aladdin Scientific may offer bulk quantities and custom formulation options for Recombinant Human C12orf50 Protein (rp376423). Interested parties should contact the Aladdin Scientific sales team directly to discuss volume pricing, custom buffer formulations, and delivery timelines.
References
- UniProt Consortium. UniProt: the universal protein knowledgebase. Nucleic Acids Research. Review entry for C12orf50 (Homo sapiens).
- NCBI Gene. C12orf50 chromosome 12 open reading frame 50 [Homo sapiens]. National Center for Biotechnology Information.
- Aladdin Scientific. Recombinant Human C12orf50 Protein, SKU rp376423. Product Datasheet. Supplier Reference absea#PP-9989.
Products are supplied for research and development use only. Not for use in humans, animals, diagnosis, or therapy.