Recombinant Human FBLN1 Protein: Properties & Applications

Recombinant Human FBLN1 Protein is a research-grade extracellular matrix glycoprotein that enables scientists to investigate fibulin-1's roles in cell adhesion, matrix assembly, and tumor suppression with high reproducibility and lot-to-lot consistency.

Protein Name

Fibulin-1 (FBLN1)

SKU

rp367866

Species

Human (Homo sapiens)

Protein Family

Fibulin family of extracellular matrix glycoproteins

Key Biological Functions

Cell adhesion, extracellular matrix assembly, tumor suppression, fibronectin and laminin binding

Form

Recombinant protein (research use only)

Biological Background of FBLN1

Fibulin-1 (FBLN1) is a secreted extracellular matrix (ECM) glycoprotein and a founding member of the fibulin protein family. It is widely expressed in basement membranes and connective tissues throughout the body, where it plays critical structural and regulatory roles. FBLN1 interacts with a broad range of ECM components, including fibronectin, laminin, nidogen, and fibrinogen, contributing to the organization and stability of the extracellular matrix.

At the molecular level, FBLN1 contains multiple epidermal growth factor (EGF)-like repeat domains and a C-terminal fibulin-type module, which mediate its protein–protein interactions. These structural features allow FBLN1 to function as an adaptor molecule bridging different ECM constituents. Beyond structural roles, FBLN1 modulates cell adhesion, migration, and proliferation, making it a subject of extensive research in developmental biology and disease.

FBLN1 has attracted considerable attention as a putative tumor suppressor. Reduced expression of FBLN1 has been reported in several human cancers, and experimental restoration of FBLN1 expression has been shown to inhibit tumor cell growth and invasion in vitro. These findings position FBLN1 as both a potential biomarker and a functional target in oncology research.

Protein Structure and Isoforms

The FBLN1 gene encodes multiple splice variants, with four major isoforms described in the literature: FBLN1A, FBLN1B, FBLN1C, and FBLN1D. These isoforms differ primarily in their C-terminal regions, which affects their binding partners and functional properties. FBLN1C and FBLN1D are the most abundantly expressed isoforms in most adult human tissues.

All FBLN1 isoforms share a common domain architecture that includes an N-terminal anaphylatoxin-like module, nine tandem EGF-like domains (with the majority being calcium-binding EGF-like repeats), and a C-terminal fibulin-type module. The EGF-like repeats are central to FBLN1's calcium-dependent interactions with ECM ligands and cell-surface receptors. Post-translational modifications, including N-linked glycosylation, are important for the proper folding, secretion, and function of the native protein.

When working with recombinant FBLN1, researchers should note that the isoform expressed, host expression system, and glycosylation status can influence experimental outcomes. Aladdin Scientific's Recombinant Human FBLN1 Protein (rp367866) is produced under controlled conditions designed to deliver a biologically relevant and functionally active protein for in vitro research applications.

Research Applications

Recombinant Human FBLN1 Protein is a versatile reagent used across multiple research disciplines. Key application areas include:

• Cell Adhesion and Migration Assays: FBLN1 can be used as a substrate or soluble factor to study its effects on cell attachment, spreading, and directional migration in vitro.
• Extracellular Matrix Reconstitution: Researchers incorporate recombinant FBLN1 into cell culture systems or 3D ECM models to recapitulate aspects of the native tissue microenvironment.
• Protein–Protein Interaction Studies: Recombinant FBLN1 is used in pull-down assays, co-immunoprecipitation, and surface plasmon resonance (SPR) experiments to map its binding partners and determine interaction kinetics.
• Cancer Biology: FBLN1 is employed in studies examining its tumor-suppressive activity, including proliferation, invasion, and colony formation assays in cancer cell lines.
• ELISA and Immunoassay Development: As a purified antigen standard, recombinant FBLN1 supports the development and validation of FBLN1-specific immunoassays and antibody characterization.
• Western Blot and Functional Controls: Recombinant FBLN1 serves as a positive control for Western blot experiments and functional assays requiring a defined protein standard.

Quality, Storage, and Handling

For reliable experimental outcomes, researchers should adhere to recommended storage and handling conditions for Recombinant Human FBLN1 Protein (rp367866). As with most recombinant proteins, repeated freeze-thaw cycles should be avoided to preserve activity and integrity. Upon receipt, it is advisable to aliquot the protein into single-use volumes and store at the temperature recommended on the product datasheet.

Purity and activity are critical quality attributes for any research-grade recombinant protein. Aladdin Scientific's FBLN1 protein is subject to quality control testing to confirm purity and biological activity prior to release. Researchers are encouraged to consult the Certificate of Analysis (CoA) provided with each lot for specific purity, concentration, and activity data.

When reconstituting lyophilized protein, use the buffer specified in the product datasheet, allow the protein to dissolve gently, and avoid vigorous vortexing to prevent aggregation. Carrier proteins such as BSA may be added to improve stability during long-term storage in dilute solutions. Always handle the protein under sterile conditions if it will be used in cell-based assays.

Ordering Information and Product Specifications

Recombinant Human FBLN1 Protein is available from Aladdin Scientific under SKU rp367866. This product is intended for research use only and is not suitable for diagnostic or therapeutic applications in humans or animals.

For detailed specifications including molecular weight, expression host, tag information, formulation, concentration, and validated applications, please refer to the official product datasheet available on the Aladdin Scientific website or contact our technical support team. Custom formulations or bulk quantities may be available upon request.

Researchers requiring additional technical guidance on experimental design, optimal concentration ranges, or troubleshooting are encouraged to contact Aladdin Scientific's scientific support team. Complementary products, including anti-FBLN1 antibodies and related ECM proteins, are also available in the Aladdin Scientific catalog to support comprehensive research programs.

Frequently asked questions

What is FBLN1 and why is it important in research?

FBLN1 (Fibulin-1) is a secreted extracellular matrix glycoprotein involved in cell adhesion, ECM assembly, and tumor suppression. Its reduced expression in multiple cancers and its interactions with key ECM proteins such as fibronectin and laminin make it an important target in cancer biology, developmental biology, and matrix biology research.

What applications is Recombinant Human FBLN1 Protein (rp367866) validated for?

Recombinant Human FBLN1 Protein (rp367866) is suitable for applications including cell adhesion and migration assays, protein–protein interaction studies, ELISA antigen standards, Western blot positive controls, and extracellular matrix reconstitution experiments. Please consult the product datasheet for the full list of validated applications and recommended working concentrations.

How should Recombinant Human FBLN1 Protein be stored to maintain its activity?

To maintain protein activity and integrity, avoid repeated freeze-thaw cycles by aliquoting the protein into single-use volumes upon receipt. Store at the temperature specified on the product datasheet. For dilute solutions intended for long-term storage, adding a carrier protein such as BSA is recommended. Always refer to the specific storage instructions on the CoA provided with your lot.

Is Recombinant Human FBLN1 Protein suitable for in vivo or clinical use?

No. Recombinant Human FBLN1 Protein (rp367866) from Aladdin Scientific is strictly for research use only. It is not approved, tested, or validated for diagnostic, therapeutic, or any other clinical applications in humans or animals.

Are there different isoforms of FBLN1 and does this affect my experiment?

Yes, FBLN1 has four main splice isoforms (FBLN1A, B, C, D) that differ at their C-termini and exhibit different binding properties and tissue expression patterns. The isoform present in a recombinant preparation can influence experimental outcomes, particularly in binding studies. Researchers should confirm which isoform is represented in the product and consider this when designing and interpreting experiments.

References

1. Timpl R, Sasaki T, Kostka G, Chu ML. Fibulins: a versatile family of extracellular matrix proteins. Nature Reviews Molecular Cell Biology. 2003;4(6):479-489.
2. Argraves WS, Tran H, Burgess WH, Bhatt K. Fibulin is an extracellular matrix and plasma glycoprotein with repeated domain structure. Journal of Cell Biology. 1990;111(6):3155-3164.
3. Twal WO, Czirok A, Hegedus B, et al. Fibulin-1 suppression of fibronectin-regulated cell adhesion and motility. Journal of Cell Science. 2001;114(Pt 24):4587-4598.
4. Qing J, et al. Fibulin-1 is a potential tumor suppressor gene in various cancers: a review of the literature. Oncology Letters. (Representative review article — specific volume/year requires verification.)

Products are supplied for research and development use only. Not for use in humans, animals, diagnosis, or therapy.