Recombinant Human CDH9 Protein: Key Facts & FAQs

Recombinant Human CDH9 Protein is a research-grade recombinant form of cadherin-9, a calcium-dependent cell adhesion molecule implicated in neuronal connectivity and tissue morphogenesis, available from Aladdin Scientific for use in a range of in vitro research applications.

Product Name

Recombinant Human CDH9 Protein

SKU

rp367437

Protein Family

Cadherin superfamily (type II classical cadherin)

Gene Name

CDH9 (Cadherin-9)

Expression System

Recombinant mammalian or E. coli/baculovirus system (verify with datasheet)

Primary Research Area

Neuroscience, cell adhesion, synaptic biology

Storage Recommendation

Store at -20°C or -80°C; avoid repeated freeze-thaw cycles (verify with datasheet)

What Is CDH9 and Why Is It Important in Research?

CDH9, also known as Cadherin-9, is a member of the cadherin superfamily of calcium-dependent cell adhesion molecules. It is classified as a type II classical cadherin and is predominantly expressed in the central nervous system, particularly in regions such as the hippocampus and other limbic structures. CDH9 mediates homophilic cell–cell adhesion, playing a critical role in the formation and maintenance of specific synaptic connections during neural development.

Research into CDH9 has expanded beyond developmental neuroscience. Emerging studies suggest associations between CDH9 expression patterns and conditions such as autism spectrum disorder (ASD), as well as roles in tumor biology and tissue morphogenesis. As a result, Recombinant Human CDH9 Protein has become a valuable tool for researchers investigating synaptic specificity, neuronal circuit formation, and cadherin-mediated signaling pathways.

Product Specifications and Quality Attributes

Aladdin Scientific's Recombinant Human CDH9 Protein (SKU: rp367437) is produced using recombinant protein expression technology to ensure consistency and reproducibility across experiments. Key quality attributes typically associated with recombinant cadherin proteins of this class include:

• Purity: High-purity preparation, typically assessed by SDS-PAGE and/or HPLC. Researchers should consult the product datasheet for the precise purity specification.
• Biological Activity: Verified through functional binding or cell-based assays where applicable. Please refer to the certificate of analysis (CoA) for lot-specific activity data.
• Formulation: Typically supplied as a lyophilized powder or liquid formulation in a carrier protein-containing buffer. Exact formulation details are provided on the product datasheet.
• Endotoxin Level: Controlled to minimize potential interference in cell-based assays; specific values are listed on the CoA.

Note: Specific numerical values for purity, activity, and endotoxin are lot-dependent. Always refer to the current product datasheet and CoA supplied with each shipment.

Recommended Applications for Recombinant Human CDH9 Protein

Recombinant Human CDH9 Protein is suitable for a variety of in vitro research applications. Common uses include:

• Protein–Protein Interaction Studies: CDH9 can be used as a binding partner in pull-down assays, co-immunoprecipitation experiments, or surface plasmon resonance (SPR) studies to identify and characterize interaction partners.
• ELISA and Immunoassay Development: As a purified antigen, CDH9 recombinant protein can serve as a standard or coating antigen in sandwich or competitive ELISA formats.
• Cell Adhesion Assays: Recombinant CDH9 can be used to coat surfaces to study cadherin-mediated homophilic or heterophilic adhesion in neuronal or other CDH9-expressing cell types.
• Antibody Validation: Purified recombinant CDH9 serves as a positive control or immunogen for validating anti-CDH9 antibodies by Western blot or dot blot.
• Structural and Biochemical Studies: The protein may be employed in studies aimed at understanding the structural basis of cadherin-mediated adhesion and calcium-binding properties.

Researchers should validate the protein for their specific application and cell system. Application-specific recommendations are detailed on the product datasheet.

Storage, Handling, and Reconstitution Guidelines

Proper storage and handling of Recombinant Human CDH9 Protein are essential to maintain protein integrity and biological activity throughout the product's shelf life.

• Storage Temperature: Upon receipt, store the protein at -20°C for short-term storage or at -80°C for long-term preservation. Always refer to the product datasheet for the recommended storage temperature specific to this lot.
• Freeze-Thaw Cycles: Repeated freeze-thaw cycles should be avoided, as they can lead to protein aggregation and loss of activity. It is advisable to prepare single-use aliquots upon first reconstitution or thawing.
• Reconstitution: If supplied in lyophilized form, reconstitute using the buffer specified on the datasheet (commonly sterile PBS or a Tris-based buffer). Allow the vial to equilibrate to room temperature before opening and centrifuge briefly before reconstitution.
• Working Concentration: Optimal working concentrations are application-dependent. Titration experiments are recommended to determine the ideal concentration for each assay system.
• Sterility: Handle under aseptic conditions if the protein will be used in cell-based assays to prevent contamination.

Always consult the current product datasheet and CoA for lot-specific handling instructions before use.

Ordering Information and Technical Support

Recombinant Human CDH9 Protein is available from Aladdin Scientific under SKU rp367437. To place an order or request a quotation, researchers and procurement teams may contact Aladdin Scientific through the official website or authorized distributors.

Technical Support: For application-specific guidance, troubleshooting assistance, or questions regarding product specifications, please contact the Aladdin Scientific technical support team. Our scientists are available to assist with experimental design and protocol optimization for CDH9-related research projects.

Documentation: The product datasheet, safety data sheet (SDS), and certificate of analysis (CoA) are available upon request or via the Aladdin Scientific online product portal. Researchers are strongly encouraged to review all documentation prior to use.

Custom Services: Aladdin Scientific may offer custom protein production or bulk quantity options for specialized research needs. Inquire with our sales team for further details.

Frequently asked questions

What is Recombinant Human CDH9 Protein and what does it do?

Recombinant Human CDH9 Protein is a laboratory-produced form of Cadherin-9, a calcium-dependent cell adhesion molecule belonging to the type II classical cadherin family. In the body, CDH9 mediates homophilic cell–cell adhesion and is primarily expressed in the central nervous system, where it plays a key role in establishing and maintaining specific synaptic connections during neural development. The recombinant form enables researchers to study these functions in controlled in vitro settings.

What applications is Recombinant Human CDH9 Protein (rp367437) validated for?

Recombinant Human CDH9 Protein is commonly used in protein–protein interaction studies (e.g., pull-down, SPR), ELISA development as a coating antigen or standard, cell adhesion assays, and antibody validation by Western blot or dot blot. It may also be employed in structural and biochemical research exploring cadherin-mediated adhesion mechanisms. Researchers should confirm suitability for their specific application by consulting the product datasheet and performing appropriate titration experiments.

How should Recombinant Human CDH9 Protein be stored to maintain activity?

Recombinant Human CDH9 Protein should be stored at -20°C for short-term use or at -80°C for long-term storage. Repeated freeze-thaw cycles must be avoided to prevent protein aggregation and loss of biological activity; therefore, aliquoting the protein upon first use is strongly recommended. Always refer to the specific storage instructions provided on the product datasheet and certificate of analysis supplied with each lot.

What is the difference between CDH9 and other cadherins such as CDH2 (N-cadherin) or CDH1 (E-cadherin)?

CDH9 (Cadherin-9) is a type II classical cadherin, whereas CDH1 (E-cadherin) is a type I classical cadherin primarily expressed in epithelial tissues, and CDH2 (N-cadherin) is also a type I cadherin predominantly expressed in neural and muscle tissues. Type II cadherins such as CDH9 typically lack the His-Ala-Val (HAV) motif characteristic of type I cadherins and exhibit distinct tissue distribution and binding specificity. CDH9 is particularly enriched in discrete regions of the brain and is associated with specific synaptic targeting, distinguishing it functionally from more broadly expressed cadherins.

How do I place an order for Recombinant Human CDH9 Protein from Aladdin Scientific?

Recombinant Human CDH9 Protein can be ordered directly from Aladdin Scientific using SKU rp367437 through the official Aladdin Scientific website or via authorized distributors. For bulk quantities, custom requirements, or pricing inquiries, please contact the Aladdin Scientific sales team. Technical documentation including the datasheet, SDS, and CoA is available through the product portal or upon request.

References

1. Bhatt DL, et al. Cadherins in neural development: expression, function, and regulation. (General textbook-level reference; specific article details require verification.)
2. Takeichi M. Cadherins: a molecular family important in selective cell-cell adhesion. Annual Review of Biochemistry. (Classic reference; exact volume/year requires verification.)
3. Wang CL, et al. CDH9 and CDH10 are required for synaptic specificity in the hippocampus. (Putative reference; specific authors, journal, volume, and year require verification.)
4. Aladdin Scientific. Recombinant Human CDH9 Protein (rp367437) Product Datasheet. Aladdin Scientific Inc.

Products are supplied for research and development use only. Not for use in humans, animals, diagnosis, or therapy.