Recombinant Human CFAP69 Protein: Research Guide & Usage

Recombinant Human CFAP69 Protein is a research-grade recombinant protein derived from the human CFAP69 gene, associated with cilia and flagella function, and is available from Aladdin Scientific (SKU: rp374837) to support studies in ciliary biology, motility disorders, and related cellular pathways.

Product Name

Recombinant Human CFAP69 Protein

SKU

rp374837

Gene / Protein

CFAP69 (Cilia and Flagella Associated Protein 69)

Species

Human (Homo sapiens)

Product Grade

Research grade recombinant protein

Supplier

Aladdin Scientific

Biological Background of CFAP69

CFAP69 (Cilia and Flagella Associated Protein 69) is a protein encoded by the CFAP69 gene in humans. As its name indicates, CFAP69 is associated with the structure and function of cilia and flagella — highly conserved organelles that play essential roles in cell motility, fluid movement, and signal transduction across a wide range of tissues and cell types.

Cilia and flagella are microtubule-based structures whose proper assembly and function depend on a large number of accessory proteins. CFAP69 is believed to participate in the organization or stabilization of axonemal components, though the precise molecular mechanisms remain an active area of investigation. Disruption of cilia-associated proteins, including CFAP69, has been linked to a class of diseases known as ciliopathies, which can manifest as primary ciliary dyskinesia (PCD), infertility, and other developmental anomalies.

Research into CFAP69 and related proteins contributes to the broader understanding of cilia biology, male fertility (given the role of flagella in sperm motility), and the pathogenesis of rare genetic disorders.

Protein Specifications and Quality

The Recombinant Human CFAP69 Protein supplied by Aladdin Scientific (SKU: rp374837) is produced using recombinant expression technology to ensure batch-to-batch consistency and research-grade quality. Researchers should consult the current product datasheet for confirmed details on the following parameters, as specifications may be updated:

• Expression System: Please refer to the product datasheet (consult Aladdin Scientific technical support for confirmed expression host details).
• Purity: Research-grade purity; specific purity percentage and analytical method (e.g., SDS-PAGE) are detailed on the product datasheet.
• Formulation / Buffer: Please refer to the current product datasheet for confirmed buffer composition and any carrier proteins or additives.
• Endotoxin Level: Please refer to the product datasheet for tested endotoxin specification.
• Molecular Weight: Refer to the product datasheet for confirmed apparent and theoretical molecular weight values.

For the most accurate and up-to-date specifications, always download the current Certificate of Analysis (CoA) and datasheet from Aladdin Scientific or contact technical support at the time of ordering.

Handling, Storage, and Stability Guidelines

Proper handling and storage are critical to maintaining the activity and integrity of Recombinant Human CFAP69 Protein. Researchers should follow these general best-practice guidelines for recombinant proteins, and always cross-reference with the product-specific datasheet:

• Upon Receipt: Inspect the shipment immediately. Verify lot number and confirm the product has been maintained at the correct shipping temperature. Document receipt conditions.
• Short-Term Storage: If the protein will be used within a short period (typically days to weeks), store at 4°C according to the datasheet instructions.
• Long-Term Storage: For long-term storage, aliquot the protein into single-use volumes to avoid repeated freeze-thaw cycles, and store at –20°C or –80°C as specified in the datasheet.
• Freeze-Thaw Cycles: Minimize freeze-thaw cycles, as repeated cycling can cause protein aggregation and loss of activity. Prepare working aliquots upon first thaw.
• Reconstitution: If supplied lyophilized, reconstitute with the recommended volume of sterile water or buffer as specified in the datasheet. Allow to equilibrate gently; avoid vigorous vortexing.
• Protein Concentration: Centrifuge briefly upon thawing if precipitation is suspected. Use appropriate methods (e.g., BCA or Bradford assay) to confirm working concentration.

Always wear appropriate personal protective equipment (PPE) when handling recombinant proteins. Consult the Safety Data Sheet (SDS) for detailed hazard information.

Research Applications

Recombinant Human CFAP69 Protein is intended for research use only and is not approved for diagnostic or therapeutic applications in humans. This protein is suitable for a range of in vitro research applications, including but not limited to:

• Protein–Protein Interaction Studies: Use as bait or prey in pull-down assays or co-immunoprecipitation experiments to identify binding partners of CFAP69 within the ciliary proteome.
• Antibody Development and Validation: Serve as an antigen standard for raising, testing, or validating anti-CFAP69 antibodies in immunoassay development (ELISA, Western blot).
• Biochemical Characterization: Conduct enzymatic or binding assays to characterize the functional properties of CFAP69 under defined in vitro conditions.
• Structural Studies: Use in biophysical assays such as circular dichroism (CD), analytical ultracentrifugation, or as a reference standard in mass spectrometry-based proteomics.
• Ciliopathy Research: Investigate the molecular basis of primary ciliary dyskinesia (PCD), male infertility, or other ciliopathy-related phenotypes where CFAP69 function may be implicated.
• Cell-Free Assay Systems: Incorporate into cell-free reconstitution experiments studying axonemal assembly or ciliary motility mechanisms.

Researchers are encouraged to optimize assay conditions (buffer, pH, temperature, protein concentration) empirically for each specific application.

Ordering, Quality Assurance, and Technical Support

Recombinant Human CFAP69 Protein is available exclusively for research use from Aladdin Scientific under SKU rp374837. The following information is provided to assist procurement and laboratory management teams:

• How to Order: This product can be ordered through the Aladdin Scientific website or by contacting the Aladdin Scientific sales team directly. Reference SKU rp374837 when placing orders to ensure correct product fulfillment.
• Quality Assurance: Each lot of Recombinant Human CFAP69 Protein is tested according to Aladdin Scientific's quality control procedures. A Certificate of Analysis (CoA) specific to each lot is available upon request or via the product page.
• Returns and Replacements: For issues relating to product quality or shipment damage, contact Aladdin Scientific customer service promptly upon receipt. Retain all original packaging for return claims.
• Technical Support: Aladdin Scientific technical support staff are available to advise on storage conditions, reconstitution protocols, and suitable assay conditions. Contact technical support via the Aladdin Scientific website for application-specific guidance.
• Regulatory Statement: This product is for Research Use Only (RUO). Not for use in diagnostic procedures or clinical applications.

Frequently asked questions

What is CFAP69 and why is it relevant to cilia research?

CFAP69 (Cilia and Flagella Associated Protein 69) is a protein implicated in the structure and function of cilia and flagella. Research on CFAP69 is relevant to understanding ciliary motility, ciliopathies such as primary ciliary dyskinesia (PCD), and conditions like male infertility where sperm flagella function is critical.

How should Recombinant Human CFAP69 Protein be stored to maintain its activity?

For short-term use, store at 4°C. For long-term storage, aliquot into single-use volumes and store at –20°C or –80°C to minimize freeze-thaw cycles. Always consult the product-specific datasheet and Certificate of Analysis (CoA) for confirmed storage recommendations, as conditions may vary by lot and formulation.

What are the recommended research applications for Recombinant Human CFAP69 Protein?

This protein is suitable for in vitro research applications including protein–protein interaction studies (pull-down, co-IP), antibody development and validation (ELISA, Western blot), biochemical characterization, structural studies, and ciliopathy research. It is for Research Use Only and is not intended for diagnostic or therapeutic use.

Can Recombinant Human CFAP69 Protein be used in cell-based or in vivo assays?

This product is validated for in vitro research use. For any cell-based applications, researchers should empirically optimize conditions such as protein concentration, buffer, and delivery method. The product is not approved for in vivo or clinical use. Please contact Aladdin Scientific technical support for guidance on specific application suitability.

How do I request a Certificate of Analysis (CoA) for my lot of Recombinant Human CFAP69 Protein?

A lot-specific Certificate of Analysis (CoA) for Recombinant Human CFAP69 Protein (SKU: rp374837) can be obtained through the Aladdin Scientific product page or by contacting Aladdin Scientific customer service with your lot number. The CoA provides confirmed specifications including purity, formulation, and quality control test results.

References

1. Reiter JF, Leroux MR. Genes and molecular pathways underpinning ciliopathies. Nature Reviews Molecular Cell Biology. 2017;18(9):533-547. doi:10.1038/nrm.2017.60
2. Pazour GJ, Agrin N, Leszyk J, Witman GB. Proteomic analysis of a eukaryotic cilium. Journal of Cell Biology. 2005;170(1):103-113. doi:10.1083/jcb.200504008
3. Wallmeier J, et al. Motile ciliopathies and their associated genes. Cold Spring Harbor Perspectives in Biology. 2020;12(12):a036681. doi:10.1101/cshperspect.a036681

Products are supplied for research and development use only. Not for use in humans, animals, diagnosis, or therapy.