Recombinant Human FBLN2 Protein: Research Guide & Protocol

Recombinant Human FBLN2 Protein is a high-quality extracellular matrix research reagent produced for use in a broad range of biochemical and cell-biological studies exploring fibulin-2 function, basement membrane assembly, and matrix-associated signaling pathways.

Product Name

Recombinant Human FBLN2 Protein

SKU

rp365517

Protein Family

Fibulin family (extracellular matrix glycoproteins)

Primary Function

Basement membrane assembly, ECM organization, and modulation of cell adhesion and migration

Species Origin

Human (Homo sapiens)

Recommended Storage

Avoid repeated freeze-thaw cycles; store at -80°C for long-term use (verify exact conditions on product datasheet)

Key Applications

ELISA, Western blot, cell-based assays, binding studies, and functional ECM research

Background: FBLN2 Biology and Significance

Fibulin-2 (FBLN2) is a secreted extracellular matrix (ECM) glycoprotein belonging to the fibulin family, which is characterized by tandem arrays of epidermal growth factor (EGF)-like domains and a C-terminal fibulin-type module. FBLN2 is encoded by the FBLN2 gene and is expressed in a variety of tissues, including the heart, blood vessels, and connective tissues, where it plays an integral role in ECM remodeling and basement membrane assembly.

Fibulin-2 interacts with multiple ECM components such as fibronectin, nidogen, versican, and aggrecan, as well as with structural proteins of the basement membrane including laminin and type IV collagen. These interactions facilitate the physical scaffolding of tissues and modulate key cellular processes such as adhesion, migration, and differentiation. FBLN2 has been implicated in cardiovascular development and disease, tumor suppression, and tissue fibrosis, making it a target of significant research interest across oncology, cardiology, and developmental biology.

Recombinant Human FBLN2 Protein provides a standardized, reproducible source of this ECM component, enabling investigators to dissect its molecular interactions and functional roles in controlled experimental systems.

Recommended Handling and Storage Protocol

Proper handling of Recombinant Human FBLN2 Protein is essential to maintain activity and integrity throughout your experiments. Follow the guidelines below:

• Upon Receipt: Inspect the vial immediately. If the product is shipped on dry ice, allow it to equilibrate briefly before opening. Record the lot number and expiration date.
• Reconstitution: If supplied in lyophilized form, reconstitute using the volume and buffer specified in the product datasheet. Gently mix by pipetting; avoid vigorous vortexing to prevent denaturation. Allow the protein to dissolve at room temperature for the time indicated.
• Short-Term Storage: For experiments within 1–2 weeks, store at 4°C in an appropriate carrier protein buffer (e.g., 0.1% BSA in PBS) to minimize adsorption losses.
• Long-Term Storage: Aliquot into single-use volumes and store at -80°C. Avoid repeated freeze-thaw cycles, as these degrade protein activity and structural integrity.
• Working Concentration: Titrate the optimal working concentration empirically for each assay. Refer to the product datasheet for suggested starting ranges.
• Sterility: For cell-based assays, use aseptic technique throughout; filter-sterilize reconstituted protein if required.

Note: Always consult the official Aladdin Scientific product datasheet (SKU: rp365517) for lot-specific conditions, as formulation details may vary between batches.

Experimental Applications

Recombinant Human FBLN2 Protein is suitable for a range of research applications. Common uses include:

• ELISA and Immunoassays: Use as a standard or capture/detection antigen to develop or validate sandwich ELISA systems measuring FBLN2 in biological samples.
• Western Blot (WB): Serve as a positive control to confirm antibody specificity and sensitivity in immunoblotting workflows.
• Protein–Protein Interaction Studies: Employ in pull-down, co-immunoprecipitation, or surface plasmon resonance (SPR) assays to characterize FBLN2 binding partners such as nidogen, fibronectin, and versican.
• Cell-Based Functional Assays: Coat culture surfaces or add exogenously to media to study the effects of FBLN2 on cell adhesion, migration, proliferation, and differentiation in relevant cell types (e.g., vascular smooth muscle cells, fibroblasts, tumor cells).
• ECM Reconstitution Experiments: Incorporate into in vitro basement membrane models to interrogate matrix assembly and remodeling dynamics.
• Antibody Development and Validation: Use as an immunogen or validation antigen for anti-FBLN2 antibody production and characterization.

Optimization of assay conditions, including protein concentration, buffer composition, and incubation parameters, is recommended for each application.

Quality, Purity, and Regulatory Considerations

When selecting recombinant proteins for critical research applications, quality attributes are paramount. For Recombinant Human FBLN2 Protein (rp365517) from Aladdin Scientific, researchers should verify the following on the product datasheet:

• Purity: Confirm purity grade (typically assessed by SDS-PAGE and/or HPLC). Research-grade proteins are generally ≥90% pure; confirm the lot-specific value on the certificate of analysis (CoA).
• Biological Activity: Review any reported functional activity data (e.g., binding EC50 values from ELISA or SPR) provided in the datasheet or CoA.
• Endotoxin Level: For cell-based assays, endotoxin content is critical. Confirm LAL-tested endotoxin values on the CoA to ensure suitability for your application.
• Host Expression System: The expression system (e.g., HEK293 cells, CHO cells, E. coli, or baculovirus) affects glycosylation and folding. Verify the expression host on the datasheet to ensure relevance to your experimental model.
• Formulation: Note buffer composition, carrier proteins, and any stabilizers present, as these may affect downstream assay compatibility.

Aladdin Scientific recommends always requesting the lot-specific CoA to confirm all quality parameters before initiating critical experiments. For regulatory or GMP-grade requirements, contact Aladdin Scientific customer support directly.

Ordering and Support Information

Recombinant Human FBLN2 Protein is available from Aladdin Scientific under SKU rp365517. To place an order or obtain additional technical support:

• Online Ordering: Visit the Aladdin Scientific website and search by SKU rp365517 or product name to add to your cart and proceed through standard procurement workflows.
• Technical Support: For application-specific guidance, custom formulation inquiries, or large-volume pricing, contact the Aladdin Scientific technical support team via the website contact form or direct email.
• Documentation: The full product datasheet, safety data sheet (SDS), and certificate of analysis (CoA) are available for download on the product page. Always use the lot-specific CoA for quality verification.
• Custom Requirements: Aladdin Scientific can accommodate requests for custom concentrations, buffer exchange, or bulk quantities. Inquire with the sales team for lead times and pricing.

For procurement teams: standard terms for payment, shipping, and returns are outlined on the Aladdin Scientific website. Volume discounts and institutional pricing may be available — contact your regional Aladdin Scientific representative for details.

Frequently asked questions

What is FBLN2 and why is it important in research?

FBLN2 (fibulin-2) is a secreted extracellular matrix glycoprotein involved in basement membrane assembly, ECM organization, and regulation of cell adhesion and migration. It has been implicated in cardiovascular development, tumor suppression, and fibrosis, making it relevant across oncology, cardiology, and developmental biology research fields.

How should Recombinant Human FBLN2 Protein be stored to maintain its activity?

For long-term storage, aliquot the protein and store at -80°C to avoid repeated freeze-thaw cycles, which degrade activity. For short-term use within 1–2 weeks, store at 4°C in a buffer containing a carrier protein such as 0.1% BSA. Always refer to the lot-specific product datasheet for exact storage and formulation conditions.

What applications is Recombinant Human FBLN2 Protein validated for?

Recombinant Human FBLN2 Protein (rp365517) is suitable for applications including ELISA, Western blot as a positive control, protein–protein interaction studies (pull-down, co-IP, SPR), cell-based adhesion and migration assays, ECM reconstitution models, and antibody development and validation. Optimal conditions should be determined empirically for each specific assay.

How do I know the purity and quality of this recombinant protein?

Each lot of Recombinant Human FBLN2 Protein is accompanied by a Certificate of Analysis (CoA) that provides lot-specific data on purity (typically assessed by SDS-PAGE and/or HPLC), endotoxin levels (LAL test), and biological activity where applicable. Download the CoA from the Aladdin Scientific product page for SKU rp365517 or request it from customer support.

Can Recombinant Human FBLN2 Protein be used in cell culture experiments?

Yes, Recombinant Human FBLN2 Protein can be used in cell-based assays to study effects on adhesion, migration, proliferation, and differentiation. For cell culture use, verify endotoxin levels on the CoA are within acceptable limits for your cell type, and employ aseptic technique throughout handling and reconstitution.

References

1. Kobayashi N, et al. Fibulin-2 and fibulin-5 define the epithelial mesenchymal boundary in mouse embryos. Developmental Biology. (General reference for FBLN2 biology — specific details require verification.)
2. Pan TC, et al. Structure and expression of fibulin-2, a novel extracellular matrix protein with multiple EGF-like repeats and consensus motifs for calcium binding. Journal of Cell Biology. (General fibulin-2 structural reference — specific volume/page details require verification.)
3. Aladdin Scientific. Product Datasheet: Recombinant Human FBLN2 Protein, SKU rp365517. Aladdin Scientific (absea#PP-1095). Retrieved from Aladdin Scientific website.

Products are supplied for research and development use only. Not for use in humans, animals, diagnosis, or therapy.