Recombinant Human GEMIN5 Protein: FAQs & Key Facts

Recombinant Human GEMIN5 Protein is a key component of the SMN (Survival of Motor Neuron) complex involved in snRNP biogenesis and RNA metabolism, now available from Aladdin Scientific (SKU: rp375077) for use in biochemical and cell biology research applications.

Product Name

Recombinant Human GEMIN5 Protein

SKU

rp375077

Protein Function

Component of the SMN complex; involved in snRNP assembly and RNA binding

Species Origin

Human (Homo sapiens)

Application

Biochemical assays, protein interaction studies, RNA biology research

Supplier Reference

absea#PP-8810

What Is GEMIN5 and Why Is It Important?

GEMIN5 (Gem-associated protein 5) is a large WD-repeat domain-containing protein that functions as a subunit of the SMN (Survival of Motor Neuron) complex. The SMN complex is essential for the biogenesis of small nuclear ribonucleoproteins (snRNPs), which are core components of the spliceosome responsible for pre-mRNA splicing. GEMIN5 directly recognizes and binds the Sm-site sequence on snRNAs, playing a critical role in guiding Sm protein ring assembly onto snRNA. Disruption of SMN complex function is associated with Spinal Muscular Atrophy (SMA), a severe neuromuscular disease. Recombinant Human GEMIN5 Protein enables researchers to study these processes in vitro without the complexity of endogenous cell extracts.

Product Specifications and Quality Attributes

The Recombinant Human GEMIN5 Protein available from Aladdin Scientific (SKU: rp375077, supplier reference absea#PP-8810) is produced recombinantly to support consistent, reproducible experimental results. Researchers should consult the product datasheet for confirmed details on the following attributes, as specific lot-level data may vary:

• Expression System: Please refer to the current datasheet for the confirmed host expression system (e.g., mammalian, baculovirus, or E. coli).
• Purity: Purity grade and analytical method (e.g., SDS-PAGE, HPLC) are specified on the product datasheet.
• Formulation: Buffer composition and any carrier proteins or stabilizers are detailed in the Certificate of Analysis (CoA).
• Molecular Weight: GEMIN5 is a high-molecular-weight protein; exact recombinant construct size should be verified in the datasheet.

For the most accurate and up-to-date specifications, always reference the lot-specific CoA available upon request from Aladdin Scientific.

Storage, Handling, and Stability

Proper storage and handling of recombinant proteins are critical for maintaining biological activity and experimental reproducibility. General best-practice guidelines for Recombinant Human GEMIN5 Protein include:

• Short-term storage: Store at 4°C if the protein will be used within 1–2 weeks of receipt.
• Long-term storage: Aliquot and store at −20°C or −80°C to avoid repeated freeze-thaw cycles, which can degrade protein integrity and activity.
• Freeze-thaw cycles: Minimize freeze-thaw cycles; prepare single-use aliquots at the time of first use.
• Carrier protein: If the formulation does not contain a stabilizer, consider adding BSA (0.1% final concentration) to working aliquots to prevent adsorption to tube surfaces.

Specific storage recommendations are provided on the product datasheet and CoA. Always consult these documents upon receipt of the product.

Recommended Research Applications

Recombinant Human GEMIN5 Protein is suitable for a range of in vitro biochemical and molecular biology applications, including but not limited to:

• snRNA binding assays: Characterizing the RNA-binding specificity of GEMIN5 toward Sm-site-containing snRNAs.
• Protein–protein interaction studies: Investigating GEMIN5 interactions with other SMN complex subunits (e.g., SMN, GEMIN2–8, UNRIP) using pull-down, co-immunoprecipitation, or SPR assays.
• In vitro snRNP assembly: Reconstituting steps of the snRNP assembly pathway using defined recombinant components.
• SMA disease modeling: Studying mechanisms underlying Spinal Muscular Atrophy at the molecular level.
• Structural biology: Providing purified protein for crystallography or cryo-EM studies of SMN complex architecture.

Researchers should validate the suitability of this protein for their specific experimental conditions using appropriate positive and negative controls.

Ordering, Shipping, and Support

Recombinant Human GEMIN5 Protein (SKU: rp375077) is available through Aladdin Scientific. The following information applies to ordering and logistics:

• Ordering: Place orders via the Aladdin Scientific website, by email, or through your institutional procurement system. Reference SKU rp375077 to ensure correct product selection.
• Shipping: Recombinant proteins are typically shipped on dry ice to maintain cold-chain integrity. Confirm shipping conditions with Aladdin Scientific at the time of order.
• Returns and refunds: For quality concerns or damaged shipments, contact Aladdin Scientific customer service promptly upon receipt. Refer to the standard returns and refunds policy.
• Technical support: For application-specific guidance, protocol assistance, or datasheet requests, contact the Aladdin Scientific technical support team.

Frequently asked questions

What is the biological function of GEMIN5?

GEMIN5 is a subunit of the SMN (Survival of Motor Neuron) complex and functions as an snRNA-binding protein that recognizes the Sm-site on small nuclear RNAs. It plays an essential role in the assembly of snRNPs, which are core components of the spliceosome. GEMIN5 dysfunction has been linked to Spinal Muscular Atrophy (SMA).

What applications is Recombinant Human GEMIN5 Protein validated for?

Recombinant Human GEMIN5 Protein is suitable for in vitro biochemical applications including snRNA binding assays, protein–protein interaction studies (pull-down, co-IP, SPR), in vitro snRNP reconstitution, and structural biology studies. Researchers are advised to consult the product datasheet and validate the protein for their specific assay conditions.

How should Recombinant Human GEMIN5 Protein be stored to maintain activity?

For short-term use (within 1–2 weeks), store at 4°C. For long-term storage, aliquot the protein and store at −20°C or −80°C to avoid repeated freeze-thaw cycles. Always refer to the lot-specific datasheet and Certificate of Analysis for confirmed storage recommendations.

What is the catalog number (SKU) for Recombinant Human GEMIN5 Protein at Aladdin Scientific?

The catalog number (SKU) for Recombinant Human GEMIN5 Protein at Aladdin Scientific is rp375077. The supplier reference number is absea#PP-8810. Please use SKU rp375077 when placing your order to ensure correct product selection.

Is Recombinant Human GEMIN5 Protein suitable for use in SMA research?

Yes, GEMIN5 is a component of the SMN complex whose dysfunction is directly associated with Spinal Muscular Atrophy (SMA). Recombinant Human GEMIN5 Protein can be used to study SMN complex assembly, snRNP biogenesis, and molecular mechanisms relevant to SMA in vitro. Researchers should validate the protein in their specific SMA model system.

References

1. Gubitz AK, Feng W, Dreyfuss G. The SMN complex. Experimental Cell Research. 2004;296(1):51-56. doi:10.1016/j.yexcr.2004.03.022
2. Battle DJ, Lau CK, Wan L, Deng H, Lotti F, Dreyfuss G. The Gemin5 protein of the SMN complex identifies snRNAs. Molecular Cell. 2006;23(2):273-279. doi:10.1016/j.molcel.2006.05.048
3. Lau CK, Bachorik JL, Dreyfuss G. Gemin5-snRNA interaction reveals an RNA binding function for WD repeat domains. Nature Structural and Molecular Biology. 2009;16(5):486-491. doi:10.1038/nsmb.1584

Products are supplied for research and development use only. Not for use in humans, animals, diagnosis, or therapy.